The pluripotency state of human embryonic stem cells derived from single blastomeres of eight-cell embryos

Human embryonic stem cells (hESCs) derived from blastocyst stage embryos present a primed state of pluripotency, whereas mouse ESCs (mESCs) display naïve pluripotency. Their unique characteristics make naïve hESCs more suitable for particular applications in biomedical research. This work aimed to d...

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Detalles Bibliográficos
Autores: Massafret Surinyach, Ot|||0000-0002-7511-0568, Barragán, Montserrat, Álvarez-González, Lucía|||0000-0001-8154-8614, Aran, Begoña|||0000-0002-5011-7755, Martín-Mur, Beatriz|||0000-0002-6006-3295, Esteve-Codina, Anna|||0000-0003-0361-2873, Ruiz Herrera Moreno, Aurora|||0000-0003-3868-6151, Ibáñez, Elena|||0000-0002-0275-6958, Santaló, Josep|||0000-0002-1698-9699
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:300327
Acceso en línea:https://ddd.uab.cat/record/300327
https://dx.doi.org/urn:doi:10.1016/j.cdev.2024.203935
Access Level:acceso abierto
Palabra clave:Hesc
Derivation
Blastomeres
Naïve
Primed
Pluripotency
Descripción
Sumario:Human embryonic stem cells (hESCs) derived from blastocyst stage embryos present a primed state of pluripotency, whereas mouse ESCs (mESCs) display naïve pluripotency. Their unique characteristics make naïve hESCs more suitable for particular applications in biomedical research. This work aimed to derive hESCs from single blastomeres and determine their pluripotency state, which is currently unclear. We derived hESC lines from single blastomeres of 8-cell embryos and from whole blastocysts, and analysed several naïve pluripotency indicators, their transcriptomic profile and their trilineage differentiation potential. No significant differences were observed between blastomere-derived hESCs (bm-hESCs) and blastocyst-derived hESCs (bc-hESCs) for most naïve pluripotency indicators, including TFE3 localization, mitochondrial activity, and global DNA methylation and hydroxymethylation, nor for their trilineage differentiation potential. Nevertheless, bm-hESCs showed an increased single-cell clonogenicity and a higher expression of naïve pluripotency markers at early passages than bc-hESCs. Furthermore, RNA-seq revealed that bc-hESCs overexpressed a set of genes related to the post-implantational epiblast. Altogether, these results suggest that bm-hESCs, although displaying primed pluripotency, would be slightly closer to the naïve end of the pluripotency continuum than bc-hESCs.

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