Catalase, superoxide dismutase and butylated hydroxytoluene benefit mid-term storage of red-legged partridge sperm (Alectoris rufa)

9 Pág.

Detalles Bibliográficos
Autores: Bernal Juárez, Berenice, Gallarosa-García, Isabel, Toledano-Díaz, Adolfo, Castaño, Cristina, Velázquez, Rosario, Torres, Olga, García-Gil, María, Santiago-Moreno, Julián
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/369526
Acceso en línea:http://hdl.handle.net/10261/369526
Access Level:acceso abierto
Palabra clave:Cryopreservation
Catalase
Chilling
SOD
BHT
ROS
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spelling Catalase, superoxide dismutase and butylated hydroxytoluene benefit mid-term storage of red-legged partridge sperm (Alectoris rufa)Bernal Juárez, BereniceGallarosa-García, IsabelToledano-Díaz, AdolfoCastaño, CristinaVelázquez, RosarioTorres, OlgaGarcía-Gil, MaríaSantiago-Moreno, JuliánCryopreservationCatalaseChillingSODBHTROS9 Pág.The present study assessed the effect of different antioxidants on the quality of chilled/frozen-thawed sperm of red-legged partridge. Sperm samples from 40 red-legged partridges were collected and extended 1:1 (v:v) with Lake and Ravie 84, supplemented with ascorbic acid or butylated hydroxytoluene (BHT) at 0, 0.2, 0.4, 0.8 mM and catalase (CAT) or superoxide dismutase (SOD) at 0, 100, 200 and 300 IU/ml. Ten sperm samples were used per concentration. Motility and viability were evaluated in fresh and after 6 h of chilling at 5°C or after freezing-thawing. For chilled sperm, the presence of ascorbic acid decreased viability and several motility variables; BHT 0.8 mM increased non-progressive motility (NPM, 26.7 ± 1.99 vs. 20.7 ± 2.12); CAT 200 IU/ml improved the rectilinear velocity (40.4 ± 4.63 μ/s vs. 29.9 ± 4.62 μ/s) and linear progression ratio (52.8 ± 3.11% vs. 45.4 ± 2.98%); SOD 100 IU/ml increased NPM (24.5 ± 1.21% vs. 19.3 ± 1.75%) and tended to improve total progressive motility (42.7 ± 3.33% vs. 33.2 ± 3.26%, p = 0.07). Using an extender supplemented with CAT 200 or SOD 100 did not improve the post-thawed sperm quality. The present work provides an advance in the optimisation of chilling and freezing protocols for red-legged partridge sperm.This research was funded by the project RTA2017-00034-00-00. INIA [RTA2017-00034-00-00]Peer reviewedTaylor & FrancisCSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Ministerio de Ciencia e Innovación (España)Bernal Juárez, Berenice [0000-0001-9144-1554]Toledano-Díaz, A. [0000-0001-6679-485X]Castaño, Cristina [0000-0003-1134-1436]Velázquez, Rosario [0009-0009-2115-4715]Torres, Olga [0000-0002-4867-6443]Santiago Moreno, Julián [0000-0001-5551-8120]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/369526reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MICINN/RTA2017-00034-00-00/Departamento de Reproducción Animal​ Departamento de ​Mejora Genética Animalhttps://doi.org/10.1080/00071668.2024.2394185Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3695262026-05-22T06:33:51Z
dc.title.none.fl_str_mv Catalase, superoxide dismutase and butylated hydroxytoluene benefit mid-term storage of red-legged partridge sperm (Alectoris rufa)
title Catalase, superoxide dismutase and butylated hydroxytoluene benefit mid-term storage of red-legged partridge sperm (Alectoris rufa)
spellingShingle Catalase, superoxide dismutase and butylated hydroxytoluene benefit mid-term storage of red-legged partridge sperm (Alectoris rufa)
Bernal Juárez, Berenice
Cryopreservation
Catalase
Chilling
SOD
BHT
ROS
title_short Catalase, superoxide dismutase and butylated hydroxytoluene benefit mid-term storage of red-legged partridge sperm (Alectoris rufa)
title_full Catalase, superoxide dismutase and butylated hydroxytoluene benefit mid-term storage of red-legged partridge sperm (Alectoris rufa)
title_fullStr Catalase, superoxide dismutase and butylated hydroxytoluene benefit mid-term storage of red-legged partridge sperm (Alectoris rufa)
title_full_unstemmed Catalase, superoxide dismutase and butylated hydroxytoluene benefit mid-term storage of red-legged partridge sperm (Alectoris rufa)
title_sort Catalase, superoxide dismutase and butylated hydroxytoluene benefit mid-term storage of red-legged partridge sperm (Alectoris rufa)
dc.creator.none.fl_str_mv Bernal Juárez, Berenice
Gallarosa-García, Isabel
Toledano-Díaz, Adolfo
Castaño, Cristina
Velázquez, Rosario
Torres, Olga
García-Gil, María
Santiago-Moreno, Julián
author Bernal Juárez, Berenice
author_facet Bernal Juárez, Berenice
Gallarosa-García, Isabel
Toledano-Díaz, Adolfo
Castaño, Cristina
Velázquez, Rosario
Torres, Olga
García-Gil, María
Santiago-Moreno, Julián
author_role author
author2 Gallarosa-García, Isabel
Toledano-Díaz, Adolfo
Castaño, Cristina
Velázquez, Rosario
Torres, Olga
García-Gil, María
Santiago-Moreno, Julián
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Ministerio de Ciencia e Innovación (España)
Bernal Juárez, Berenice [0000-0001-9144-1554]
Toledano-Díaz, A. [0000-0001-6679-485X]
Castaño, Cristina [0000-0003-1134-1436]
Velázquez, Rosario [0009-0009-2115-4715]
Torres, Olga [0000-0002-4867-6443]
Santiago Moreno, Julián [0000-0001-5551-8120]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cryopreservation
Catalase
Chilling
SOD
BHT
ROS
topic Cryopreservation
Catalase
Chilling
SOD
BHT
ROS
description 9 Pág.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/369526
url http://hdl.handle.net/10261/369526
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MICINN/RTA2017-00034-00-00/
Departamento de Reproducción Animal​ 
Departamento de ​Mejora Genética Animal
https://doi.org/10.1080/00071668.2024.2394185
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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