Regulation of yeast fatty acid desaturase in response to iron deficiency

Unsaturated fatty acids (UFA) are essential components of phospholipids that greatly contribute to the biophysical properties of cellular membranes. Biosynthesis of UFAs relies on a conserved family of iron-dependent fatty acid desaturases, whose representative in the model yeast Saccharomyces cerev...

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Autores: Romero, Antonia M., Jordá, Tania, Rozès, Nicolas, Martínez-Pastor, María Teresa, Puig, Sergi
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/164001
Acesso em linha:http://hdl.handle.net/10261/164001
Access Level:acceso abierto
Palavra-chave:Yeast
Saccharomyces cerevisiae
Iron deficiency
Fatty acids
Ole1
Mga2
Hypoxia
Cold
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spelling Regulation of yeast fatty acid desaturase in response to iron deficiencyRomero, Antonia M.Jordá, TaniaRozès, NicolasMartínez-Pastor, María TeresaPuig, SergiYeastSaccharomyces cerevisiaeIron deficiencyFatty acidsOle1Mga2HypoxiaColdUnsaturated fatty acids (UFA) are essential components of phospholipids that greatly contribute to the biophysical properties of cellular membranes. Biosynthesis of UFAs relies on a conserved family of iron-dependent fatty acid desaturases, whose representative in the model yeast Saccharomyces cerevisiae is Ole1. OLE1 expression is tightly regulated to adapt UFA biosynthesis and lipid bilayer properties to changes in temperature, and in UFA or oxygen availability. Despite iron deficiency being the most extended nutritional disorder worldwide, very little is known about the mechanisms and the biological relevance of fatty acid desaturases regulation in response to iron starvation. In this report, we show that endoplasmic reticulum-anchored transcription factor Mga2 activates OLE1 transcription in response to nutritional and genetic iron deficiencies. Cells lacking MGA2 display low UFA levels and do not grow under iron-limited conditions, unless UFAs are supplemented or OLE1 is overexpressed. The proteasome, E3 ubiquitin ligase Rsp5 and the Cdc48Npl4/Ufd1 complex are required for OLE1 activation during iron depletion. Interestingly, Mga2 also activates the transcription of its own mRNA in response to iron deficiency, hypoxia, low temperature and low UFAs. MGA2 up-regulation contributes to increase OLE1 expression in these situations. These results reveal the mechanism of OLE1 regulation when iron is scarce and identify the MGA2 auto-regulation as a potential activation strategy in multiple stresses.This work was supported by the predoctoral contract BES-2012-055637 from the Spanish Ministry of Economy, Industry and Competitiveness to Antonia M. Romero; and the Spanish Ministry of Economy, Industry and Competitiveness grants BIO2014-56298-P and BIO2017-87828-C2-1-P, and FEDER (Fondo Europeo de Desarrollo Regional) funds to Sergi Puig.Peer reviewedElsevierMinisterio de Economía y Competitividad (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/164001reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2014-56298-Pinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-87828-C2-1-Phttps://doi.org/10.1016/j.bbalip.2018.03.008Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1640012026-05-22T06:33:51Z
dc.title.none.fl_str_mv Regulation of yeast fatty acid desaturase in response to iron deficiency
title Regulation of yeast fatty acid desaturase in response to iron deficiency
spellingShingle Regulation of yeast fatty acid desaturase in response to iron deficiency
Romero, Antonia M.
Yeast
Saccharomyces cerevisiae
Iron deficiency
Fatty acids
Ole1
Mga2
Hypoxia
Cold
title_short Regulation of yeast fatty acid desaturase in response to iron deficiency
title_full Regulation of yeast fatty acid desaturase in response to iron deficiency
title_fullStr Regulation of yeast fatty acid desaturase in response to iron deficiency
title_full_unstemmed Regulation of yeast fatty acid desaturase in response to iron deficiency
title_sort Regulation of yeast fatty acid desaturase in response to iron deficiency
dc.creator.none.fl_str_mv Romero, Antonia M.
Jordá, Tania
Rozès, Nicolas
Martínez-Pastor, María Teresa
Puig, Sergi
author Romero, Antonia M.
author_facet Romero, Antonia M.
Jordá, Tania
Rozès, Nicolas
Martínez-Pastor, María Teresa
Puig, Sergi
author_role author
author2 Jordá, Tania
Rozès, Nicolas
Martínez-Pastor, María Teresa
Puig, Sergi
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Yeast
Saccharomyces cerevisiae
Iron deficiency
Fatty acids
Ole1
Mga2
Hypoxia
Cold
topic Yeast
Saccharomyces cerevisiae
Iron deficiency
Fatty acids
Ole1
Mga2
Hypoxia
Cold
description Unsaturated fatty acids (UFA) are essential components of phospholipids that greatly contribute to the biophysical properties of cellular membranes. Biosynthesis of UFAs relies on a conserved family of iron-dependent fatty acid desaturases, whose representative in the model yeast Saccharomyces cerevisiae is Ole1. OLE1 expression is tightly regulated to adapt UFA biosynthesis and lipid bilayer properties to changes in temperature, and in UFA or oxygen availability. Despite iron deficiency being the most extended nutritional disorder worldwide, very little is known about the mechanisms and the biological relevance of fatty acid desaturases regulation in response to iron starvation. In this report, we show that endoplasmic reticulum-anchored transcription factor Mga2 activates OLE1 transcription in response to nutritional and genetic iron deficiencies. Cells lacking MGA2 display low UFA levels and do not grow under iron-limited conditions, unless UFAs are supplemented or OLE1 is overexpressed. The proteasome, E3 ubiquitin ligase Rsp5 and the Cdc48Npl4/Ufd1 complex are required for OLE1 activation during iron depletion. Interestingly, Mga2 also activates the transcription of its own mRNA in response to iron deficiency, hypoxia, low temperature and low UFAs. MGA2 up-regulation contributes to increase OLE1 expression in these situations. These results reveal the mechanism of OLE1 regulation when iron is scarce and identify the MGA2 auto-regulation as a potential activation strategy in multiple stresses.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/164001
url http://hdl.handle.net/10261/164001
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2014-56298-P
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-87828-C2-1-P
https://doi.org/10.1016/j.bbalip.2018.03.008
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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