The making of versatile peroxidase by directed evolution

Versatile peroxidase (VP) secreted by white-rot fungi is involved in the degradation of lignin within land ecosystems. With a broad substrate scope and minor requirements, VP is an extremely attractive blueprint to be designed by the directed evolution tool-box. In recent years, improved VP variants...

Descripción completa

Detalles Bibliográficos
Autores: González-Pérez, David, Alcalde Galeote, Miguel
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/169221
Acceso en línea:http://hdl.handle.net/10261/169221
Access Level:acceso abierto
Palabra clave:Versatile peroxidase
Directed evolution
Saccharomyces cerevisiae
Functional expression
Stability
Activity
id ES_bc06a031cca5eb2b98b4f83b2bc19244
oai_identifier_str oai:digital.csic.es:10261/169221
network_acronym_str ES
network_name_str España
repository_id_str
spelling The making of versatile peroxidase by directed evolutionGonzález-Pérez, DavidAlcalde Galeote, MiguelVersatile peroxidaseDirected evolutionSaccharomyces cerevisiaeFunctional expressionStabilityActivityVersatile peroxidase (VP) secreted by white-rot fungi is involved in the degradation of lignin within land ecosystems. With a broad substrate scope and minor requirements, VP is an extremely attractive blueprint to be designed by the directed evolution tool-box. In recent years, improved VP variants have been generated that meet industrial requirements in terms of improved heterologous functional expression and activity, as well as stability at high temperatures or in the presence of strong inhibitors. This review describes the making of VP by directed evolution, addressing the most important findings and challenges that were faced, along with the future prospects for this research field.European UnionFP7-KBBE-2013-7-613549-INDOXCOST Action CM1303 Spanish Government BIO2016-79106-R- Lignolution Seventh Framework Programme10.13039/100011102FP7-KBBE-2013-7-613549-INDOXEuropean Cooperation in Science and Technology10.13039/501100000921CM1303 Systems BiocatalysisThis work was supported by grants from the European Union [FP7-KBBE-2013-7-613549-INDOX], the COST Action [CM1303 Systems Biocatalysis] and the Spanish Government [BIO2016-79106-R-Lignolution].Peer reviewedTaylor & FrancisEuropean CommissionMinisterio de Ciencia e Innovación (España)European Cooperation in Science and TechnologyConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/169221reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/FP7/613549info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-79106-Rhttps://doi.org/10.1080/10242422.2017.1363190Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1692212026-05-22T06:33:51Z
dc.title.none.fl_str_mv The making of versatile peroxidase by directed evolution
title The making of versatile peroxidase by directed evolution
spellingShingle The making of versatile peroxidase by directed evolution
González-Pérez, David
Versatile peroxidase
Directed evolution
Saccharomyces cerevisiae
Functional expression
Stability
Activity
title_short The making of versatile peroxidase by directed evolution
title_full The making of versatile peroxidase by directed evolution
title_fullStr The making of versatile peroxidase by directed evolution
title_full_unstemmed The making of versatile peroxidase by directed evolution
title_sort The making of versatile peroxidase by directed evolution
dc.creator.none.fl_str_mv González-Pérez, David
Alcalde Galeote, Miguel
author González-Pérez, David
author_facet González-Pérez, David
Alcalde Galeote, Miguel
author_role author
author2 Alcalde Galeote, Miguel
author2_role author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Ciencia e Innovación (España)
European Cooperation in Science and Technology
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Versatile peroxidase
Directed evolution
Saccharomyces cerevisiae
Functional expression
Stability
Activity
topic Versatile peroxidase
Directed evolution
Saccharomyces cerevisiae
Functional expression
Stability
Activity
description Versatile peroxidase (VP) secreted by white-rot fungi is involved in the degradation of lignin within land ecosystems. With a broad substrate scope and minor requirements, VP is an extremely attractive blueprint to be designed by the directed evolution tool-box. In recent years, improved VP variants have been generated that meet industrial requirements in terms of improved heterologous functional expression and activity, as well as stability at high temperatures or in the presence of strong inhibitors. This review describes the making of VP by directed evolution, addressing the most important findings and challenges that were faced, along with the future prospects for this research field.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/169221
url http://hdl.handle.net/10261/169221
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/FP7/613549
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-79106-R
https://doi.org/10.1080/10242422.2017.1363190
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418077686333440
score 15.811543

Similares en LA Referencia