Regional difference in inflammatory response to LPS-injection in the brain: Role of microglia cell density

To elucidate whether density of cells could contribute to the extent of microglial activation, we performed in vitro assays using three different densities of N13 microglia stimulated with LPS. Our results showed that induction of pro-inflammatory factors as TNF-α and iNOS was directly related to ce...

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Detalles Bibliográficos
Autores: Pintado, Cristina, Revilla, Elisa, Vizuete, Marisa, Jiménez-Reyes, Sebastián, García Cuervo, Luisa, Vitorica, Javier, Ruano, Diego, Castaño, Angélica
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/72811
Acceso en línea:http://hdl.handle.net/10261/72811
Access Level:acceso abierto
Palabra clave:Neurodegeneration
Striatum
Substantia nigra
Microglia density
Inflammation
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spelling Regional difference in inflammatory response to LPS-injection in the brain: Role of microglia cell densityPintado, CristinaRevilla, ElisaVizuete, MarisaJiménez-Reyes, SebastiánGarcía Cuervo, LuisaVitorica, JavierRuano, DiegoCastaño, AngélicaNeurodegenerationStriatumSubstantia nigraMicroglia densityInflammationTo elucidate whether density of cells could contribute to the extent of microglial activation, we performed in vitro assays using three different densities of N13 microglia stimulated with LPS. Our results showed that induction of pro-inflammatory factors as TNF-α and iNOS was directly related to cell density, meanwhile the induction of the anti-inflammatory IL-10 was inversely related to cell density. Accordingly, in vivo assays showed that after LPS-injection, iNOS expression was more intense in substantia nigra, a brain area showing specific susceptibility to neurodegeneration after microglia activation, whereas IL-10 expression was more sustained in striatum, an area resistant to damage. These results support that microglia density is pivotal to control the balance between pro- and anti-inflammatory factors release. © 2011 Elsevier B.V.This study was supported by (1) the Fondo de Investigación Sanitaria (FIS), from Instituto de Salud Carlos III of Spain, through grants PI060781 (to D.R.) and PI060567 (to J.V.), and by the (2) Junta de Andalucía, Proyecto de Excelencia CVI-902 (to J.V.). C.P. was recipient of a fellowship from Ministerio de Educación y Ciencia (MEC) of Spain.Peer ReviewedElsevier2013201320112013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/72811reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.jneuroim.2011.06.017info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/728112026-05-22T06:33:51Z
dc.title.none.fl_str_mv Regional difference in inflammatory response to LPS-injection in the brain: Role of microglia cell density
title Regional difference in inflammatory response to LPS-injection in the brain: Role of microglia cell density
spellingShingle Regional difference in inflammatory response to LPS-injection in the brain: Role of microglia cell density
Pintado, Cristina
Neurodegeneration
Striatum
Substantia nigra
Microglia density
Inflammation
title_short Regional difference in inflammatory response to LPS-injection in the brain: Role of microglia cell density
title_full Regional difference in inflammatory response to LPS-injection in the brain: Role of microglia cell density
title_fullStr Regional difference in inflammatory response to LPS-injection in the brain: Role of microglia cell density
title_full_unstemmed Regional difference in inflammatory response to LPS-injection in the brain: Role of microglia cell density
title_sort Regional difference in inflammatory response to LPS-injection in the brain: Role of microglia cell density
dc.creator.none.fl_str_mv Pintado, Cristina
Revilla, Elisa
Vizuete, Marisa
Jiménez-Reyes, Sebastián
García Cuervo, Luisa
Vitorica, Javier
Ruano, Diego
Castaño, Angélica
author Pintado, Cristina
author_facet Pintado, Cristina
Revilla, Elisa
Vizuete, Marisa
Jiménez-Reyes, Sebastián
García Cuervo, Luisa
Vitorica, Javier
Ruano, Diego
Castaño, Angélica
author_role author
author2 Revilla, Elisa
Vizuete, Marisa
Jiménez-Reyes, Sebastián
García Cuervo, Luisa
Vitorica, Javier
Ruano, Diego
Castaño, Angélica
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Neurodegeneration
Striatum
Substantia nigra
Microglia density
Inflammation
topic Neurodegeneration
Striatum
Substantia nigra
Microglia density
Inflammation
description To elucidate whether density of cells could contribute to the extent of microglial activation, we performed in vitro assays using three different densities of N13 microglia stimulated with LPS. Our results showed that induction of pro-inflammatory factors as TNF-α and iNOS was directly related to cell density, meanwhile the induction of the anti-inflammatory IL-10 was inversely related to cell density. Accordingly, in vivo assays showed that after LPS-injection, iNOS expression was more intense in substantia nigra, a brain area showing specific susceptibility to neurodegeneration after microglia activation, whereas IL-10 expression was more sustained in striatum, an area resistant to damage. These results support that microglia density is pivotal to control the balance between pro- and anti-inflammatory factors release. © 2011 Elsevier B.V.
publishDate 2011
dc.date.none.fl_str_mv 2011
2013
2013
2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/72811
url http://hdl.handle.net/10261/72811
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.jneuroim.2011.06.017
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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