DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis

Bipolar spindle organization is essential for the faithful segregation of chromosomes during cell division. This organization relies on the collective activities of motor proteins. The minus-end-directed dynein motor complex generates spindle inward forces and plays a major role in spindle pole focu...

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Authors: Rosas Salvans, Miquel, 1987-, Scrofani, Jacopo, 1984-, Modol, Aitor, Vernos, Isabelle, 1959-
Format: article
Status:Published version
Publication Date:2019
Country:España
Institution:Universitat Pompeu Fabra
Repository:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/42307
Online Access:http://hdl.handle.net/10230/42307
http://dx.doi.org/10.1242/jcs.227033
Access Level:Open access
Keyword:DnaJB6
Dynein
Microtubules
RanGTP
Spindle organization
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spelling DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosisRosas Salvans, Miquel, 1987-Scrofani, Jacopo, 1984-Modol, AitorVernos, Isabelle, 1959-DnaJB6DyneinMicrotubulesRanGTPSpindle organizationBipolar spindle organization is essential for the faithful segregation of chromosomes during cell division. This organization relies on the collective activities of motor proteins. The minus-end-directed dynein motor complex generates spindle inward forces and plays a major role in spindle pole focusing. The dynactin complex regulates many dynein functions, increasing its processivity and force production. Here, we show that DnaJB6 is a novel RanGTP-regulated protein. It interacts with the dynactin subunit p150Glued (also known as DCTN1) in a RanGTP-dependent manner specifically in M-phase, and promotes spindle pole focusing and dynein force generation. Our data suggest a novel mechanism by which RanGTP regulates dynein activity during M-phase.We acknowledge the Spanish Ministry of Economy, Industry and Competitiveness (Ministerio de Economía y Competitividad) to the EMBL partnership and support of the Spanish Ministry of Economy and Competitiveness, ‘Centro de Excelencia Severo Ochoa’ as well as support of the CERCA Programme/Generalitat de Catalunya. M.R. was supported by a FPI fellowship from the Spanish Ministry of Economy BES-2013-064601. Work in the Vernos laboratory was supported by grants from the Spanish Ministry of Economy and Competitiveness I+D (grants BFU2012-37163 and BFU2015-68726-P). Deposited in PMC for immediate release.Company of Biologists201920192019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/42307http://dx.doi.org/10.1242/jcs.227033reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésJ Cell Sci. 2019 Jun 3; 132(11)info:eu-repo/grantAgreement/ES/1PE/BES-2013-064601info:eu-repo/grantAgreement/ES/3PN/BFU2012-37163info:eu-repo/grantAgreement/ES/1PE/BFU2015-68726-P© Company of Biologists. http://dx.doi.org/10.1242/jcs.227033. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributedhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/423072026-06-12T07:21:37Z
dc.title.none.fl_str_mv DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis
title DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis
spellingShingle DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis
Rosas Salvans, Miquel, 1987-
DnaJB6
Dynein
Microtubules
RanGTP
Spindle organization
title_short DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis
title_full DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis
title_fullStr DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis
title_full_unstemmed DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis
title_sort DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis
dc.creator.none.fl_str_mv Rosas Salvans, Miquel, 1987-
Scrofani, Jacopo, 1984-
Modol, Aitor
Vernos, Isabelle, 1959-
author Rosas Salvans, Miquel, 1987-
author_facet Rosas Salvans, Miquel, 1987-
Scrofani, Jacopo, 1984-
Modol, Aitor
Vernos, Isabelle, 1959-
author_role author
author2 Scrofani, Jacopo, 1984-
Modol, Aitor
Vernos, Isabelle, 1959-
author2_role author
author
author
dc.subject.none.fl_str_mv DnaJB6
Dynein
Microtubules
RanGTP
Spindle organization
topic DnaJB6
Dynein
Microtubules
RanGTP
Spindle organization
description Bipolar spindle organization is essential for the faithful segregation of chromosomes during cell division. This organization relies on the collective activities of motor proteins. The minus-end-directed dynein motor complex generates spindle inward forces and plays a major role in spindle pole focusing. The dynactin complex regulates many dynein functions, increasing its processivity and force production. Here, we show that DnaJB6 is a novel RanGTP-regulated protein. It interacts with the dynactin subunit p150Glued (also known as DCTN1) in a RanGTP-dependent manner specifically in M-phase, and promotes spindle pole focusing and dynein force generation. Our data suggest a novel mechanism by which RanGTP regulates dynein activity during M-phase.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/42307
http://dx.doi.org/10.1242/jcs.227033
url http://hdl.handle.net/10230/42307
http://dx.doi.org/10.1242/jcs.227033
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv J Cell Sci. 2019 Jun 3; 132(11)
info:eu-repo/grantAgreement/ES/1PE/BES-2013-064601
info:eu-repo/grantAgreement/ES/3PN/BFU2012-37163
info:eu-repo/grantAgreement/ES/1PE/BFU2015-68726-P
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
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