Enzymatic Starch Quantification in Developing Flower Primordia of Sweet Cherry

14 pags.- 5 Figs.- 2 Tabls. © 2025 The Authors; exclusive licensee Bio-protocol LLC. This is an open access article under the CC BY-NC license.

Detalhes bibliográficos
Autores: Santolaria, Nestor, Fadón Adrián, Erica, Rodrigo García, Javier, Hedhly, Afif
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2025
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::cda1601b2d38603e74a3902353d2bd2a
Acesso em linha:http://hdl.handle.net/10261/428578
Access Level:Acceso aberto
Palavra-chave:Starch enzymatic assay
Flower primordia
HK/G6PD assay
NADH spectrophotometric assay
Sweet cherry
Temperate fruit trees
sweet cherries
flowering
bio-assays
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spelling Enzymatic Starch Quantification in Developing Flower Primordia of Sweet CherrySantolaria, NestorFadón Adrián, EricaRodrigo García, JavierHedhly, AfifStarch enzymatic assayFlower primordiaHK/G6PD assayNADH spectrophotometric assaySweet cherryTemperate fruit treessweet cherriesfloweringbio-assays14 pags.- 5 Figs.- 2 Tabls. © 2025 The Authors; exclusive licensee Bio-protocol LLC. This is an open access article under the CC BY-NC license.Starch is a carbohydrate widely used in the plant kingdom as a fuel for different physiological processes. While different techniques are available for the quantification of starch stored in seeds and bark tissues, they have hardly been used to quantify starch content in developing flower buds, where starch has been reported to accumulate in different reproductive organs. Here, we detail a quantitative enzymatic method to measure starch concentration in developing flower primordia in sweet cherry (Prunus avium L.). First, starch is enzymatically hydrolyzed to D-glucose, which was then quantified by an enzyme-coupled assay involving hexokinase (HK) and glucose-6-phosphate dehydrogenase (G6PD) and spectrophotometric quantification of NADH absorbance at 340 nm. This method is a sensitive, rapid, and affordable protocol specifically optimized for tiny flower buds with low starch content. The technique is revealed to successfully determine starch content in non-freshly harvested samples—frozen and stored at -20 °C or stored in fixatives—allowing a temporal separation of sampling and quantification and making the protocol suitable for high-throughput experimental designs in different fields of plant research.This work was funded by the R&D&I project PID2020-115473RR-I00 financed by MICIU/AEI/10.13039/501100011033 and the Government of Aragón – European Social Fund, ‘The ESF invests in your future’ [Consolidated Group A12–17R].Peer reviewedBio-protocolMinisterio de Ciencia, Innovación y Universidades (España)Gobierno de AragónEuropean CommissionHedhly, Afif [0000-0002-4761-3731]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/428578reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115473RR-I00https://doi.org/10.21769/BioProtoc.5256Síinfo:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::cda1601b2d38603e74a3902353d2bd2a2026-05-22T06:33:51Z
dc.title.none.fl_str_mv Enzymatic Starch Quantification in Developing Flower Primordia of Sweet Cherry
title Enzymatic Starch Quantification in Developing Flower Primordia of Sweet Cherry
spellingShingle Enzymatic Starch Quantification in Developing Flower Primordia of Sweet Cherry
Santolaria, Nestor
Starch enzymatic assay
Flower primordia
HK/G6PD assay
NADH spectrophotometric assay
Sweet cherry
Temperate fruit trees
sweet cherries
flowering
bio-assays
title_short Enzymatic Starch Quantification in Developing Flower Primordia of Sweet Cherry
title_full Enzymatic Starch Quantification in Developing Flower Primordia of Sweet Cherry
title_fullStr Enzymatic Starch Quantification in Developing Flower Primordia of Sweet Cherry
title_full_unstemmed Enzymatic Starch Quantification in Developing Flower Primordia of Sweet Cherry
title_sort Enzymatic Starch Quantification in Developing Flower Primordia of Sweet Cherry
dc.creator.none.fl_str_mv Santolaria, Nestor
Fadón Adrián, Erica
Rodrigo García, Javier
Hedhly, Afif
author Santolaria, Nestor
author_facet Santolaria, Nestor
Fadón Adrián, Erica
Rodrigo García, Javier
Hedhly, Afif
author_role author
author2 Fadón Adrián, Erica
Rodrigo García, Javier
Hedhly, Afif
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Gobierno de Aragón
European Commission
Hedhly, Afif [0000-0002-4761-3731]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Starch enzymatic assay
Flower primordia
HK/G6PD assay
NADH spectrophotometric assay
Sweet cherry
Temperate fruit trees
sweet cherries
flowering
bio-assays
topic Starch enzymatic assay
Flower primordia
HK/G6PD assay
NADH spectrophotometric assay
Sweet cherry
Temperate fruit trees
sweet cherries
flowering
bio-assays
description 14 pags.- 5 Figs.- 2 Tabls. © 2025 The Authors; exclusive licensee Bio-protocol LLC. This is an open access article under the CC BY-NC license.
publishDate 2025
dc.date.none.fl_str_mv 2025
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/428578
url http://hdl.handle.net/10261/428578
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115473RR-I00
https://doi.org/10.21769/BioProtoc.5256
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Bio-protocol
publisher.none.fl_str_mv Bio-protocol
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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