Implication of miR-155-5p and miR-143-3p in the Vascular Insulin Resistance and Instability of Human and Experimental Atherosclerotic Plaque
(1) Background: Cardiovascular diseases (CVDs) are the main cause of death in developed countries, being atherosclerosis, a recurring process underlying their apparition. MicroRNAs (miRNAs) modulate the expression of their targets and have emerged as key players in CVDs; (2) Methods: 18 miRNAs were...
| Autores: | , , , , , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de publicación: | 2022 |
| País: | España |
| Recursos: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/93718 |
| Acesso em linha: | https://hdl.handle.net/20.500.14352/93718 |
| Access Level: | acceso abierto |
| Palavra-chave: | 577.2 atherosclerosis miR-155-5p miR-143-3p unstable plaque apoptosis AKT IGF-IIR Biología molecular (Farmacia) Bioquímica (Farmacia) 2302 Bioquímica 2415 Biología Molecular |
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Implication of miR-155-5p and miR-143-3p in the Vascular Insulin Resistance and Instability of Human and Experimental Atherosclerotic PlaqueGonzález-López, PaulaAres-Carral, CarlaLópez-Pastor, Andrea R.Infante-Menéndez, JorgeGonzález Illanes, TamaraVega de Ceniga, MelinaEsparza, LeticiaBeneit, NuriaMartín-Ventura, José LuisEscribano Illanes, ÓscarGómez Hernández, María De La Almudena577.2atherosclerosismiR-155-5pmiR-143-3punstable plaqueapoptosisAKTIGF-IIRBiología molecular (Farmacia)Bioquímica (Farmacia)2302 Bioquímica2415 Biología Molecular(1) Background: Cardiovascular diseases (CVDs) are the main cause of death in developed countries, being atherosclerosis, a recurring process underlying their apparition. MicroRNAs (miRNAs) modulate the expression of their targets and have emerged as key players in CVDs; (2) Methods: 18 miRNAs were selected (Pubmed and GEO database) for their possible role in promoting atherosclerosis and were analysed by RT-qPCR in the aorta from apolipoprotein E-deficient (ApoE−/−) mice. Afterwards, the altered miRNAs in the aorta from 18 weeks-ApoE−/− mice were studied in human aortic and carotid samples; (3) Results: miR-155-5p was overexpressed and miR-143-3p was downregulated in mouse and human atherosclerotic lesions. In addition, a significant decrease in protein kinase B (AKT), target of miR-155-5p, and an increase in insulin-like growth factor type II receptor (IGF-IIR), target of miR-143-3p, were noted in aortic roots from ApoE−/− mice and in carotid plaques from patients with advanced carotid atherosclerosis (ACA). Finally, the overexpression of miR-155-5p reduced AKT levels and its phosphorylation in vascular smooth muscle cells, while miR-143-3p overexpression decreased IGF-IIR reducing apoptosis in vascular cells; (4) Conclusions: Our results suggest that miR-155-5p and miR-143-3p may be implicated in insulin resistance and plaque instability by the modulation of their targets AKT and IGF-IIR, contributing to the progression of atherosclerosis.MDPIUniversidad Complutense de Madrid20222022-09-0620222022-09-06journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/93718reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengRTI-2018-095098-B100 Not available Not availablePID2021-123076OB-I00 Not available Not availablePR75 18-21572 Not availableAENC1 22-29754 Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/937182026-06-02T12:44:21Z |
| dc.title.none.fl_str_mv |
Implication of miR-155-5p and miR-143-3p in the Vascular Insulin Resistance and Instability of Human and Experimental Atherosclerotic Plaque |
| title |
Implication of miR-155-5p and miR-143-3p in the Vascular Insulin Resistance and Instability of Human and Experimental Atherosclerotic Plaque |
| spellingShingle |
Implication of miR-155-5p and miR-143-3p in the Vascular Insulin Resistance and Instability of Human and Experimental Atherosclerotic Plaque González-López, Paula 577.2 atherosclerosis miR-155-5p miR-143-3p unstable plaque apoptosis AKT IGF-IIR Biología molecular (Farmacia) Bioquímica (Farmacia) 2302 Bioquímica 2415 Biología Molecular |
| title_short |
Implication of miR-155-5p and miR-143-3p in the Vascular Insulin Resistance and Instability of Human and Experimental Atherosclerotic Plaque |
| title_full |
Implication of miR-155-5p and miR-143-3p in the Vascular Insulin Resistance and Instability of Human and Experimental Atherosclerotic Plaque |
| title_fullStr |
Implication of miR-155-5p and miR-143-3p in the Vascular Insulin Resistance and Instability of Human and Experimental Atherosclerotic Plaque |
| title_full_unstemmed |
Implication of miR-155-5p and miR-143-3p in the Vascular Insulin Resistance and Instability of Human and Experimental Atherosclerotic Plaque |
| title_sort |
Implication of miR-155-5p and miR-143-3p in the Vascular Insulin Resistance and Instability of Human and Experimental Atherosclerotic Plaque |
| dc.creator.none.fl_str_mv |
González-López, Paula Ares-Carral, Carla López-Pastor, Andrea R. Infante-Menéndez, Jorge González Illanes, Tamara Vega de Ceniga, Melina Esparza, Leticia Beneit, Nuria Martín-Ventura, José Luis Escribano Illanes, Óscar Gómez Hernández, María De La Almudena |
| author |
González-López, Paula |
| author_facet |
González-López, Paula Ares-Carral, Carla López-Pastor, Andrea R. Infante-Menéndez, Jorge González Illanes, Tamara Vega de Ceniga, Melina Esparza, Leticia Beneit, Nuria Martín-Ventura, José Luis Escribano Illanes, Óscar Gómez Hernández, María De La Almudena |
| author_role |
author |
| author2 |
Ares-Carral, Carla López-Pastor, Andrea R. Infante-Menéndez, Jorge González Illanes, Tamara Vega de Ceniga, Melina Esparza, Leticia Beneit, Nuria Martín-Ventura, José Luis Escribano Illanes, Óscar Gómez Hernández, María De La Almudena |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidad Complutense de Madrid |
| dc.subject.none.fl_str_mv |
577.2 atherosclerosis miR-155-5p miR-143-3p unstable plaque apoptosis AKT IGF-IIR Biología molecular (Farmacia) Bioquímica (Farmacia) 2302 Bioquímica 2415 Biología Molecular |
| topic |
577.2 atherosclerosis miR-155-5p miR-143-3p unstable plaque apoptosis AKT IGF-IIR Biología molecular (Farmacia) Bioquímica (Farmacia) 2302 Bioquímica 2415 Biología Molecular |
| description |
(1) Background: Cardiovascular diseases (CVDs) are the main cause of death in developed countries, being atherosclerosis, a recurring process underlying their apparition. MicroRNAs (miRNAs) modulate the expression of their targets and have emerged as key players in CVDs; (2) Methods: 18 miRNAs were selected (Pubmed and GEO database) for their possible role in promoting atherosclerosis and were analysed by RT-qPCR in the aorta from apolipoprotein E-deficient (ApoE−/−) mice. Afterwards, the altered miRNAs in the aorta from 18 weeks-ApoE−/− mice were studied in human aortic and carotid samples; (3) Results: miR-155-5p was overexpressed and miR-143-3p was downregulated in mouse and human atherosclerotic lesions. In addition, a significant decrease in protein kinase B (AKT), target of miR-155-5p, and an increase in insulin-like growth factor type II receptor (IGF-IIR), target of miR-143-3p, were noted in aortic roots from ApoE−/− mice and in carotid plaques from patients with advanced carotid atherosclerosis (ACA). Finally, the overexpression of miR-155-5p reduced AKT levels and its phosphorylation in vascular smooth muscle cells, while miR-143-3p overexpression decreased IGF-IIR reducing apoptosis in vascular cells; (4) Conclusions: Our results suggest that miR-155-5p and miR-143-3p may be implicated in insulin resistance and plaque instability by the modulation of their targets AKT and IGF-IIR, contributing to the progression of atherosclerosis. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2022-09-06 2022 2022-09-06 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/20.500.14352/93718 |
| url |
https://hdl.handle.net/20.500.14352/93718 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
RTI-2018-095098-B100 Not available Not available PID2021-123076OB-I00 Not available Not available PR75 18-21572 Not available AENC1 22-29754 Not available |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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reponame:Docta Complutense instname:Universidad Complutense de Madrid (UCM) |
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Universidad Complutense de Madrid (UCM) |
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Docta Complutense |
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