Antimicrobial peptide KSL-W and analogues: Promising agents to control plant diseases

Recent strong restrictions on the use of pesticides has prompted the search for safer alternatives, being antimicrobial peptides promising candidates. Herein, with the aim of identifying new agents, 15 peptides reported as plant defense elicitors, promiscuous, multifunctional or antimicrobial were s...

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Autores: Camó Marín, Cristina, Bonaterra i Carreras, Anna, Badosa Romañó, Esther, Baró Sabé, Aina, Montesinos Barreda, Laura, Montesinos Seguí, Emilio, Planas i Grabuleda, Marta, Feliu Soley, Lidia
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/16210
Acesso em linha:http://hdl.handle.net/10256/16210
Access Level:acceso abierto
Palavra-chave:Antibiòtics pèptids
Peptide antibiotics
Plantes -- Malalties bacterianes
Bacterial diseases of plants
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spelling Antimicrobial peptide KSL-W and analogues: Promising agents to control plant diseasesCamó Marín, CristinaBonaterra i Carreras, AnnaBadosa Romañó, EstherBaró Sabé, AinaMontesinos Barreda, LauraMontesinos Seguí, EmilioPlanas i Grabuleda, MartaFeliu Soley, LidiaAntibiòtics pèptidsPeptide antibioticsPlantes -- Malalties bacterianesBacterial diseases of plantsRecent strong restrictions on the use of pesticides has prompted the search for safer alternatives, being antimicrobial peptides promising candidates. Herein, with the aim of identifying new agents, 15 peptides reported as plant defense elicitors, promiscuous, multifunctional or antimicrobial were selected and tested against six plant pathogenic bacteria of economic importance. Within this set, KSL-W (KKVVFWVKFK-NH2) displayed high antibacterial activity against all the tested pathogens, low hemolysis and low phytotoxicity in tobacco leaves. This peptide was taken as a lead and 49 analogues were designed and synthesized, including N-terminal deletion sequences, peptides incorporating a d-amino acid and lipopeptides. The screening of these sequences revealed that a nine amino acid length was the minimum for activity. The presence of a d-amino acid significantly decreased the hemolysis and endowed KSL-W with the capacity to induce the expression of defense-related genes in tomato plants. The incorporation of an acyl chain led to sequences with high activity against Xanthomonas strains, low hemolysis and phytotoxicity. Therefore, this study demonstrates that KSL-W constitutes an excellent candidate as new agent to control plant diseases and can be considered as a lead to develop derivatives with multifunctional properties, including antimicrobial and plant defense elicitationC.C. was the recipient of a predoctoral fellowship from the MINECO of Spain. This work was financed by grants AGL2012-39880-C02-02, AGL2015-69876-C2-2-R (MINECO/FEDER, EU) and MPCUdG2016/038. The authors acknowledge the Serveis Tècnics de Recerca of the University of Girona for the mass spectrometry analysisElsevierMinisterio de Ciencia e Innovación (Espanya)Ministerio de Economía y Competitividad (Espanya)2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionpeer-reviewed11 p.application/pdfhttp://hdl.handle.net/10256/16210© Peptides, 2019, vol. 112, p. 85-95Articles publicats (D-Q)Camó Marin, Cristina Bonaterra i Carreras, Anna Badosa Romañó, Esther Baró, Aina Montesinos Barreda, Laura Montesinos Seguí, Emilio Planas i Grabuleda, Marta Feliu Soley, Lídia 2019 Antimicrobial peptide KSL-W and analogues: Promising agents to control plant diseases Peptides 112 85 95reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.peptides.2018.11.009info:eu-repo/semantics/altIdentifier/issn/0196-9781info:eu-repo/grantAgreement/MINECO//AGL2012-39880-C02-02info:eu-repo/grantAgreement/MINECO//AGL2015-69876-C2-2-RTots els drets reservatsinfo:eu-repo/semantics/openAccessoai:recercat.cat:10256/162102026-05-29T05:05:01Z
dc.title.none.fl_str_mv Antimicrobial peptide KSL-W and analogues: Promising agents to control plant diseases
title Antimicrobial peptide KSL-W and analogues: Promising agents to control plant diseases
spellingShingle Antimicrobial peptide KSL-W and analogues: Promising agents to control plant diseases
Camó Marín, Cristina
Antibiòtics pèptids
Peptide antibiotics
Plantes -- Malalties bacterianes
Bacterial diseases of plants
title_short Antimicrobial peptide KSL-W and analogues: Promising agents to control plant diseases
title_full Antimicrobial peptide KSL-W and analogues: Promising agents to control plant diseases
title_fullStr Antimicrobial peptide KSL-W and analogues: Promising agents to control plant diseases
title_full_unstemmed Antimicrobial peptide KSL-W and analogues: Promising agents to control plant diseases
title_sort Antimicrobial peptide KSL-W and analogues: Promising agents to control plant diseases
dc.creator.none.fl_str_mv Camó Marín, Cristina
Bonaterra i Carreras, Anna
Badosa Romañó, Esther
Baró Sabé, Aina
Montesinos Barreda, Laura
Montesinos Seguí, Emilio
Planas i Grabuleda, Marta
Feliu Soley, Lidia
author Camó Marín, Cristina
author_facet Camó Marín, Cristina
Bonaterra i Carreras, Anna
Badosa Romañó, Esther
Baró Sabé, Aina
Montesinos Barreda, Laura
Montesinos Seguí, Emilio
Planas i Grabuleda, Marta
Feliu Soley, Lidia
author_role author
author2 Bonaterra i Carreras, Anna
Badosa Romañó, Esther
Baró Sabé, Aina
Montesinos Barreda, Laura
Montesinos Seguí, Emilio
Planas i Grabuleda, Marta
Feliu Soley, Lidia
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (Espanya)
Ministerio de Economía y Competitividad (Espanya)
dc.subject.none.fl_str_mv Antibiòtics pèptids
Peptide antibiotics
Plantes -- Malalties bacterianes
Bacterial diseases of plants
topic Antibiòtics pèptids
Peptide antibiotics
Plantes -- Malalties bacterianes
Bacterial diseases of plants
description Recent strong restrictions on the use of pesticides has prompted the search for safer alternatives, being antimicrobial peptides promising candidates. Herein, with the aim of identifying new agents, 15 peptides reported as plant defense elicitors, promiscuous, multifunctional or antimicrobial were selected and tested against six plant pathogenic bacteria of economic importance. Within this set, KSL-W (KKVVFWVKFK-NH2) displayed high antibacterial activity against all the tested pathogens, low hemolysis and low phytotoxicity in tobacco leaves. This peptide was taken as a lead and 49 analogues were designed and synthesized, including N-terminal deletion sequences, peptides incorporating a d-amino acid and lipopeptides. The screening of these sequences revealed that a nine amino acid length was the minimum for activity. The presence of a d-amino acid significantly decreased the hemolysis and endowed KSL-W with the capacity to induce the expression of defense-related genes in tomato plants. The incorporation of an acyl chain led to sequences with high activity against Xanthomonas strains, low hemolysis and phytotoxicity. Therefore, this study demonstrates that KSL-W constitutes an excellent candidate as new agent to control plant diseases and can be considered as a lead to develop derivatives with multifunctional properties, including antimicrobial and plant defense elicitation
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
peer-reviewed
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/16210
url http://hdl.handle.net/10256/16210
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.peptides.2018.11.009
info:eu-repo/semantics/altIdentifier/issn/0196-9781
info:eu-repo/grantAgreement/MINECO//AGL2012-39880-C02-02
info:eu-repo/grantAgreement/MINECO//AGL2015-69876-C2-2-R
dc.rights.none.fl_str_mv Tots els drets reservats
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Tots els drets reservats
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv © Peptides, 2019, vol. 112, p. 85-95
Articles publicats (D-Q)
Camó Marin, Cristina Bonaterra i Carreras, Anna Badosa Romañó, Esther Baró, Aina Montesinos Barreda, Laura Montesinos Seguí, Emilio Planas i Grabuleda, Marta Feliu Soley, Lídia 2019 Antimicrobial peptide KSL-W and analogues: Promising agents to control plant diseases Peptides 112 85 95
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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