Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep

17 páginas, 9 figuras, 1 tabla.

Detalles Bibliográficos
Autores: Criado, Miguel, Reyes, Luis E, García Marín, Juan Francisco, Gutiérrez-Expósito, Daniel, Zapico, David, Espinosa Cerrato, José, Pérez, Valentín
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/362828
Acceso en línea:http://hdl.handle.net/10261/362828
https://api.elsevier.com/content/abstract/scopus_id/85184698024
Access Level:acceso abierto
Palabra clave:Gudair
Silirum
Adjuvants
Granuloma
Paratuberculosis
Vaccine injection-site
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
adjuvants
granuloma
paratuberculosis
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oai_identifier_str oai:digital.csic.es:10261/362828
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep
title Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep
spellingShingle Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep
Criado, Miguel
Gudair
Silirum
Adjuvants
Granuloma
Paratuberculosis
Vaccine injection-site
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
adjuvants
granuloma
paratuberculosis
title_short Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep
title_full Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep
title_fullStr Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep
title_full_unstemmed Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep
title_sort Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep
dc.creator.none.fl_str_mv Criado, Miguel
Reyes, Luis E
García Marín, Juan Francisco
Gutiérrez-Expósito, Daniel
Zapico, David
Espinosa Cerrato, José
Pérez, Valentín
author Criado, Miguel
author_facet Criado, Miguel
Reyes, Luis E
García Marín, Juan Francisco
Gutiérrez-Expósito, Daniel
Zapico, David
Espinosa Cerrato, José
Pérez, Valentín
author_role author
author2 Reyes, Luis E
García Marín, Juan Francisco
Gutiérrez-Expósito, Daniel
Zapico, David
Espinosa Cerrato, José
Pérez, Valentín
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
European Commission
Universidad de León
Gutiérrez Expósito, Daniel [0000-0002-7683-623X]
Pérez Pérez, Valentín [0000-0003-0075-1587]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Gudair
Silirum
Adjuvants
Granuloma
Paratuberculosis
Vaccine injection-site
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
adjuvants
granuloma
paratuberculosis
topic Gudair
Silirum
Adjuvants
Granuloma
Paratuberculosis
Vaccine injection-site
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
adjuvants
granuloma
paratuberculosis
description 17 páginas, 9 figuras, 1 tabla.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/362828
https://api.elsevier.com/content/abstract/scopus_id/85184698024
url http://hdl.handle.net/10261/362828
https://api.elsevier.com/content/abstract/scopus_id/85184698024
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
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info:eu-repo/grantAgreement/AEI//PRE2019– 087309
info:eu-repo/grantAgreement/AEI//RTI2018–099496-BI00
info:eu-repo/grantAgreement/AEI//PID2021–125807OB-C21
Frontiers in veterinary science
http://dx.doi.org/doi.org/10.3389/fvets.2024.1284902
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheepCriado, MiguelReyes, Luis EGarcía Marín, Juan FranciscoGutiérrez-Expósito, DanielZapico, DavidEspinosa Cerrato, JoséPérez, ValentínGudairSilirumAdjuvantsGranulomaParatuberculosisVaccine injection-sitehttp://metadata.un.org/sdg/12Ensure sustainable consumption and production patternsadjuvantsgranulomaparatuberculosis17 páginas, 9 figuras, 1 tabla.Vaccination is the most effective tool for paratuberculosis control. Currently, available vaccines prevent the progression of clinical disease in most animals but do not fully protect them against infection and induce the formation of an injection site granuloma. The precise mechanisms that operate in response to vaccination and granuloma development, as well as the effect that adjuvants could trigger, have not been fully investigated. Therefore, this study aimed to investigate the injection site granulomas induced by two inactivated paratuberculosis vaccines, which differ in the adjuvant employed. Two groups of 45-day-old lambs were immunized with two commercially available vaccines-one (n = 4) with Gudair® and the other (n = 4) with Silirum®. A third group (n = 4) was not vaccinated and served as control. The peripheral humoral response was assessed throughout the study by a commercial anti-Mycobacterium avium subspecies paratuberculosis (Map) antibody indirect ELISA, and the cellular immune response was assessed similarly by the IFN-γ release and comparative intradermal tests. The injection site granulomas were measured during the experiment and sampled at 75 days post-vaccination (dpv) when the animals were euthanized. The tissue damage, antigen and adjuvant distribution, and the presence and amount of immune cells were then determined and assessed by immunohistochemical methods. Antibodies against Map antigens; a general macrophage marker (Iba1), M1 (iNOS), and M2 (CD204) macrophages; T (CD3), B (CD20), and γδ T lymphocytes, proteins MHC-II and NRAMP1, and cytokines IL-4, IL-10, TNF, and IFN-γ were employed. Silirum® elicited a stronger peripheral cellular immune response than Gudair®, while the latter induced larger granulomas and more tissue damage at the site of injection. Additionally, adjuvant and Map antigen distribution throughout the granulomatous inflammatory infiltrate, as well as the NRAMP1 cell expression, which is linked to antigen phagocytosis, were highly irregular. In Silirum® induced granulomas, a higher number of MHC-II and TNF-expressing cells and a lower number of M2 macrophages suggested an improved antigen presentation, which could be due to the better antigen distribution and reduced tissue damage induced by this vaccine.The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was funded by the national project RTI2018–099496-BI00 and PID2021–125807OB-C21, from the “Agencia Estatal de Investigación”, of the Spanish Ministry of Science and Innovation. MC acknowledges the receipt of a pre-doctoral grant (PRE2019– 087309) from MCIN/AEI/ 10.13039/501100011033 and FSE “El FSE invierte en tu futuro” and DZ of a predoctoral contract of the University of León. Zendal (Porriño, Spain), supplied Gudair R and Silirum R vaccines.Peer reviewedFrontiers MediaAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)European CommissionUniversidad de LeónGutiérrez Expósito, Daniel [0000-0002-7683-623X]Pérez Pérez, Valentín [0000-0003-0075-1587]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/362828https://api.elsevier.com/content/abstract/scopus_id/85184698024reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//PRE2019– 087309info:eu-repo/grantAgreement/AEI//RTI2018–099496-BI00info:eu-repo/grantAgreement/AEI//PID2021–125807OB-C21Frontiers in veterinary sciencehttp://dx.doi.org/doi.org/10.3389/fvets.2024.1284902Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3628282026-05-22T06:33:51Z
score 15,81155

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