Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARSCoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021

Since its first identification in the United Kingdom in late 2020, the highly transmissible B.1.1.7 variant of SARS-CoV-2 has become dominant in several countries raising great concern. We developed a duplex real-time RT-qPCR assay to detect, discriminate, and quantitate SARS-CoV-2 variants containi...

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Detalhes bibliográficos
Autores: Carcereny, Albert, Martínez-Velázquez, Adán, Bosch, Albert, Allende, Anna, Truchado, Pilar, Cascales, Jenifer, Romalde, Jesús, Lois, Marta, Polo, David, Sánchez, Gloria, Pérez-Cataluña, Alba, Díaz-Reolid, Azahara, Antón, Andrés, Gregor, Josep, Garcia-Cehic, Damir, Quer, Josep, Palau, Margarita, González Ruano, Cristina, Pintó Solé, Rosa María, Guix, Susana
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2021
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/193482
Acesso em linha:https://hdl.handle.net/2445/193482
Access Level:acceso abierto
Palavra-chave:SARS-CoV-2
COVID-19
Epidemiologia
Epidemiology
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spelling Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARSCoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021Carcereny, AlbertMartínez-Velázquez, AdánBosch, AlbertAllende, AnnaTruchado, PilarCascales, JeniferRomalde, JesúsLois, MartaPolo, DavidSánchez, GloriaPérez-Cataluña, AlbaDíaz-Reolid, AzaharaAntón, AndrésGregor, JosepGarcia-Cehic, DamirQuer, JosepPalau, MargaritaGonzález Ruano, CristinaPintó Solé, Rosa MaríaGuix, SusanaSARS-CoV-2COVID-19EpidemiologiaSARS-CoV-2COVID-19EpidemiologySince its first identification in the United Kingdom in late 2020, the highly transmissible B.1.1.7 variant of SARS-CoV-2 has become dominant in several countries raising great concern. We developed a duplex real-time RT-qPCR assay to detect, discriminate, and quantitate SARS-CoV-2 variants containing one of its mutation signatures, the ΔHV69/70 deletion, and used it to trace the community circulation of the B.1.1.7 variant in Spain through the Spanish National SARS-CoV-2 Wastewater Surveillance System (VATar COVID-19). The B.1.1.7 variant was detected earlier than clinical epidemiological reporting by the local authorities, first in the southern city of Málaga (Andalucía) in week 20_52 (year_week), and multiple introductions during Christmas holidays were inferred in different parts of the country. Wastewater-based B.1.1.7 tracking showed a good correlation with clinical data and provided information at the local level. Data from wastewater treatment plants, which reached B.1.1.7 prevalences higher than 90% for ≥2 consecutive weeks showed that 8.1 ± 2.0 weeks were required for B.1.1.7 to become dominant. The study highlights the applicability of RT-qPCR-based strategies to track specific mutations of variants of concern as soon as they are identified by clinical sequencing and their integration into existing wastewater surveillance programs, as a cost-effective approach to complement clinical testing during the COVID-19 pandemic.American Chemical Society2023202320212023info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion37 p.application/pdfhttps://hdl.handle.net/2445/193482Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1021/acs.est.1c03589Environmental Science & Technology, 2021, vol. 55, num. 17, p. 11756-11766https://doi.org/10.1021/acs.est.1c03589(c) American Chemical Society , 2021info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1934822026-05-29T05:05:01Z
dc.title.none.fl_str_mv Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARSCoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021
title Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARSCoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021
spellingShingle Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARSCoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021
Carcereny, Albert
SARS-CoV-2
COVID-19
Epidemiologia
SARS-CoV-2
COVID-19
Epidemiology
title_short Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARSCoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021
title_full Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARSCoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021
title_fullStr Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARSCoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021
title_full_unstemmed Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARSCoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021
title_sort Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARSCoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021
dc.creator.none.fl_str_mv Carcereny, Albert
Martínez-Velázquez, Adán
Bosch, Albert
Allende, Anna
Truchado, Pilar
Cascales, Jenifer
Romalde, Jesús
Lois, Marta
Polo, David
Sánchez, Gloria
Pérez-Cataluña, Alba
Díaz-Reolid, Azahara
Antón, Andrés
Gregor, Josep
Garcia-Cehic, Damir
Quer, Josep
Palau, Margarita
González Ruano, Cristina
Pintó Solé, Rosa María
Guix, Susana
author Carcereny, Albert
author_facet Carcereny, Albert
Martínez-Velázquez, Adán
Bosch, Albert
Allende, Anna
Truchado, Pilar
Cascales, Jenifer
Romalde, Jesús
Lois, Marta
Polo, David
Sánchez, Gloria
Pérez-Cataluña, Alba
Díaz-Reolid, Azahara
Antón, Andrés
Gregor, Josep
Garcia-Cehic, Damir
Quer, Josep
Palau, Margarita
González Ruano, Cristina
Pintó Solé, Rosa María
Guix, Susana
author_role author
author2 Martínez-Velázquez, Adán
Bosch, Albert
Allende, Anna
Truchado, Pilar
Cascales, Jenifer
Romalde, Jesús
Lois, Marta
Polo, David
Sánchez, Gloria
Pérez-Cataluña, Alba
Díaz-Reolid, Azahara
Antón, Andrés
Gregor, Josep
Garcia-Cehic, Damir
Quer, Josep
Palau, Margarita
González Ruano, Cristina
Pintó Solé, Rosa María
Guix, Susana
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SARS-CoV-2
COVID-19
Epidemiologia
SARS-CoV-2
COVID-19
Epidemiology
topic SARS-CoV-2
COVID-19
Epidemiologia
SARS-CoV-2
COVID-19
Epidemiology
description Since its first identification in the United Kingdom in late 2020, the highly transmissible B.1.1.7 variant of SARS-CoV-2 has become dominant in several countries raising great concern. We developed a duplex real-time RT-qPCR assay to detect, discriminate, and quantitate SARS-CoV-2 variants containing one of its mutation signatures, the ΔHV69/70 deletion, and used it to trace the community circulation of the B.1.1.7 variant in Spain through the Spanish National SARS-CoV-2 Wastewater Surveillance System (VATar COVID-19). The B.1.1.7 variant was detected earlier than clinical epidemiological reporting by the local authorities, first in the southern city of Málaga (Andalucía) in week 20_52 (year_week), and multiple introductions during Christmas holidays were inferred in different parts of the country. Wastewater-based B.1.1.7 tracking showed a good correlation with clinical data and provided information at the local level. Data from wastewater treatment plants, which reached B.1.1.7 prevalences higher than 90% for ≥2 consecutive weeks showed that 8.1 ± 2.0 weeks were required for B.1.1.7 to become dominant. The study highlights the applicability of RT-qPCR-based strategies to track specific mutations of variants of concern as soon as they are identified by clinical sequencing and their integration into existing wastewater surveillance programs, as a cost-effective approach to complement clinical testing during the COVID-19 pandemic.
publishDate 2021
dc.date.none.fl_str_mv 2021
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/193482
url https://hdl.handle.net/2445/193482
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1021/acs.est.1c03589
Environmental Science & Technology, 2021, vol. 55, num. 17, p. 11756-11766
https://doi.org/10.1021/acs.est.1c03589
dc.rights.none.fl_str_mv (c) American Chemical Society , 2021
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Chemical Society , 2021
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 37 p.
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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