Integrated brain and testis transcriptomic analyses reveal the benefits of sandy environmental enrichment in Senegalese sole male (Solea senegalensis) reproduction

This study investigates the effects of physical enrichment (sand substrate) on the gonadal development and transcriptomics of juvenile Senegalese sole (Solea senegalensis). A total of 1500 fish were distributed into six outdoor fiberglass tanks, three of which included a 2 cm layer of sand to simula...

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Detalles Bibliográficos
Autores: Fatsini, Elvira, Ramos-Júdez, Sandra, Chauvigné, François, Cerdà, Joan, Oliveira, Catarina, Cabrita, Elsa
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/4919
Acceso en línea:https://hdl.handle.net/20.500.12327/4919
https://doi.org/10.1016/j.aquaculture.2025.743468
Access Level:acceso abierto
Palabra clave:637
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spelling Integrated brain and testis transcriptomic analyses reveal the benefits of sandy environmental enrichment in Senegalese sole male (Solea senegalensis) reproductionFatsini, ElviraRamos-Júdez, SandraChauvigné, FrançoisCerdà, JoanOliveira, CatarinaCabrita, Elsa637This study investigates the effects of physical enrichment (sand substrate) on the gonadal development and transcriptomics of juvenile Senegalese sole (Solea senegalensis). A total of 1500 fish were distributed into six outdoor fiberglass tanks, three of which included a 2 cm layer of sand to simulate physical enrichment. Throughout a two-year study, the fish were sampled at four intervals, corresponding to developmental stages ranging from juvenile, pre-pubertal to adult. Blood, testis, and brain tissues were collected at each sampling point to analyse hormonal profiles (estradiol, testosterone, follicle-stimulating and luteinizing hormones) and gene expression. Histological analysis of testicular tissue was used to assess the germ cell development stages, while RNA sequencing (RNA-seq) was performed to evaluate the impact of physical enrichment on transcriptomic profiles in both the brain and testes. Sandy environmental enrichment is associated with increased testosterone and luteinizing hormone levels. Bioinformatics analysis identified differentially expressed genes (DETs) associated with reproduction, immune response, and metabolism. GO and KEGG enrichment analysis demonstrated that important terms and pathways associated with the process of spermatogenesis, cognition and metabolism where differentially enriched between males maintained in sand and fiberglass. These results provided new insights into how environmental enrichment influences reproductive physiology and molecular mechanisms in cultured Senegalese sole from very early stages.info:eu-repo/semantics/publishedVersionElsevierProducció AnimalAqüicultura2025info:eu-repo/semantics/article15https://hdl.handle.net/20.500.12327/4919https://doi.org/10.1016/j.aquaculture.2025.743468reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésAquacultureAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/49192026-05-29T05:05:01Z
dc.title.none.fl_str_mv Integrated brain and testis transcriptomic analyses reveal the benefits of sandy environmental enrichment in Senegalese sole male (Solea senegalensis) reproduction
title Integrated brain and testis transcriptomic analyses reveal the benefits of sandy environmental enrichment in Senegalese sole male (Solea senegalensis) reproduction
spellingShingle Integrated brain and testis transcriptomic analyses reveal the benefits of sandy environmental enrichment in Senegalese sole male (Solea senegalensis) reproduction
Fatsini, Elvira
637
title_short Integrated brain and testis transcriptomic analyses reveal the benefits of sandy environmental enrichment in Senegalese sole male (Solea senegalensis) reproduction
title_full Integrated brain and testis transcriptomic analyses reveal the benefits of sandy environmental enrichment in Senegalese sole male (Solea senegalensis) reproduction
title_fullStr Integrated brain and testis transcriptomic analyses reveal the benefits of sandy environmental enrichment in Senegalese sole male (Solea senegalensis) reproduction
title_full_unstemmed Integrated brain and testis transcriptomic analyses reveal the benefits of sandy environmental enrichment in Senegalese sole male (Solea senegalensis) reproduction
title_sort Integrated brain and testis transcriptomic analyses reveal the benefits of sandy environmental enrichment in Senegalese sole male (Solea senegalensis) reproduction
dc.creator.none.fl_str_mv Fatsini, Elvira
Ramos-Júdez, Sandra
Chauvigné, François
Cerdà, Joan
Oliveira, Catarina
Cabrita, Elsa
author Fatsini, Elvira
author_facet Fatsini, Elvira
Ramos-Júdez, Sandra
Chauvigné, François
Cerdà, Joan
Oliveira, Catarina
Cabrita, Elsa
author_role author
author2 Ramos-Júdez, Sandra
Chauvigné, François
Cerdà, Joan
Oliveira, Catarina
Cabrita, Elsa
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Aqüicultura
dc.subject.none.fl_str_mv 637
topic 637
description This study investigates the effects of physical enrichment (sand substrate) on the gonadal development and transcriptomics of juvenile Senegalese sole (Solea senegalensis). A total of 1500 fish were distributed into six outdoor fiberglass tanks, three of which included a 2 cm layer of sand to simulate physical enrichment. Throughout a two-year study, the fish were sampled at four intervals, corresponding to developmental stages ranging from juvenile, pre-pubertal to adult. Blood, testis, and brain tissues were collected at each sampling point to analyse hormonal profiles (estradiol, testosterone, follicle-stimulating and luteinizing hormones) and gene expression. Histological analysis of testicular tissue was used to assess the germ cell development stages, while RNA sequencing (RNA-seq) was performed to evaluate the impact of physical enrichment on transcriptomic profiles in both the brain and testes. Sandy environmental enrichment is associated with increased testosterone and luteinizing hormone levels. Bioinformatics analysis identified differentially expressed genes (DETs) associated with reproduction, immune response, and metabolism. GO and KEGG enrichment analysis demonstrated that important terms and pathways associated with the process of spermatogenesis, cognition and metabolism where differentially enriched between males maintained in sand and fiberglass. These results provided new insights into how environmental enrichment influences reproductive physiology and molecular mechanisms in cultured Senegalese sole from very early stages.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12327/4919
https://doi.org/10.1016/j.aquaculture.2025.743468
url https://hdl.handle.net/20.500.12327/4919
https://doi.org/10.1016/j.aquaculture.2025.743468
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Aquaculture
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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