Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase
Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxa...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Universitat Pompeu Fabra |
| Repositorio: | Repositorio Digital de la UPF |
| OAI Identifier: | oai:repositori.upf.edu:10230/27997 |
| Acceso en línea: | http://hdl.handle.net/10230/27997 http://dx.doi.org/10.1111/pbi.12359 |
| Access Level: | acceso abierto |
| Palabra clave: | CDNA-AFLP CoA ligase Methyl jasmonate Beta-phenylalanine Taxol Taxus baccata Cell cultures |
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Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligaseRamírez Estrada, Karla Altabella, TeresaOnrubia Ibáñez, Miriam, 1982-Moyano Claramunt, ElisabetNotredame, CedricOsuna, LidiaVanden Bossche, RobinGoossens, AlainCusidó Vidal, Rosa M.Palazón Barandela, JavierCDNA-AFLPCoA ligaseMethyl jasmonateBeta-phenylalanineTaxolTaxus baccataCell culturesPlant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer compounds, several aspects of their biosynthesis remain unknown. In this work, a genomewide expression analysis of jasmonate-elicited Taxus baccata cell cultures by complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) indicated a correlation between an extensive elicitor-induced genetic reprogramming and increased taxane production in the targeted cultures. Subsequent in silico analysis allowed us to identify 15 genes with a jasmonate-induced differential expression as putative candidates for genes encoding enzymes involved in five unknown steps of taxane biosynthesis. Among them, the TB768 gene showed a strong homology, including a very similar predicted 3D structure, with other genes previously reported to encode acyl-CoA ligases, thus suggesting a role in the formation of the taxol lateral chain. Functional analysis confirmed that the TB768 gene encodes an acyl-CoA ligase that localizes to the cytoplasm and is able to convert β-phenylalanine, as well as coumaric acid, into their respective derivative CoA esters. β-phenylalanyl-CoA is attached to baccatin III in one of the last steps of the taxol biosynthetic pathway. The identification of this gene will contribute to the establishment of sustainable taxol production systems through metabolic engineering or synthetic biology approaches.This research has been supported by two grants from the Spanish MINECO (BIO2011-29856-C02-01 and AGL2013-43522-R) and a grant from Generalitat de Catalunya (2014SGR1434).Wiley201720172016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/27997http://dx.doi.org/10.1111/pbi.12359reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésPlant Biotechnology Journal. 2016;14(1):85-96info:eu-repo/grantAgreement/ES/3PN/BIO2011-29856-C02-01info:eu-repo/grantAgreement/ES/1PE/AGL2013-43522-R© Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd. All articles accepted from 1st January 2016 (date of flip) are published under the terms of the Creative Commons Attribution License. Articles accepted before this date were published under the agreement as stated in the final article.https://creativecommons.org/licenses/by/3.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/279972026-06-12T07:21:37Z |
| dc.title.none.fl_str_mv |
Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase |
| title |
Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase |
| spellingShingle |
Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase Ramírez Estrada, Karla CDNA-AFLP CoA ligase Methyl jasmonate Beta-phenylalanine Taxol Taxus baccata Cell cultures |
| title_short |
Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase |
| title_full |
Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase |
| title_fullStr |
Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase |
| title_full_unstemmed |
Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase |
| title_sort |
Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase |
| dc.creator.none.fl_str_mv |
Ramírez Estrada, Karla Altabella, Teresa Onrubia Ibáñez, Miriam, 1982- Moyano Claramunt, Elisabet Notredame, Cedric Osuna, Lidia Vanden Bossche, Robin Goossens, Alain Cusidó Vidal, Rosa M. Palazón Barandela, Javier |
| author |
Ramírez Estrada, Karla |
| author_facet |
Ramírez Estrada, Karla Altabella, Teresa Onrubia Ibáñez, Miriam, 1982- Moyano Claramunt, Elisabet Notredame, Cedric Osuna, Lidia Vanden Bossche, Robin Goossens, Alain Cusidó Vidal, Rosa M. Palazón Barandela, Javier |
| author_role |
author |
| author2 |
Altabella, Teresa Onrubia Ibáñez, Miriam, 1982- Moyano Claramunt, Elisabet Notredame, Cedric Osuna, Lidia Vanden Bossche, Robin Goossens, Alain Cusidó Vidal, Rosa M. Palazón Barandela, Javier |
| author2_role |
author author author author author author author author author |
| dc.subject.none.fl_str_mv |
CDNA-AFLP CoA ligase Methyl jasmonate Beta-phenylalanine Taxol Taxus baccata Cell cultures |
| topic |
CDNA-AFLP CoA ligase Methyl jasmonate Beta-phenylalanine Taxol Taxus baccata Cell cultures |
| description |
Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer compounds, several aspects of their biosynthesis remain unknown. In this work, a genomewide expression analysis of jasmonate-elicited Taxus baccata cell cultures by complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) indicated a correlation between an extensive elicitor-induced genetic reprogramming and increased taxane production in the targeted cultures. Subsequent in silico analysis allowed us to identify 15 genes with a jasmonate-induced differential expression as putative candidates for genes encoding enzymes involved in five unknown steps of taxane biosynthesis. Among them, the TB768 gene showed a strong homology, including a very similar predicted 3D structure, with other genes previously reported to encode acyl-CoA ligases, thus suggesting a role in the formation of the taxol lateral chain. Functional analysis confirmed that the TB768 gene encodes an acyl-CoA ligase that localizes to the cytoplasm and is able to convert β-phenylalanine, as well as coumaric acid, into their respective derivative CoA esters. β-phenylalanyl-CoA is attached to baccatin III in one of the last steps of the taxol biosynthetic pathway. The identification of this gene will contribute to the establishment of sustainable taxol production systems through metabolic engineering or synthetic biology approaches. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 2017 2017 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10230/27997 http://dx.doi.org/10.1111/pbi.12359 |
| url |
http://hdl.handle.net/10230/27997 http://dx.doi.org/10.1111/pbi.12359 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Plant Biotechnology Journal. 2016;14(1):85-96 info:eu-repo/grantAgreement/ES/3PN/BIO2011-29856-C02-01 info:eu-repo/grantAgreement/ES/1PE/AGL2013-43522-R |
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https://creativecommons.org/licenses/by/3.0/ info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/3.0/ |
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openAccess |
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application/pdf application/pdf |
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Wiley |
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Wiley |
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reponame:Repositorio Digital de la UPF instname:Universitat Pompeu Fabra |
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Universitat Pompeu Fabra |
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Repositorio Digital de la UPF |
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Repositorio Digital de la UPF |
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