Mercury speciation in edible seaweed by liquid chromatography - Inductively coupled plasma mass spectrometry after ionic imprinted polymer-solid phase extraction

In contrast to most of essential and heavy metals, mercury levels in seaweed are very low, and pre-concentration methods are required for an adequate total mercury determination and mercury speciation in this foodstuff. An ionic imprinted polymer-based solid phase extraction (on column) pre-concentr...

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Autores: Herbello-Hermelo, Paloma, Peña Vázquez, Elena María, Bermejo Barrera, Pilar, Moreda Piñeiro, Antonio, Jinadasa, Kamal K.
Tipo de documento: artigo
Data de publicação:2021
País:España
Recursos:Universidad de Santiago de Compostela (USC)
Repositório:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglês
OAI Identifier:oai:minerva.usc.gal:10347/32586
Acesso em linha:http://hdl.handle.net/10347/32586
Access Level:Acceso aberto
Palavra-chave:241707 Algología (ficología)
2301 Química analítica
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spelling Mercury speciation in edible seaweed by liquid chromatography - Inductively coupled plasma mass spectrometry after ionic imprinted polymer-solid phase extractionHerbello-Hermelo, PalomaPeña Vázquez, Elena MaríaBermejo Barrera, PilarMoreda Piñeiro, AntonioJinadasa, Kamal K.241707 Algología (ficología)2301 Química analíticaIn contrast to most of essential and heavy metals, mercury levels in seaweed are very low, and pre-concentration methods are required for an adequate total mercury determination and mercury speciation in this foodstuff. An ionic imprinted polymer-based solid phase extraction (on column) pre-concentration procedure has been optimized for mercury species enrichment before liquid chromatography hyphenated with inductively coupled plasma mass spectrometry determination. The polymer has been synthesized by the precipitation polymerization method and using a ternary pre-polymerization mixture containing the template (methylmercury), a non-vinylated monomer (phenobarbital), and a vinylated monomer (methacrylic acid). Factors affecting the adsorption/desorption of Hg species (extract pH, loading and elution flow rates, volume of eluent, etc.), and parameters such as breakthrough volume and reusability, were fully studied. Mercury species were first isolated from seaweed by ultrasound assisted extraction using a 0.1% (v/v) HCl, 0.12% (w/v) l-cysteine, 0.1% (v/v) mercaptoethanol solution. Under optimized conditions, the limits of detection were 0.007 and 0.02 μg kg−1 dw for methylmercury and Hg(II), respectively. The pre-concentration factor (volume of 10 mL of seaweed extract) was 50. Repeatability and reproducibility of the method were satisfactory with relative standard deviations lower than 16%. The proposed methodology was finally applied for the selective pre-concentration and determination of methylmercury and Hg (II) in a BCR-463 certified reference material and in several edible seaweeds.ElsevierUniversidade de Santiago de Compostela. Departamento de Química Analítica, Nutrición e Bromatoloxía20212021-03-0120212021-03-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10347/32586reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2CC BY-NC-ND 4.0http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/325862026-06-15T12:47:27Z
dc.title.none.fl_str_mv Mercury speciation in edible seaweed by liquid chromatography - Inductively coupled plasma mass spectrometry after ionic imprinted polymer-solid phase extraction
title Mercury speciation in edible seaweed by liquid chromatography - Inductively coupled plasma mass spectrometry after ionic imprinted polymer-solid phase extraction
spellingShingle Mercury speciation in edible seaweed by liquid chromatography - Inductively coupled plasma mass spectrometry after ionic imprinted polymer-solid phase extraction
Herbello-Hermelo, Paloma
241707 Algología (ficología)
2301 Química analítica
title_short Mercury speciation in edible seaweed by liquid chromatography - Inductively coupled plasma mass spectrometry after ionic imprinted polymer-solid phase extraction
title_full Mercury speciation in edible seaweed by liquid chromatography - Inductively coupled plasma mass spectrometry after ionic imprinted polymer-solid phase extraction
title_fullStr Mercury speciation in edible seaweed by liquid chromatography - Inductively coupled plasma mass spectrometry after ionic imprinted polymer-solid phase extraction
title_full_unstemmed Mercury speciation in edible seaweed by liquid chromatography - Inductively coupled plasma mass spectrometry after ionic imprinted polymer-solid phase extraction
title_sort Mercury speciation in edible seaweed by liquid chromatography - Inductively coupled plasma mass spectrometry after ionic imprinted polymer-solid phase extraction
dc.creator.none.fl_str_mv Herbello-Hermelo, Paloma
Peña Vázquez, Elena María
Bermejo Barrera, Pilar
Moreda Piñeiro, Antonio
Jinadasa, Kamal K.
author Herbello-Hermelo, Paloma
author_facet Herbello-Hermelo, Paloma
Peña Vázquez, Elena María
Bermejo Barrera, Pilar
Moreda Piñeiro, Antonio
Jinadasa, Kamal K.
author_role author
author2 Peña Vázquez, Elena María
Bermejo Barrera, Pilar
Moreda Piñeiro, Antonio
Jinadasa, Kamal K.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Departamento de Química Analítica, Nutrición e Bromatoloxía
dc.subject.none.fl_str_mv 241707 Algología (ficología)
2301 Química analítica
topic 241707 Algología (ficología)
2301 Química analítica
description In contrast to most of essential and heavy metals, mercury levels in seaweed are very low, and pre-concentration methods are required for an adequate total mercury determination and mercury speciation in this foodstuff. An ionic imprinted polymer-based solid phase extraction (on column) pre-concentration procedure has been optimized for mercury species enrichment before liquid chromatography hyphenated with inductively coupled plasma mass spectrometry determination. The polymer has been synthesized by the precipitation polymerization method and using a ternary pre-polymerization mixture containing the template (methylmercury), a non-vinylated monomer (phenobarbital), and a vinylated monomer (methacrylic acid). Factors affecting the adsorption/desorption of Hg species (extract pH, loading and elution flow rates, volume of eluent, etc.), and parameters such as breakthrough volume and reusability, were fully studied. Mercury species were first isolated from seaweed by ultrasound assisted extraction using a 0.1% (v/v) HCl, 0.12% (w/v) l-cysteine, 0.1% (v/v) mercaptoethanol solution. Under optimized conditions, the limits of detection were 0.007 and 0.02 μg kg−1 dw for methylmercury and Hg(II), respectively. The pre-concentration factor (volume of 10 mL of seaweed extract) was 50. Repeatability and reproducibility of the method were satisfactory with relative standard deviations lower than 16%. The proposed methodology was finally applied for the selective pre-concentration and determination of methylmercury and Hg (II) in a BCR-463 certified reference material and in several edible seaweeds.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-03-01
2021
2021-03-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10347/32586
url http://hdl.handle.net/10347/32586
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
CC BY-NC-ND 4.0
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
CC BY-NC-ND 4.0
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
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