Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner
[EN] Copper (Cu) deficiency compromises plant growth and limits crop productivity. Plants respond to Cu scarcity by activating the expression of several miRNAs, known as Cu-miRNAs, which degrade mRNAs from various cuproproteins to conserve Cu. Cu-miRNAs, like most plant miRNAs, associate with ARGONA...
| Autores: | , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/226363 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/226363 |
| Access Level: | acceso abierto |
| Palabra clave: | AGO1 AP-MS Arabidopsis thaliana Copper FeSOD1 MiRNA TST |
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Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner |
| title |
Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner |
| spellingShingle |
Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner Tomassi, Ariel H. AGO1 AP-MS Arabidopsis thaliana Copper FeSOD1 MiRNA TST |
| title_short |
Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner |
| title_full |
Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner |
| title_fullStr |
Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner |
| title_full_unstemmed |
Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner |
| title_sort |
Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent manner |
| dc.creator.none.fl_str_mv |
Tomassi, Ariel H. Perea-García, Ana Rodrigo, Guillermo Sánchez-Vicente, Javier Cisneros, Adriana E. Olmos, Marta Andrés-Bordería, Amparo López-Dolz, Lucio Peñarrubia, Lola Daròs, José-Antonio|||0000-0002-6535-2889 CARBONELL, ALBERTO|||0000-0001-5628-6632 |
| author |
Tomassi, Ariel H. |
| author_facet |
Tomassi, Ariel H. Perea-García, Ana Rodrigo, Guillermo Sánchez-Vicente, Javier Cisneros, Adriana E. Olmos, Marta Andrés-Bordería, Amparo López-Dolz, Lucio Peñarrubia, Lola Daròs, José-Antonio|||0000-0002-6535-2889 CARBONELL, ALBERTO|||0000-0001-5628-6632 |
| author_role |
author |
| author2 |
Perea-García, Ana Rodrigo, Guillermo Sánchez-Vicente, Javier Cisneros, Adriana E. Olmos, Marta Andrés-Bordería, Amparo López-Dolz, Lucio Peñarrubia, Lola Daròs, José-Antonio|||0000-0002-6535-2889 CARBONELL, ALBERTO|||0000-0001-5628-6632 |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto Universitario Mixto de Biología Molecular y Celular de Plantas Generalitat Valenciana Agencia Estatal de Investigación Ministerio de Ciencia e Innovación Ministerio de Ciencia, Innovación y Universidades Repositorio Institucional de la Universitat Politècnica de València Riunet |
| dc.subject.none.fl_str_mv |
AGO1 AP-MS Arabidopsis thaliana Copper FeSOD1 MiRNA TST |
| topic |
AGO1 AP-MS Arabidopsis thaliana Copper FeSOD1 MiRNA TST |
| description |
[EN] Copper (Cu) deficiency compromises plant growth and limits crop productivity. Plants respond to Cu scarcity by activating the expression of several miRNAs, known as Cu-miRNAs, which degrade mRNAs from various cuproproteins to conserve Cu. Cu-miRNAs, like most plant miRNAs, associate with ARGONAUTE 1 (AGO1), the primary effector protein of miRNA-mediated gene silencing pathways, whose function is typically modulated by interacting proteins acting as cofactors. However, how AGO1 is regulated and functions under Cu deficiency remains unknown. Here, we searched for AGO1 interactors in Arabidopsis thaliana plants expressing a functional AGO1 protein tagged with the Twin-Strep-tag (TST) polypeptide, grown under Cu-sufficient or Cu-deficient conditions. TST-AGO1 complexes were affinity purified, and proteins were identified using tandem MS. Interestingly, the iron superoxide dismutase 1 (FeSOD1) encoded by FSD1 was enriched in TST-AGO1 complexes purified from plants grown under Cu deficiency. Moreover, fsd1-2 mutant plants showed reduced levels of AGO1 compared with wild-type plants under Cu sufficiency, while both Cu-miRNA-specific and general AGO1 target mRNAs accumulated to higher levels in fsd1-2 plants under both Cu-deficient and Cu-sufficient conditions compared with wild-type plants. These findings suggest that FeSOD1 is essential for proper AGO1 function, and that its superoxide dismutase activity, which mitigates oxidative stress, enhances AGO1 stability, particularly under Cu deficiency.; AGO1 is essential for Cu-deficiency responses but is sensitive to oxidative stress. FeSOD1 interacts with AGO1 and protects it from superoxide radical-induced degradation, thereby preserving miRNA-mediated gene silencing pathways. |
| publishDate |
2025 |
| dc.date.none.fl_str_mv |
2025 2025-07-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://riunet.upv.es/handle/10251/226363 |
| url |
https://riunet.upv.es/handle/10251/226363 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 BIO2017-83184-R VIRUS DE PLANTAS: PATOGENOS Y TAMBIEN VECTORES PARA LA PRODUCCION DE PROTEINAS, METABOLITOS, RNAS Y NANOPARTICULAS Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-114691RB-I00 BIOTECNOLOGIA DE VIRUS DE PLANTAS: VECTORES VIRALES Y ESTRATEGIAS DE RESISTENCIA Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-116940RB-I00 MECANISMOS DE ADAPTACION A CONDICIONES NO OPTIMAS DE COBRE Y HIERRO EN LEVADURAS Y PLANTAS Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-095118-A-I00 COMPLEJOS ARGONAUTA1 DE PLANTAS: IDENTIFICACION DE SUS COMPONENTES PROTEICOS Y DE RNA, Y AJUSTE FINO DEL SILENCIAMIENTO MEDIANTE SU PROGRAMACION POR PEQUEÑOS RNAS ARTIFICIALES Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2021-122186OB-I00 PEQUEÑOS RNAS ARTIFICIALES: RNAI DE ULTIMA GENERACION PARA LA MEJORA DE CULTIVOS (ART-SRNAI) Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2021-127671NB-I00 INGENIERIA DE CIRCUITOS GENETICOS CON CABLEADO LUMINICO Y QUIMICO Generalitat Valenciana https://doi.org/10.13039/501100003359 CIPROM%2F2022%2F21 Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 RYC-2017-21648 Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 PRE2019-088439 |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Reconocimiento (by) http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 Reconocimiento (by) http://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Oxford University Press |
| publisher.none.fl_str_mv |
Oxford University Press |
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reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname:Universitat Politècnica de València (UPV) |
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Universitat Politècnica de València (UPV) |
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RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
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RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
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1869418020810522624 |
| spelling |
Arabidopsis thaliana iron superoxide dismutase FeSOD1 protects ARGONAUTE 1 in a copper-dependent mannerTomassi, Ariel H.Perea-García, AnaRodrigo, GuillermoSánchez-Vicente, JavierCisneros, Adriana E.Olmos, MartaAndrés-Bordería, AmparoLópez-Dolz, LucioPeñarrubia, LolaDaròs, José-Antonio|||0000-0002-6535-2889CARBONELL, ALBERTO|||0000-0001-5628-6632AGO1AP-MSArabidopsis thalianaCopperFeSOD1MiRNATST[EN] Copper (Cu) deficiency compromises plant growth and limits crop productivity. Plants respond to Cu scarcity by activating the expression of several miRNAs, known as Cu-miRNAs, which degrade mRNAs from various cuproproteins to conserve Cu. Cu-miRNAs, like most plant miRNAs, associate with ARGONAUTE 1 (AGO1), the primary effector protein of miRNA-mediated gene silencing pathways, whose function is typically modulated by interacting proteins acting as cofactors. However, how AGO1 is regulated and functions under Cu deficiency remains unknown. Here, we searched for AGO1 interactors in Arabidopsis thaliana plants expressing a functional AGO1 protein tagged with the Twin-Strep-tag (TST) polypeptide, grown under Cu-sufficient or Cu-deficient conditions. TST-AGO1 complexes were affinity purified, and proteins were identified using tandem MS. Interestingly, the iron superoxide dismutase 1 (FeSOD1) encoded by FSD1 was enriched in TST-AGO1 complexes purified from plants grown under Cu deficiency. Moreover, fsd1-2 mutant plants showed reduced levels of AGO1 compared with wild-type plants under Cu sufficiency, while both Cu-miRNA-specific and general AGO1 target mRNAs accumulated to higher levels in fsd1-2 plants under both Cu-deficient and Cu-sufficient conditions compared with wild-type plants. These findings suggest that FeSOD1 is essential for proper AGO1 function, and that its superoxide dismutase activity, which mitigates oxidative stress, enhances AGO1 stability, particularly under Cu deficiency.; AGO1 is essential for Cu-deficiency responses but is sensitive to oxidative stress. FeSOD1 interacts with AGO1 and protects it from superoxide radical-induced degradation, thereby preserving miRNA-mediated gene silencing pathways.This work was supported by grants or fellowships from MCIN/AEI/10.13039/501100011033 (co-financed by the European Regional Development Fund) [RTI2018-095118-A-I00, PID2021-122186OB-I00 and RYC-2017-21648 to AC; PID2021-127671NB-I00 to GR; PID2020-116940RB-I00 to LP and APG; BIO2017-83184-R and PID2020-114691RB-I00 to JAD; PRE2019-088439 to AEC], and from the Generalitat Valenciana (CIPROM/2022/21 to GR and JAD).Oxford University PressInstituto Universitario Mixto de Biología Molecular y Celular de PlantasGeneralitat ValencianaAgencia Estatal de InvestigaciónMinisterio de Ciencia e InnovaciónMinisterio de Ciencia, Innovación y UniversidadesRepositorio Institucional de la Universitat Politècnica de València Riunet20252025-07-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/226363reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 BIO2017-83184-R VIRUS DE PLANTAS: PATOGENOS Y TAMBIEN VECTORES PARA LA PRODUCCION DE PROTEINAS, METABOLITOS, RNAS Y NANOPARTICULASAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-114691RB-I00 BIOTECNOLOGIA DE VIRUS DE PLANTAS: VECTORES VIRALES Y ESTRATEGIAS DE RESISTENCIAAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-116940RB-I00 MECANISMOS DE ADAPTACION A CONDICIONES NO OPTIMAS DE COBRE Y HIERRO EN LEVADURAS Y PLANTASAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-095118-A-I00 COMPLEJOS ARGONAUTA1 DE PLANTAS: IDENTIFICACION DE SUS COMPONENTES PROTEICOS Y DE RNA, Y AJUSTE FINO DEL SILENCIAMIENTO MEDIANTE SU PROGRAMACION POR PEQUEÑOS RNAS ARTIFICIALESAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2021-122186OB-I00 PEQUEÑOS RNAS ARTIFICIALES: RNAI DE ULTIMA GENERACION PARA LA MEJORA DE CULTIVOS (ART-SRNAI)Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2021-127671NB-I00 INGENIERIA DE CIRCUITOS GENETICOS CON CABLEADO LUMINICO Y QUIMICOGeneralitat Valenciana https://doi.org/10.13039/501100003359 CIPROM%2F2022%2F21Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 RYC-2017-21648Ministerio de Ciencia e Innovación http://dx.doi.org/10.13039/501100004837 PRE2019-088439open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2263632026-06-13T07:49:27Z |
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15,811543 |