Virulence genotype and phenotype of two clinical isolates of Arcobacter butzleri obtained from patients with different pathologies

The surge in human arcobacteriosis has increased interest in determining the mechanisms involved in the pathogenesis of Arcobacter butzleri. Here, genomic analyses and in vitro Caco-2 infection, motility, urease and antimicrobial susceptibility testing (AST) assays were used to characterise the viru...

Descripción completa

Detalles Bibliográficos
Autores: Baztarrika Uria, Itsaso, Salazar Sánchez, Adrián, Hernaez Crespo, Silvia, López Mirones, José Israel, Canut Blasco, Andrés, Alonso Monsalve, Rodrigo, Martínez Ballesteros, Ilargi, Martínez Malax-Echevarría, Irati
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/67336
Acceso en línea:http://hdl.handle.net/10810/67336
Access Level:acceso abierto
Palabra clave:Arcobacter butzleri
caco-2 infection
comparative genomics
antimicrobial resistance
virulence
id ES_bb620622fcdaa493dc3dbb6009bf01f0
oai_identifier_str oai:addi.ehu.eus:10810/67336
network_acronym_str ES
network_name_str España
repository_id_str
spelling Virulence genotype and phenotype of two clinical isolates of Arcobacter butzleri obtained from patients with different pathologiesBaztarrika Uria, ItsasoSalazar Sánchez, AdriánHernaez Crespo, SilviaLópez Mirones, José IsraelCanut Blasco, AndrésAlonso Monsalve, RodrigoMartínez Ballesteros, IlargiMartínez Malax-Echevarría, IratiArcobacter butzlericaco-2 infectioncomparative genomicsantimicrobial resistancevirulenceThe surge in human arcobacteriosis has increased interest in determining the mechanisms involved in the pathogenesis of Arcobacter butzleri. Here, genomic analyses and in vitro Caco-2 infection, motility, urease and antimicrobial susceptibility testing (AST) assays were used to characterise the virulence and antimicrobial resistance (AMR) determinants of strains HC-1, isolated from a patient with travellers’ diarrhoea, and HC-2, isolated from another with pruritus. AMR determinants conferring resistance to tetracycline (tetO, present in both genomes) and to ampicillin and amoxicillin–clavulanic acid (bla3, present in HC-2) were identified. The same determinants associated with flagellum, chemotaxis, adhesion and invasion were detected in both, but HC-1 lacked eight flagellar genes. The urease cluster was only present in HC-1. Motility and urease tests confirmed the genetic differences between strains, but no genetic marker related to the inability of HC-2 to adhere and invade was identified. This inability could be conditioning the patient’s pathology.This research was funded by the Eusko Jaurlaritza [Grant number PA20/03]; and the University of the Basque Country UPV/EHU [Grant number GIU21/021]. I.B and A.S.-S are recipients of Ph.D. fellowships from the Eusko Jaurlaritza and the University of the Basque Country UPV/EHU, respectively.Springer Nature202420242023info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/67336reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://link.springer.com/article/10.1007/s00203-023-03709-3info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/© The Author(s) 2023. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.Atribución 3.0 Españaoai:addi.ehu.eus:10810/673362026-06-18T09:23:17Z
dc.title.none.fl_str_mv Virulence genotype and phenotype of two clinical isolates of Arcobacter butzleri obtained from patients with different pathologies
title Virulence genotype and phenotype of two clinical isolates of Arcobacter butzleri obtained from patients with different pathologies
spellingShingle Virulence genotype and phenotype of two clinical isolates of Arcobacter butzleri obtained from patients with different pathologies
Baztarrika Uria, Itsaso
Arcobacter butzleri
caco-2 infection
comparative genomics
antimicrobial resistance
virulence
title_short Virulence genotype and phenotype of two clinical isolates of Arcobacter butzleri obtained from patients with different pathologies
title_full Virulence genotype and phenotype of two clinical isolates of Arcobacter butzleri obtained from patients with different pathologies
title_fullStr Virulence genotype and phenotype of two clinical isolates of Arcobacter butzleri obtained from patients with different pathologies
title_full_unstemmed Virulence genotype and phenotype of two clinical isolates of Arcobacter butzleri obtained from patients with different pathologies
title_sort Virulence genotype and phenotype of two clinical isolates of Arcobacter butzleri obtained from patients with different pathologies
dc.creator.none.fl_str_mv Baztarrika Uria, Itsaso
Salazar Sánchez, Adrián
Hernaez Crespo, Silvia
López Mirones, José Israel
Canut Blasco, Andrés
Alonso Monsalve, Rodrigo
Martínez Ballesteros, Ilargi
Martínez Malax-Echevarría, Irati
author Baztarrika Uria, Itsaso
author_facet Baztarrika Uria, Itsaso
Salazar Sánchez, Adrián
Hernaez Crespo, Silvia
López Mirones, José Israel
Canut Blasco, Andrés
Alonso Monsalve, Rodrigo
Martínez Ballesteros, Ilargi
Martínez Malax-Echevarría, Irati
author_role author
author2 Salazar Sánchez, Adrián
Hernaez Crespo, Silvia
López Mirones, José Israel
Canut Blasco, Andrés
Alonso Monsalve, Rodrigo
Martínez Ballesteros, Ilargi
Martínez Malax-Echevarría, Irati
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Arcobacter butzleri
caco-2 infection
comparative genomics
antimicrobial resistance
virulence
topic Arcobacter butzleri
caco-2 infection
comparative genomics
antimicrobial resistance
virulence
description The surge in human arcobacteriosis has increased interest in determining the mechanisms involved in the pathogenesis of Arcobacter butzleri. Here, genomic analyses and in vitro Caco-2 infection, motility, urease and antimicrobial susceptibility testing (AST) assays were used to characterise the virulence and antimicrobial resistance (AMR) determinants of strains HC-1, isolated from a patient with travellers’ diarrhoea, and HC-2, isolated from another with pruritus. AMR determinants conferring resistance to tetracycline (tetO, present in both genomes) and to ampicillin and amoxicillin–clavulanic acid (bla3, present in HC-2) were identified. The same determinants associated with flagellum, chemotaxis, adhesion and invasion were detected in both, but HC-1 lacked eight flagellar genes. The urease cluster was only present in HC-1. Motility and urease tests confirmed the genetic differences between strains, but no genetic marker related to the inability of HC-2 to adhere and invade was identified. This inability could be conditioning the patient’s pathology.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/67336
url http://hdl.handle.net/10810/67336
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://link.springer.com/article/10.1007/s00203-023-03709-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418020781162496
score 15.301603

Similares en LA Referencia