Study of the Photobleaching Phenomenon to optimize Acquisition of 3D and 4D fluorescence images. A special scenario for trilinear and quadrilinear models.

Gómez Sánchez, Adrián; Alburquerque, Iker; Loza-Álvarez, Pablo; Ruckebusch, Cyril; Juan Capdevila, Anna de

Study of the Photobleaching Phenomenon to optimize Acquisition of 3D and 4D fluorescence images. A special scenario for trilinear and quadrilinear models.

Emission (3D) and excitation-emission (4D) fluorescence images allow covering wide excitation and emission spectral ranges and, hence, provide very complete information for a good characterization and location of fluorophores in samples. However, when the acquisition time of the image is too long, d...

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Detalles Bibliográficos
Autores:	Gómez Sánchez, Adrián, Alburquerque, Iker, Loza-Álvarez, Pablo, Ruckebusch, Cyril, Juan Capdevila, Anna de
Tipo de recurso:	artículo
Estado:	Versión publicada
Fecha de publicación:	2023
País:	España
Institución:	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:	Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:	oai:recercat.cat:2445/219784
Acceso en línea:	https://hdl.handle.net/2445/219784
Access Level:	acceso abierto
Palabra clave:	Fluorescència Imatges hiperespectrals Quimiometria Fluorescence Hyperspectral imaging Chemometrics

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spelling	Study of the Photobleaching Phenomenon to optimize Acquisition of 3D and 4D fluorescence images. A special scenario for trilinear and quadrilinear models.Gómez Sánchez, AdriánAlburquerque, IkerLoza-Álvarez, PabloRuckebusch, CyrilJuan Capdevila, Anna deFluorescènciaImatges hiperespectralsQuimiometriaFluorescenceHyperspectral imagingChemometricsEmission (3D) and excitation-emission (4D) fluorescence images allow covering wide excitation and emission spectral ranges and, hence, provide very complete information for a good characterization and location of fluorophores in samples. However, when the acquisition time of the image is too long, degradation of the fluorescence signal of compounds and sample photodamage can occur due to photobleaching. This phenomenon is due to the long exposure time of the sample to the light source and can hinder the detection and the proper characterization of the fluorophores in samples. The main purpose of this research is providing a methodology to obtain and interpret the information of fluorescence images for the characterization of samples without suffering the consequences of photobleaching. Such a goal implies a first thorough knowledge of the photobleaching phenomenon to adapt the fluorescence imaging measurement for an optimal characterization of the fluorophores present in samples. The proposed approach relies first on a study of time-series of 3D or 4D fluorescence images to characterize spatially and spectroscopically the fluorophores present in the samples and their photobleaching behaviour. Since photobleaching is fluorophore-dependent, the unmixing algorithm Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) is applied to the set of fluorescence images acquired as a function of time to understand the specific behaviour of every fluorophore. The characteristics of the photobleaching phenomenon and the nature of the fluorescence measurement offer a challenging scenario to look for adapted implementations of trilinear and quadrilinear models within the MCR framework. From the results obtained, appropriate instrumental settings are adopted for an image acquisition that allows the correct spatial and spectroscopic characterization of fluorophores in samples. To test the potential of this methodology, the characterization of thin cross-sections of the Oryza sativa (commonly called rice) root have been studied due to the co-occurrence of several natural fluorophores in vegetal tissues.Elsevier B.V.2025202520232025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion10 p.application/pdfhttps://hdl.handle.net/2445/219784Articles publicats en revistes (Enginyeria Química i Química Analítica)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1016/j.microc.2023.108899Microchemical Journal, 2023, vol. 191, p. 108899https://doi.org/10.1016/j.microc.2023.108899cc-by-nc-nd (c) Gómez Sánchez, Adrián, et al., 2023http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2197842026-05-29T05:05:01Z
dc.title.none.fl_str_mv	Study of the Photobleaching Phenomenon to optimize Acquisition of 3D and 4D fluorescence images. A special scenario for trilinear and quadrilinear models.
title	Study of the Photobleaching Phenomenon to optimize Acquisition of 3D and 4D fluorescence images. A special scenario for trilinear and quadrilinear models.
spellingShingle	Study of the Photobleaching Phenomenon to optimize Acquisition of 3D and 4D fluorescence images. A special scenario for trilinear and quadrilinear models. Gómez Sánchez, Adrián Fluorescència Imatges hiperespectrals Quimiometria Fluorescence Hyperspectral imaging Chemometrics
title_short	Study of the Photobleaching Phenomenon to optimize Acquisition of 3D and 4D fluorescence images. A special scenario for trilinear and quadrilinear models.
title_full	Study of the Photobleaching Phenomenon to optimize Acquisition of 3D and 4D fluorescence images. A special scenario for trilinear and quadrilinear models.
title_fullStr	Study of the Photobleaching Phenomenon to optimize Acquisition of 3D and 4D fluorescence images. A special scenario for trilinear and quadrilinear models.
title_full_unstemmed	Study of the Photobleaching Phenomenon to optimize Acquisition of 3D and 4D fluorescence images. A special scenario for trilinear and quadrilinear models.
title_sort	Study of the Photobleaching Phenomenon to optimize Acquisition of 3D and 4D fluorescence images. A special scenario for trilinear and quadrilinear models.
dc.creator.none.fl_str_mv	Gómez Sánchez, Adrián Alburquerque, Iker Loza-Álvarez, Pablo Ruckebusch, Cyril Juan Capdevila, Anna de
author	Gómez Sánchez, Adrián
author_facet	Gómez Sánchez, Adrián Alburquerque, Iker Loza-Álvarez, Pablo Ruckebusch, Cyril Juan Capdevila, Anna de
author_role	author
author2	Alburquerque, Iker Loza-Álvarez, Pablo Ruckebusch, Cyril Juan Capdevila, Anna de
author2_role	author author author author
dc.subject.none.fl_str_mv	Fluorescència Imatges hiperespectrals Quimiometria Fluorescence Hyperspectral imaging Chemometrics
topic	Fluorescència Imatges hiperespectrals Quimiometria Fluorescence Hyperspectral imaging Chemometrics
description	Emission (3D) and excitation-emission (4D) fluorescence images allow covering wide excitation and emission spectral ranges and, hence, provide very complete information for a good characterization and location of fluorophores in samples. However, when the acquisition time of the image is too long, degradation of the fluorescence signal of compounds and sample photodamage can occur due to photobleaching. This phenomenon is due to the long exposure time of the sample to the light source and can hinder the detection and the proper characterization of the fluorophores in samples. The main purpose of this research is providing a methodology to obtain and interpret the information of fluorescence images for the characterization of samples without suffering the consequences of photobleaching. Such a goal implies a first thorough knowledge of the photobleaching phenomenon to adapt the fluorescence imaging measurement for an optimal characterization of the fluorophores present in samples. The proposed approach relies first on a study of time-series of 3D or 4D fluorescence images to characterize spatially and spectroscopically the fluorophores present in the samples and their photobleaching behaviour. Since photobleaching is fluorophore-dependent, the unmixing algorithm Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) is applied to the set of fluorescence images acquired as a function of time to understand the specific behaviour of every fluorophore. The characteristics of the photobleaching phenomenon and the nature of the fluorescence measurement offer a challenging scenario to look for adapted implementations of trilinear and quadrilinear models within the MCR framework. From the results obtained, appropriate instrumental settings are adopted for an image acquisition that allows the correct spatial and spectroscopic characterization of fluorophores in samples. To test the potential of this methodology, the characterization of thin cross-sections of the Oryza sativa (commonly called rice) root have been studied due to the co-occurrence of several natural fluorophores in vegetal tissues.
publishDate	2023
dc.date.none.fl_str_mv	2023 2025 2025 2025
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	https://hdl.handle.net/2445/219784
url	https://hdl.handle.net/2445/219784
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	Reproducció del document publicat a: https://doi.org/10.1016/j.microc.2023.108899 Microchemical Journal, 2023, vol. 191, p. 108899 https://doi.org/10.1016/j.microc.2023.108899
dc.rights.none.fl_str_mv	cc-by-nc-nd (c) Gómez Sánchez, Adrián, et al., 2023 http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess
rights_invalid_str_mv	cc-by-nc-nd (c) Gómez Sánchez, Adrián, et al., 2023 http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	10 p. application/pdf
dc.publisher.none.fl_str_mv	Elsevier B.V.
publisher.none.fl_str_mv	Elsevier B.V.
dc.source.none.fl_str_mv	Articles publicats en revistes (Enginyeria Química i Química Analítica) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str	Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str	Recercat. Dipósit de la Recerca de Catalunya
collection	Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,811543

Study of the Photobleaching Phenomenon to optimize Acquisition of 3D and 4D fluorescence images. A special scenario for trilinear and quadrilinear models.

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