Effect of Photothermal Therapy Using Gold Nanoparticles Conjugated with Hyaluronic Acid in an Intracranial Murine Glioblastoma Model

Purpose: Glioblastoma multiforme (GBM) is the most common and aggressive malignant brain tumor. Conventional treatments for GBM include surgery, chemotherapy, radiotherapy, or a combination of these. However, emerging therapies, such as hyperthermia treatments, are being developed. One of these new...

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Autores: Domingo-Díez, J., Foti, A., Casanova-Carvajal, O., Marrodán, L., Granado, Noelia, Satriano, C., Martínez-Murillo, Ricardo, Serrano-Olmedo, J. J., Ramos-Gómez, Milagros
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/403830
Acceso en línea:http://hdl.handle.net/10261/403830
Access Level:acceso abierto
Palabra clave:Gold nanorods
glioblastoma
murine tumor model
photothermal therapy.
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spelling Effect of Photothermal Therapy Using Gold Nanoparticles Conjugated with Hyaluronic Acid in an Intracranial Murine Glioblastoma ModelDomingo-Díez, J.Foti, A.Casanova-Carvajal, O.Marrodán, L.Granado, NoeliaSatriano, C.Martínez-Murillo, RicardoSerrano-Olmedo, J. J.Ramos-Gómez, MilagrosGold nanorodsglioblastomamurine tumor modelphotothermal therapy.Purpose: Glioblastoma multiforme (GBM) is the most common and aggressive malignant brain tumor. Conventional treatments for GBM include surgery, chemotherapy, radiotherapy, or a combination of these. However, emerging therapies, such as hyperthermia treatments, are being developed. One of these new therapies is nanoparticle-mediated photothermal therapy (PTT), a non-invasive treatment that converts light into heat using photoagents such as plasmonic nanoparticles. High molecular weight hyaluronic acid (HA) has been described as a potential inhibitor of tumor progression and exhibits a high affinity for the CD44 receptor, which is present in GBM cells. The in vivo efficacy of gold nanorods (GNRs) biofunctionalized with HA-700kDa in PTT has been evaluated in a murine GBM model. Animals and Methods: Adult male C57/BL-6 mice (N=15), 3–8-month-old, were used for PTT experiments. CT2A cells were injected into the mouse brain to establish a GBM model. Tumor-bearing mice were randomly divided into three groups: Control (untreated, n=5), GNRs (injected with GNRs, n=5) and PTT-treated (injected with GNRs and treated with laser, n=5). After GNR injection, mice were irradiated with a laser at 0.98 A (250mW) for 25 min over three consecutive days. Results: As observed in the analysis of tumor sizes from all MR images, animals treated with a laser following GNR injection exhibited significantly smaller tumor sizes compared to control and GNR-treated animals one week after the treatment. In addition, PTT treatment led to a notable improvement in the exploratory behavior of the treated animals and an increase in their life expectancy compared to untreated control mice. Conclusion: This study demonstrates the efficacy of GNR-based-PTT, applied to an orthotopic tumor model, using GNRs biofunctionalized with HA to target GBM CT2A cells. The treatment resulted in a reduction in tumor mass and an extension of life expectancy in GNR-PTT treated mice.The authors would like to thank Soledad Martinez for the excellent technical assistance. This study was partially funded by the Ministerio de Ciencia, Innovación y Universidades of Spain, Refs.: PGC2018-097531-B-I00, PID2022-138881OB-I00 and PDC2022-133028-I00, funded by the European Union-Next Generation EU. CS acknowledges the financial supported by MUR in the framework of PRIN2022-PNRR call under project CoMu4CaT. The abstract of this paper was presented at the 7th Spanish Conference on Biomedical Applications of Nanomaterials (SBAN).Dove PressMinisterio de Ciencia, Innovación y Universidades (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2025202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/403830reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-097531-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-138881OB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PDC2022-133028-I00http://dx.doi.org/10.2147/IJN.S525462Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4038302026-05-22T06:33:51Z
dc.title.none.fl_str_mv Effect of Photothermal Therapy Using Gold Nanoparticles Conjugated with Hyaluronic Acid in an Intracranial Murine Glioblastoma Model
title Effect of Photothermal Therapy Using Gold Nanoparticles Conjugated with Hyaluronic Acid in an Intracranial Murine Glioblastoma Model
spellingShingle Effect of Photothermal Therapy Using Gold Nanoparticles Conjugated with Hyaluronic Acid in an Intracranial Murine Glioblastoma Model
Domingo-Díez, J.
Gold nanorods
glioblastoma
murine tumor model
photothermal therapy.
title_short Effect of Photothermal Therapy Using Gold Nanoparticles Conjugated with Hyaluronic Acid in an Intracranial Murine Glioblastoma Model
title_full Effect of Photothermal Therapy Using Gold Nanoparticles Conjugated with Hyaluronic Acid in an Intracranial Murine Glioblastoma Model
title_fullStr Effect of Photothermal Therapy Using Gold Nanoparticles Conjugated with Hyaluronic Acid in an Intracranial Murine Glioblastoma Model
title_full_unstemmed Effect of Photothermal Therapy Using Gold Nanoparticles Conjugated with Hyaluronic Acid in an Intracranial Murine Glioblastoma Model
title_sort Effect of Photothermal Therapy Using Gold Nanoparticles Conjugated with Hyaluronic Acid in an Intracranial Murine Glioblastoma Model
dc.creator.none.fl_str_mv Domingo-Díez, J.
Foti, A.
Casanova-Carvajal, O.
Marrodán, L.
Granado, Noelia
Satriano, C.
Martínez-Murillo, Ricardo
Serrano-Olmedo, J. J.
Ramos-Gómez, Milagros
author Domingo-Díez, J.
author_facet Domingo-Díez, J.
Foti, A.
Casanova-Carvajal, O.
Marrodán, L.
Granado, Noelia
Satriano, C.
Martínez-Murillo, Ricardo
Serrano-Olmedo, J. J.
Ramos-Gómez, Milagros
author_role author
author2 Foti, A.
Casanova-Carvajal, O.
Marrodán, L.
Granado, Noelia
Satriano, C.
Martínez-Murillo, Ricardo
Serrano-Olmedo, J. J.
Ramos-Gómez, Milagros
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Gold nanorods
glioblastoma
murine tumor model
photothermal therapy.
topic Gold nanorods
glioblastoma
murine tumor model
photothermal therapy.
description Purpose: Glioblastoma multiforme (GBM) is the most common and aggressive malignant brain tumor. Conventional treatments for GBM include surgery, chemotherapy, radiotherapy, or a combination of these. However, emerging therapies, such as hyperthermia treatments, are being developed. One of these new therapies is nanoparticle-mediated photothermal therapy (PTT), a non-invasive treatment that converts light into heat using photoagents such as plasmonic nanoparticles. High molecular weight hyaluronic acid (HA) has been described as a potential inhibitor of tumor progression and exhibits a high affinity for the CD44 receptor, which is present in GBM cells. The in vivo efficacy of gold nanorods (GNRs) biofunctionalized with HA-700kDa in PTT has been evaluated in a murine GBM model. Animals and Methods: Adult male C57/BL-6 mice (N=15), 3–8-month-old, were used for PTT experiments. CT2A cells were injected into the mouse brain to establish a GBM model. Tumor-bearing mice were randomly divided into three groups: Control (untreated, n=5), GNRs (injected with GNRs, n=5) and PTT-treated (injected with GNRs and treated with laser, n=5). After GNR injection, mice were irradiated with a laser at 0.98 A (250mW) for 25 min over three consecutive days. Results: As observed in the analysis of tumor sizes from all MR images, animals treated with a laser following GNR injection exhibited significantly smaller tumor sizes compared to control and GNR-treated animals one week after the treatment. In addition, PTT treatment led to a notable improvement in the exploratory behavior of the treated animals and an increase in their life expectancy compared to untreated control mice. Conclusion: This study demonstrates the efficacy of GNR-based-PTT, applied to an orthotopic tumor model, using GNRs biofunctionalized with HA to target GBM CT2A cells. The treatment resulted in a reduction in tumor mass and an extension of life expectancy in GNR-PTT treated mice.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/403830
url http://hdl.handle.net/10261/403830
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-138881OB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PDC2022-133028-I00
http://dx.doi.org/10.2147/IJN.S525462
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Dove Press
publisher.none.fl_str_mv Dove Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
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