Expression Profiling in Pinus pinaster in Response to Infection with the Pine Wood Nematode Bursaphelenchus xylophilus

Forests are essential resources on a global scale, not only for the ecological benefits, but also for economical and landscape purposes. However, in recent years, a large number of forest species have suffered a serious decline, with maritime pine being one of the most affected. In Portugal, the mar...

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Authors: Gaspar, Daniel, Trindade, Candida, Usié Chimenos, Anabel, Meireles, Brígida, Barbosa, Pedro, Fortes, Ana, Pesquita, Cátia, Costa, Rita, Ramos, António Marcos
Format: article
Status:Published version
Publication Date:2017
Country:España
Institution:Universidad Pontificia Comillas ICAI-ICADE
Repository:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/469091
Online Access:https://doi.org/10.3390/f8080279
https://hdl.handle.net/10459.1/469091
Access Level:Open access
Keyword:RNA-sequencing
Transcriptome analysis
Gene expression
Bursaphelenchus xylophilus
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spelling Expression Profiling in Pinus pinaster in Response to Infection with the Pine Wood Nematode Bursaphelenchus xylophilusGaspar, DanielTrindade, CandidaUsié Chimenos, AnabelMeireles, BrígidaBarbosa, PedroFortes, AnaPesquita, CátiaCosta, RitaRamos, António MarcosRNA-sequencingTranscriptome analysisGene expressionBursaphelenchus xylophilusForests are essential resources on a global scale, not only for the ecological benefits, but also for economical and landscape purposes. However, in recent years, a large number of forest species have suffered a serious decline, with maritime pine being one of the most affected. In Portugal, the maritime pine forest has been devastated by the pine wood nematode (PWN), the causal agent of pine wilt disease. In this study, RNA-Seq data was used to characterize the maritime pine response to infection with PWN, by determining the differentially expressed genes and identifying the regulatory networks and pathways associated. The analyses showed clear differences between an early response that occurs immediately after inoculation and a late response that is observed seven days after inoculation. Moreover, differentially expressed genes related to secondary metabolism, oxidative stress and defense against pathogen infection were identified over different time points. These results provide new insights about the molecular mechanisms and metabolic pathways involved in the response of Pinus pinaster against PWN infection, which will be a useful resource in follow-up studies and for future breeding programs to select plants with lower susceptibility to this disease.MDPI2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.3390/f8080279https://hdl.handle.net/10459.1/469091reponame:Repositori Obert UdL instname:Universidad Pontificia Comillas ICAI-ICADEInglésReproducció del document publicat a https://doi.org/10.3390/f8080279Forests, 2017, vol. 8, núm. 8, 279cc-by, (c) Daniel Gaspar et al., 2017Attribution 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/4690912026-06-24T12:42:17Z
dc.title.none.fl_str_mv Expression Profiling in Pinus pinaster in Response to Infection with the Pine Wood Nematode Bursaphelenchus xylophilus
title Expression Profiling in Pinus pinaster in Response to Infection with the Pine Wood Nematode Bursaphelenchus xylophilus
spellingShingle Expression Profiling in Pinus pinaster in Response to Infection with the Pine Wood Nematode Bursaphelenchus xylophilus
Gaspar, Daniel
RNA-sequencing
Transcriptome analysis
Gene expression
Bursaphelenchus xylophilus
title_short Expression Profiling in Pinus pinaster in Response to Infection with the Pine Wood Nematode Bursaphelenchus xylophilus
title_full Expression Profiling in Pinus pinaster in Response to Infection with the Pine Wood Nematode Bursaphelenchus xylophilus
title_fullStr Expression Profiling in Pinus pinaster in Response to Infection with the Pine Wood Nematode Bursaphelenchus xylophilus
title_full_unstemmed Expression Profiling in Pinus pinaster in Response to Infection with the Pine Wood Nematode Bursaphelenchus xylophilus
title_sort Expression Profiling in Pinus pinaster in Response to Infection with the Pine Wood Nematode Bursaphelenchus xylophilus
dc.creator.none.fl_str_mv Gaspar, Daniel
Trindade, Candida
Usié Chimenos, Anabel
Meireles, Brígida
Barbosa, Pedro
Fortes, Ana
Pesquita, Cátia
Costa, Rita
Ramos, António Marcos
author Gaspar, Daniel
author_facet Gaspar, Daniel
Trindade, Candida
Usié Chimenos, Anabel
Meireles, Brígida
Barbosa, Pedro
Fortes, Ana
Pesquita, Cátia
Costa, Rita
Ramos, António Marcos
author_role author
author2 Trindade, Candida
Usié Chimenos, Anabel
Meireles, Brígida
Barbosa, Pedro
Fortes, Ana
Pesquita, Cátia
Costa, Rita
Ramos, António Marcos
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv RNA-sequencing
Transcriptome analysis
Gene expression
Bursaphelenchus xylophilus
topic RNA-sequencing
Transcriptome analysis
Gene expression
Bursaphelenchus xylophilus
description Forests are essential resources on a global scale, not only for the ecological benefits, but also for economical and landscape purposes. However, in recent years, a large number of forest species have suffered a serious decline, with maritime pine being one of the most affected. In Portugal, the maritime pine forest has been devastated by the pine wood nematode (PWN), the causal agent of pine wilt disease. In this study, RNA-Seq data was used to characterize the maritime pine response to infection with PWN, by determining the differentially expressed genes and identifying the regulatory networks and pathways associated. The analyses showed clear differences between an early response that occurs immediately after inoculation and a late response that is observed seven days after inoculation. Moreover, differentially expressed genes related to secondary metabolism, oxidative stress and defense against pathogen infection were identified over different time points. These results provide new insights about the molecular mechanisms and metabolic pathways involved in the response of Pinus pinaster against PWN infection, which will be a useful resource in follow-up studies and for future breeding programs to select plants with lower susceptibility to this disease.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3390/f8080279
https://hdl.handle.net/10459.1/469091
url https://doi.org/10.3390/f8080279
https://hdl.handle.net/10459.1/469091
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a https://doi.org/10.3390/f8080279
Forests, 2017, vol. 8, núm. 8, 279
dc.rights.none.fl_str_mv cc-by, (c) Daniel Gaspar et al., 2017
Attribution 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by, (c) Daniel Gaspar et al., 2017
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universidad Pontificia Comillas ICAI-ICADE
instname_str Universidad Pontificia Comillas ICAI-ICADE
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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