Synthesis, Experimental and Computational Evaluation of SERAAK1 as a 5-HT2A Receptor Ligand

Many drug discovery efforts have identified potentially promising molecules; however, a common limitation of these reports is the lack of further experimental confirmation of pharmacokinetic properties and behavioral effects of discovered compounds. In this study, we aim to address this limitation....

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Detalles Bibliográficos
Autores: Zięba, Agata, Kędzierska, Ewa, Jastrzębski, Michał K., Karcz, Tadeusz, Olejarz-Maciej, Agnieszka, Sumara, Agata, Laitinen, Tuomo, Wróbel, Tomasz M., Fornal, Emilia, Castro Pérez, María de los Ángeles, Kaczor, Agnieszka A.
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:minerva.usc.gal:10347/41629
Acceso en línea:https://hdl.handle.net/10347/41629
Access Level:acceso abierto
Palabra clave:5-HT2A receptor
ADMET
Antidepressants
Antipsychotics
In vivo studies
Molecular modeling
320808 Mecanismos de acción de los medicamentos
320909 Psicofarmacología
320990 Farmacología experimental
Descripción
Sumario:Many drug discovery efforts have identified potentially promising molecules; however, a common limitation of these reports is the lack of further experimental confirmation of pharmacokinetic properties and behavioral effects of discovered compounds. In this study, we aim to address this limitation. Therefore, we build on our previous virtual screening campaign by synthesizing, analyzing in silico, and evaluating experimentally the SERAAK1 compound, which was initially identified as a ligand for 5-HT1A, 5-HT2A, and D2 receptors. Through these investigations, we discovered that SERAAK1 binds to the orthosteric pocket of the 5-HT2A receptor in a similar mechanism to that known for marketed antipsychotic medications. Molecular dynamics simulations revealed that the SERAAK1 compound remains stable in the orthosteric binding pocket of the 5-HT2A receptor. The determination of the ADMET parameters indicated the directions for further optimization of the compounds. In vivo studies demonstrated the anxiolytic and antidepressant properties of the SERAAK1 compound.