New evidence of cryptic speciation in the family Longidoridae (Nematoda: Dorylaimida)

Longidorid nematodes comprise more than 500 species, and Longidorus and Xiphinema are the most diversified, prevalent, and cosmopolitan genera within plant‐parasitic nematodes. This increases the risk of species misidentification. We conducted an integrative morphometric and genetic study on two lon...

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Detalles Bibliográficos
Autores: Cai, Ruihang, Archidona-Yuste, Antonio, Cantalapiedra-Navarrete, C., Palomares Rius, Juan E., Castillo, Pablo
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/227973
Acceso en línea:http://hdl.handle.net/10261/227973
Access Level:acceso abierto
Palabra clave:Bayesian inference
Cytochrome oxidase c subunit 1
Expansion domains of the large ribosomal subunit (28S)
Integrative taxonomy
Plant‐parasitic nematodes
Descripción
Sumario:Longidorid nematodes comprise more than 500 species, and Longidorus and Xiphinema are the most diversified, prevalent, and cosmopolitan genera within plant‐parasitic nematodes. This increases the risk of species misidentification. We conducted an integrative morphometric and genetic study on two longidorid species to elucidate the existence of new cases of cryptic speciation within the genera Longidorus and Xiphinema. Detailed morphological, morphometrical, multivariate, and genetic studies were carried out, and mitochondrial and nuclear haploweb analyses were used to differentiate species within the L. iliturgiensis and X. hispanum complexes. Species delimitation using multivariate and haplonet tools of L. iliturgiensis species complex clearly separated L. tabernensis sp. nov. from L. iliturgiensis and L. indalus, and X. subbaetense sp. nov. from X. hispanum and X. adenohystherum. D2‐D3, partial 18S, and partial coxI regions were used for inferring their phylogenetic relationships with other species in each genus. The present study provides new insights into the diversity of Longidorus and Xiphinema species detected in southern Spain, and new evidence of cryptic speciation in both genera. These results support our hypothesis that the biodiversity of Longidoridae in southern Europe is higher than previously supposed and is still not fully clarified.