Nasal microbial diversity is associated with survival in piglets infected by a highly virulent PRRSV-1 strain
Porcine reproductive and respiratory syndrome virus (PRRSV) is a major threat to swine industry worldwide, especially virulent variants arising during the last years, such as Spanish PRRSV-1 Rosalia strain. The role of the nasal microbiota in respiratory viral infections is still to be unveiled but...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:308617 |
| Acceso en línea: | https://ddd.uab.cat/record/308617 https://dx.doi.org/urn:doi:10.1186/s42523-024-00371-y |
| Access Level: | acceso abierto |
| Palabra clave: | Pig Swine Virus PRRSV Nasal microbiota |
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Nasal microbial diversity is associated with survival in piglets infected by a highly virulent PRRSV-1 strainObregon-Gutierrez, Pau|||0000-0002-6897-2792Cortey, Martí|||0000-0003-1126-3423Martin-Valls, Gerard|||0000-0003-3302-0692Clilverd, Hepzibar|||0000-0002-4309-7506Correa-Fiz, Florencia|||0000-0002-9459-5871Aragón, Virginia|||0000-0002-3470-6015Mateu de Antonio, Enrique María|||0000-0003-2715-6032PigSwineVirusPRRSVNasal microbiotaPorcine reproductive and respiratory syndrome virus (PRRSV) is a major threat to swine industry worldwide, especially virulent variants arising during the last years, such as Spanish PRRSV-1 Rosalia strain. The role of the nasal microbiota in respiratory viral infections is still to be unveiled but may be promisingly related with the health status of the animals and thus, their susceptibility. The goal of this project was to study the nasal microbiota composition of piglets during a highly virulent PRRSV-1 outbreak comparing animals that died due to the infection with animals that survived it. The microbiota composition was inferred by V3-V4 regions of the 16S rRNA gene sequencing and bioinformatics analysis. To deepen the analysis, we added samples taken from piglets before the outbreak as well as from the sows giving birth to piglets under study. Piglets that survived the PRRSV-1 outbreak reported a more diverse and different nasal microbiota at three weeks of age compared to piglets dying, which was highly related with the litter of origin and the sow of the piglets. In addition, a high abundance of classical swine nasal colonizers belonging to genera such as Bergeyella, Glaesserella, Neisseria and Moraxella (among others), was related with good outcome. On the other hand, a dysbiotic community dominated by Escherichia and a different clade of Moraxella was found in piglets with bad outcome. Moreover, samples taken before the outbreak showed similar dynamics prior to virulent PRRSV-1 arrival, suggesting that microbiota-related susceptibility was already occurring in the animals and that the increase in mortality seen was related to the new highly virulent strain. Our study suggests that the susceptibility to an infection such as PRRSV could be related to the nasal microbiota composition at the moment of infection and may serve as starting point to explore animal resilience. Since the dysbiosis detected as an initial response to infection may be not specific for this virus, further investigations should explore this phenomenon in the context of other viral infections. The online version contains supplementary material available at 10.1186/s42523-024-00371-y. 22025-01-0120252025-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/308617https://dx.doi.org/urn:doi:10.1186/s42523-024-00371-yreponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 FPU19/02126Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PCI2020-120688-2open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3086172026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Nasal microbial diversity is associated with survival in piglets infected by a highly virulent PRRSV-1 strain |
| title |
Nasal microbial diversity is associated with survival in piglets infected by a highly virulent PRRSV-1 strain |
| spellingShingle |
Nasal microbial diversity is associated with survival in piglets infected by a highly virulent PRRSV-1 strain Obregon-Gutierrez, Pau|||0000-0002-6897-2792 Pig Swine Virus PRRSV Nasal microbiota |
| title_short |
Nasal microbial diversity is associated with survival in piglets infected by a highly virulent PRRSV-1 strain |
| title_full |
Nasal microbial diversity is associated with survival in piglets infected by a highly virulent PRRSV-1 strain |
| title_fullStr |
Nasal microbial diversity is associated with survival in piglets infected by a highly virulent PRRSV-1 strain |
| title_full_unstemmed |
Nasal microbial diversity is associated with survival in piglets infected by a highly virulent PRRSV-1 strain |
| title_sort |
Nasal microbial diversity is associated with survival in piglets infected by a highly virulent PRRSV-1 strain |
| dc.creator.none.fl_str_mv |
Obregon-Gutierrez, Pau|||0000-0002-6897-2792 Cortey, Martí|||0000-0003-1126-3423 Martin-Valls, Gerard|||0000-0003-3302-0692 Clilverd, Hepzibar|||0000-0002-4309-7506 Correa-Fiz, Florencia|||0000-0002-9459-5871 Aragón, Virginia|||0000-0002-3470-6015 Mateu de Antonio, Enrique María|||0000-0003-2715-6032 |
| author |
Obregon-Gutierrez, Pau|||0000-0002-6897-2792 |
| author_facet |
Obregon-Gutierrez, Pau|||0000-0002-6897-2792 Cortey, Martí|||0000-0003-1126-3423 Martin-Valls, Gerard|||0000-0003-3302-0692 Clilverd, Hepzibar|||0000-0002-4309-7506 Correa-Fiz, Florencia|||0000-0002-9459-5871 Aragón, Virginia|||0000-0002-3470-6015 Mateu de Antonio, Enrique María|||0000-0003-2715-6032 |
| author_role |
author |
| author2 |
Cortey, Martí|||0000-0003-1126-3423 Martin-Valls, Gerard|||0000-0003-3302-0692 Clilverd, Hepzibar|||0000-0002-4309-7506 Correa-Fiz, Florencia|||0000-0002-9459-5871 Aragón, Virginia|||0000-0002-3470-6015 Mateu de Antonio, Enrique María|||0000-0003-2715-6032 |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
Pig Swine Virus PRRSV Nasal microbiota |
| topic |
Pig Swine Virus PRRSV Nasal microbiota |
| description |
Porcine reproductive and respiratory syndrome virus (PRRSV) is a major threat to swine industry worldwide, especially virulent variants arising during the last years, such as Spanish PRRSV-1 Rosalia strain. The role of the nasal microbiota in respiratory viral infections is still to be unveiled but may be promisingly related with the health status of the animals and thus, their susceptibility. The goal of this project was to study the nasal microbiota composition of piglets during a highly virulent PRRSV-1 outbreak comparing animals that died due to the infection with animals that survived it. The microbiota composition was inferred by V3-V4 regions of the 16S rRNA gene sequencing and bioinformatics analysis. To deepen the analysis, we added samples taken from piglets before the outbreak as well as from the sows giving birth to piglets under study. Piglets that survived the PRRSV-1 outbreak reported a more diverse and different nasal microbiota at three weeks of age compared to piglets dying, which was highly related with the litter of origin and the sow of the piglets. In addition, a high abundance of classical swine nasal colonizers belonging to genera such as Bergeyella, Glaesserella, Neisseria and Moraxella (among others), was related with good outcome. On the other hand, a dysbiotic community dominated by Escherichia and a different clade of Moraxella was found in piglets with bad outcome. Moreover, samples taken before the outbreak showed similar dynamics prior to virulent PRRSV-1 arrival, suggesting that microbiota-related susceptibility was already occurring in the animals and that the increase in mortality seen was related to the new highly virulent strain. Our study suggests that the susceptibility to an infection such as PRRSV could be related to the nasal microbiota composition at the moment of infection and may serve as starting point to explore animal resilience. Since the dysbiosis detected as an initial response to infection may be not specific for this virus, further investigations should explore this phenomenon in the context of other viral infections. The online version contains supplementary material available at 10.1186/s42523-024-00371-y. |
| publishDate |
2025 |
| dc.date.none.fl_str_mv |
2 2025-01-01 2025 2025-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/308617 https://dx.doi.org/urn:doi:10.1186/s42523-024-00371-y |
| url |
https://ddd.uab.cat/record/308617 https://dx.doi.org/urn:doi:10.1186/s42523-024-00371-y |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 FPU19/02126 Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PCI2020-120688-2 |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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application/pdf |
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