Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization

BACKGROUND: Lactococcus garvieae is a bacterial pathogen that affects different animal species in addition to humans. Despite the widespread distribution and emerging clinical significance of L. garvieae in both veterinary and human medicine, there is almost a complete lack of knowledge about the ge...

Descripción completa

Detalles Bibliográficos
Autores: Aguado-Urda, Mónica, Lopez-Campos, Guillermo, Fernández-Garayzábal, José F, Martin-Sanchez, Fernando, Gibello, Alicia, Domínguez, Lucas, Blanco, María del Mar
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/6815
Acceso en línea:http://hdl.handle.net/20.500.12105/6815
Access Level:acceso abierto
Palabra clave:Comparative Genomic Hybridization
Computational Biology
Computer Simulation
DNA, Bacterial
Databases, Genetic
Lactococcus
Oligonucleotide Array Sequence Analysis
Species Specificity
Streptococcus pneumoniae
Genome, Bacterial
id ES_baf54bc006ed1b85192d4e974898bd65
oai_identifier_str oai:repisalud.isciii.es:20.500.12105/6815
network_acronym_str ES
network_name_str España
repository_id_str
spelling Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridizationAguado-Urda, MónicaLopez-Campos, GuillermoFernández-Garayzábal, José FMartin-Sanchez, FernandoGibello, AliciaDomínguez, LucasBlanco, María del MarComparative Genomic HybridizationComputational BiologyComputer SimulationDNA, BacterialDatabases, GeneticLactococcusOligonucleotide Array Sequence AnalysisSpecies SpecificityStreptococcus pneumoniaeGenome, BacterialBACKGROUND: Lactococcus garvieae is a bacterial pathogen that affects different animal species in addition to humans. Despite the widespread distribution and emerging clinical significance of L. garvieae in both veterinary and human medicine, there is almost a complete lack of knowledge about the genetic content of this microorganism. In the present study, the genomic content of L. garvieae CECT 4531 was analysed using bioinformatics tools and microarray-based comparative genomic hybridization (CGH) experiments. Lactococcus lactis subsp. lactis IL1403 and Streptococcus pneumoniae TIGR4 were used as reference microorganisms. RESULTS: The combination and integration of in silico analyses and in vitro CGH experiments, performed in comparison with the reference microorganisms, allowed establishment of an inter-species hybridization framework with a detection threshold based on a sequence similarity of >or= 70%. With this threshold value, 267 genes were identified as having an analogue in L. garvieae, most of which (n = 258) have been documented for the first time in this pathogen. Most of the genes are related to ribosomal, sugar metabolism or energy conversion systems. Some of the identified genes, such as als and mycA, could be involved in the pathogenesis of L. garvieae infections. CONCLUSIONS: In this study, we identified 267 genes that were potentially present in L. garvieae CECT 4531. Some of the identified genes could be involved in the pathogenesis of L. garvieae infections. These results provide the first insight into the genome content of L. garvieae.BioMed Central (BMC)Ministerio de Ciencia e Innovación (España)20182018-12-1120102010-03-1620102010-03-16research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/6815reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/68152026-06-12T12:43:37Z
dc.title.none.fl_str_mv Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization
title Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization
spellingShingle Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization
Aguado-Urda, Mónica
Comparative Genomic Hybridization
Computational Biology
Computer Simulation
DNA, Bacterial
Databases, Genetic
Lactococcus
Oligonucleotide Array Sequence Analysis
Species Specificity
Streptococcus pneumoniae
Genome, Bacterial
title_short Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization
title_full Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization
title_fullStr Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization
title_full_unstemmed Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization
title_sort Analysis of the genome content of Lactococcus garvieae by genomic interspecies microarray hybridization
dc.creator.none.fl_str_mv Aguado-Urda, Mónica
Lopez-Campos, Guillermo
Fernández-Garayzábal, José F
Martin-Sanchez, Fernando
Gibello, Alicia
Domínguez, Lucas
Blanco, María del Mar
author Aguado-Urda, Mónica
author_facet Aguado-Urda, Mónica
Lopez-Campos, Guillermo
Fernández-Garayzábal, José F
Martin-Sanchez, Fernando
Gibello, Alicia
Domínguez, Lucas
Blanco, María del Mar
author_role author
author2 Lopez-Campos, Guillermo
Fernández-Garayzábal, José F
Martin-Sanchez, Fernando
Gibello, Alicia
Domínguez, Lucas
Blanco, María del Mar
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
dc.subject.none.fl_str_mv Comparative Genomic Hybridization
Computational Biology
Computer Simulation
DNA, Bacterial
Databases, Genetic
Lactococcus
Oligonucleotide Array Sequence Analysis
Species Specificity
Streptococcus pneumoniae
Genome, Bacterial
topic Comparative Genomic Hybridization
Computational Biology
Computer Simulation
DNA, Bacterial
Databases, Genetic
Lactococcus
Oligonucleotide Array Sequence Analysis
Species Specificity
Streptococcus pneumoniae
Genome, Bacterial
description BACKGROUND: Lactococcus garvieae is a bacterial pathogen that affects different animal species in addition to humans. Despite the widespread distribution and emerging clinical significance of L. garvieae in both veterinary and human medicine, there is almost a complete lack of knowledge about the genetic content of this microorganism. In the present study, the genomic content of L. garvieae CECT 4531 was analysed using bioinformatics tools and microarray-based comparative genomic hybridization (CGH) experiments. Lactococcus lactis subsp. lactis IL1403 and Streptococcus pneumoniae TIGR4 were used as reference microorganisms. RESULTS: The combination and integration of in silico analyses and in vitro CGH experiments, performed in comparison with the reference microorganisms, allowed establishment of an inter-species hybridization framework with a detection threshold based on a sequence similarity of >or= 70%. With this threshold value, 267 genes were identified as having an analogue in L. garvieae, most of which (n = 258) have been documented for the first time in this pathogen. Most of the genes are related to ribosomal, sugar metabolism or energy conversion systems. Some of the identified genes, such as als and mycA, could be involved in the pathogenesis of L. garvieae infections. CONCLUSIONS: In this study, we identified 267 genes that were potentially present in L. garvieae CECT 4531. Some of the identified genes could be involved in the pathogenesis of L. garvieae infections. These results provide the first insight into the genome content of L. garvieae.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-03-16
2010
2010-03-16
2018
2018-12-11
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/6815
url http://hdl.handle.net/20.500.12105/6815
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central (BMC)
publisher.none.fl_str_mv BioMed Central (BMC)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869417983088001024
score 15,811543

Similares en LA Referencia