Analysis of Self-Incompatibility and Genetic Diversity in Diploid and Hexaploid Plum Genotypes

During the last decade, S-genotyping has been extensively investigated in fruit tree crops such as those belonging to the Prunus genus, including plums. In plums, S-allele typing has been largely studied in diploid species but works are scarcer in polyploid species due to the complexity of the polyp...

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Autores: Abdallah, Donia, Baraket, Ghada, Pérez Méndez, Verónica, Mustapha, Sana Ben, Salhi-Hannachi, Amel, Hormaza Urroz, José Ignacio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/205368
Acceso en línea:http://hdl.handle.net/10261/205368
Access Level:acceso abierto
Palabra clave:Plums
Polyploidy
S-genotyping
Self-(in)compatibility
Pollination
Pollen tube
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spelling Analysis of Self-Incompatibility and Genetic Diversity in Diploid and Hexaploid Plum GenotypesAbdallah, DoniaBaraket, GhadaPérez Méndez, VerónicaMustapha, Sana BenSalhi-Hannachi, AmelHormaza Urroz, José IgnacioPlumsPolyploidyS-genotypingSelf-(in)compatibilityPollinationPollen tubeDuring the last decade, S-genotyping has been extensively investigated in fruit tree crops such as those belonging to the Prunus genus, including plums. In plums, S-allele typing has been largely studied in diploid species but works are scarcer in polyploid species due to the complexity of the polyploid genome. This study was conducted in order to analyze the S-genotypes of 30 diploid P. salicina, 17 of them reported here for the first time, and 29 hexaploid plums (24 of P. domestica and 5 of P. insititia). PCR analysis allowed identifying nine S-alleles in the P. salicina samples allocating the 30 accessions in 16 incompatibility groups, two of them identified here for the first time. In addition, pollen tube growth was studied in self-pollinated flowers of 17 Tunisian P. salicina under the microscope. In 16 samples, including one carrying the Se allele, which has been correlated with self-compatibility, the pollen tubes were arrested in the style. Only in one cultivar (“Bedri”), the pollen tubes reached the base of the style. Twelve S-alleles were identified in the 24 P. domestica and 5 P. insititia accessions, assigning accessions in 16 S-genotypes. S-genotyping results were combined with nine SSR loci to analyze genetic diversity. Results showed a close genetic relationship between P. domestica and P. salicina and between P. domestica and P. insititia corroborating that S-locus genotyping is suitable for molecular fingerprinting in diploid and polyploid Prunus species.This research was supported by the Tunisian “Ministère de l’Enseignement Supérieur et de la Recherche Scientifique” and “Ministerio de Economía y Competitividad – European Regional Development Fund, European Union (AGL2016-77267-R).”Peer reviewedFrontiers MediaMinistère de l’Enseignement Supérieur et de la Recherche Scientifique (Tunisie)Ministerio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/205368reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-77267-Rhttps://doi.org/10.3389/fpls.2019.00896Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2053682026-05-22T06:33:51Z
dc.title.none.fl_str_mv Analysis of Self-Incompatibility and Genetic Diversity in Diploid and Hexaploid Plum Genotypes
title Analysis of Self-Incompatibility and Genetic Diversity in Diploid and Hexaploid Plum Genotypes
spellingShingle Analysis of Self-Incompatibility and Genetic Diversity in Diploid and Hexaploid Plum Genotypes
Abdallah, Donia
Plums
Polyploidy
S-genotyping
Self-(in)compatibility
Pollination
Pollen tube
title_short Analysis of Self-Incompatibility and Genetic Diversity in Diploid and Hexaploid Plum Genotypes
title_full Analysis of Self-Incompatibility and Genetic Diversity in Diploid and Hexaploid Plum Genotypes
title_fullStr Analysis of Self-Incompatibility and Genetic Diversity in Diploid and Hexaploid Plum Genotypes
title_full_unstemmed Analysis of Self-Incompatibility and Genetic Diversity in Diploid and Hexaploid Plum Genotypes
title_sort Analysis of Self-Incompatibility and Genetic Diversity in Diploid and Hexaploid Plum Genotypes
dc.creator.none.fl_str_mv Abdallah, Donia
Baraket, Ghada
Pérez Méndez, Verónica
Mustapha, Sana Ben
Salhi-Hannachi, Amel
Hormaza Urroz, José Ignacio
author Abdallah, Donia
author_facet Abdallah, Donia
Baraket, Ghada
Pérez Méndez, Verónica
Mustapha, Sana Ben
Salhi-Hannachi, Amel
Hormaza Urroz, José Ignacio
author_role author
author2 Baraket, Ghada
Pérez Méndez, Verónica
Mustapha, Sana Ben
Salhi-Hannachi, Amel
Hormaza Urroz, José Ignacio
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministère de l’Enseignement Supérieur et de la Recherche Scientifique (Tunisie)
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Plums
Polyploidy
S-genotyping
Self-(in)compatibility
Pollination
Pollen tube
topic Plums
Polyploidy
S-genotyping
Self-(in)compatibility
Pollination
Pollen tube
description During the last decade, S-genotyping has been extensively investigated in fruit tree crops such as those belonging to the Prunus genus, including plums. In plums, S-allele typing has been largely studied in diploid species but works are scarcer in polyploid species due to the complexity of the polyploid genome. This study was conducted in order to analyze the S-genotypes of 30 diploid P. salicina, 17 of them reported here for the first time, and 29 hexaploid plums (24 of P. domestica and 5 of P. insititia). PCR analysis allowed identifying nine S-alleles in the P. salicina samples allocating the 30 accessions in 16 incompatibility groups, two of them identified here for the first time. In addition, pollen tube growth was studied in self-pollinated flowers of 17 Tunisian P. salicina under the microscope. In 16 samples, including one carrying the Se allele, which has been correlated with self-compatibility, the pollen tubes were arrested in the style. Only in one cultivar (“Bedri”), the pollen tubes reached the base of the style. Twelve S-alleles were identified in the 24 P. domestica and 5 P. insititia accessions, assigning accessions in 16 S-genotypes. S-genotyping results were combined with nine SSR loci to analyze genetic diversity. Results showed a close genetic relationship between P. domestica and P. salicina and between P. domestica and P. insititia corroborating that S-locus genotyping is suitable for molecular fingerprinting in diploid and polyploid Prunus species.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/205368
url http://hdl.handle.net/10261/205368
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-77267-R
https://doi.org/10.3389/fpls.2019.00896
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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