Effects of avian Plasmodium exposure on the microbiota of Culex pipiens

Malaria parasites (Plasmodium spp.) are mosquito-borne parasites that infect humans and wildlife. Several studies support the role of mosquito microbiota as a major driver of Plasmodium transmission, although studies on wildlife malaria are typically neglected. Here, we used a 16S rRNA metabarcoding...

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Authors: Garrigós, Marta, García Ruiz, Olaya, Enkvist, Charlotte, García-López, María José, Moreno-Indias, Isabel, Ruiz-López, María José, Veiga, Jesús, Figuerola, Jordi, Videvall, Elin, Martínez de la Puente, Josué
Format: article
Status:Published version
Publication Date:2025
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/405361
Online Access:http://hdl.handle.net/10261/405361
Access Level:Open access
Keyword:Avian malaria
Bacteria
Microbiome
Mosquitoes
Vector-borne pathogens
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dc.title.none.fl_str_mv Effects of avian Plasmodium exposure on the microbiota of Culex pipiens
title Effects of avian Plasmodium exposure on the microbiota of Culex pipiens
spellingShingle Effects of avian Plasmodium exposure on the microbiota of Culex pipiens
Garrigós, Marta
Avian malaria
Bacteria
Microbiome
Mosquitoes
Vector-borne pathogens
title_short Effects of avian Plasmodium exposure on the microbiota of Culex pipiens
title_full Effects of avian Plasmodium exposure on the microbiota of Culex pipiens
title_fullStr Effects of avian Plasmodium exposure on the microbiota of Culex pipiens
title_full_unstemmed Effects of avian Plasmodium exposure on the microbiota of Culex pipiens
title_sort Effects of avian Plasmodium exposure on the microbiota of Culex pipiens
dc.creator.none.fl_str_mv Garrigós, Marta
García Ruiz, Olaya
Enkvist, Charlotte
García-López, María José
Moreno-Indias, Isabel
Ruiz-López, María José
Veiga, Jesús
Figuerola, Jordi
Videvall, Elin
Martínez de la Puente, Josué
author Garrigós, Marta
author_facet Garrigós, Marta
García Ruiz, Olaya
Enkvist, Charlotte
García-López, María José
Moreno-Indias, Isabel
Ruiz-López, María José
Veiga, Jesús
Figuerola, Jordi
Videvall, Elin
Martínez de la Puente, Josué
author_role author
author2 García Ruiz, Olaya
Enkvist, Charlotte
García-López, María José
Moreno-Indias, Isabel
Ruiz-López, María José
Veiga, Jesús
Figuerola, Jordi
Videvall, Elin
Martínez de la Puente, Josué
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
European Commission
Ministerio de Universidades (España)
Instituto de Salud Carlos III
Swedish Research Council
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Avian malaria
Bacteria
Microbiome
Mosquitoes
Vector-borne pathogens
topic Avian malaria
Bacteria
Microbiome
Mosquitoes
Vector-borne pathogens
description Malaria parasites (Plasmodium spp.) are mosquito-borne parasites that infect humans and wildlife. Several studies support the role of mosquito microbiota as a major driver of Plasmodium transmission, although studies on wildlife malaria are typically neglected. Here, we used a 16S rRNA metabarcoding approach to assess whether the exposure to avian Plasmodium parasites affects the microbiota of their natural vector, Culex pipiens. Mosquitoes, captured in the field as larvae and grown in the laboratory, were allowed to feed on house sparrows (Passer domesticus) naturally infected with Plasmodium relictum (lineage SGS1) and uninfected birds. We analyzed the microbiota composition of the abdomens of individual mosquitoes and found 2,006 Amplicon Sequence Variants (ASVs). Culex pipiens' microbiota was dominated by bacteria of the genus Wolbachia, followed by the genera Stenotrophomonas and Faecalibacterium. We observed no difference in alpha nor beta diversity between mosquitoes that fed on Plasmodium-infected birds (exposed mosquitoes) and those that fed on uninfected birds (unexposed mosquitoes). However, exposed mosquitoes had a higher relative abundance of bacteria of the family Bacteroidaceae and the genus Bacteroides than the unexposed mosquitoes. Excluding the intracellular endosymbiont Wolbachia from the analyses, we obtained similar results, and also found a higher relative abundance of bacteria of the family Rikenellaceae in exposed mosquitoes. A pathway enrichment analysis based on KEGG annotations revealed that the bacterial community in exposed mosquitoes was enriched in pathways mainly related to biosynthesis and metabolism. Our results suggest that Cx. pipiens exposed to avian Plasmodium have slightly different microbiota composition, although further research is needed to establish the causality of these effects.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
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dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/405361
url http://hdl.handle.net/10261/405361
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Marta Garrigós, Olaya García-Ruiz, Charlotte Enkvist, María José García-López, Isabel Moreno-Indias, María José Ruiz-López, Jesús Veiga, Jordi Figuerola, Elin Videvall, Josué Martínez-de la Puente; 2025; 16S rRNA gene data of Culex pipiens females exposed and unexposed to Plasmodium relictum [Dataset]; Digital.CSIC; https://doi.org/10.20350/digitalCSIC/17367
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1038/s41598-025-22774-w
https://doi.org/10.1038/s41598-025-22774-w
Sí
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dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
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spelling Effects of avian Plasmodium exposure on the microbiota of Culex pipiensGarrigós, MartaGarcía Ruiz, OlayaEnkvist, CharlotteGarcía-López, María JoséMoreno-Indias, IsabelRuiz-López, María JoséVeiga, JesúsFiguerola, JordiVidevall, ElinMartínez de la Puente, JosuéAvian malariaBacteriaMicrobiomeMosquitoesVector-borne pathogensMalaria parasites (Plasmodium spp.) are mosquito-borne parasites that infect humans and wildlife. Several studies support the role of mosquito microbiota as a major driver of Plasmodium transmission, although studies on wildlife malaria are typically neglected. Here, we used a 16S rRNA metabarcoding approach to assess whether the exposure to avian Plasmodium parasites affects the microbiota of their natural vector, Culex pipiens. Mosquitoes, captured in the field as larvae and grown in the laboratory, were allowed to feed on house sparrows (Passer domesticus) naturally infected with Plasmodium relictum (lineage SGS1) and uninfected birds. We analyzed the microbiota composition of the abdomens of individual mosquitoes and found 2,006 Amplicon Sequence Variants (ASVs). Culex pipiens' microbiota was dominated by bacteria of the genus Wolbachia, followed by the genera Stenotrophomonas and Faecalibacterium. We observed no difference in alpha nor beta diversity between mosquitoes that fed on Plasmodium-infected birds (exposed mosquitoes) and those that fed on uninfected birds (unexposed mosquitoes). However, exposed mosquitoes had a higher relative abundance of bacteria of the family Bacteroidaceae and the genus Bacteroides than the unexposed mosquitoes. Excluding the intracellular endosymbiont Wolbachia from the analyses, we obtained similar results, and also found a higher relative abundance of bacteria of the family Rikenellaceae in exposed mosquitoes. A pathway enrichment analysis based on KEGG annotations revealed that the bacterial community in exposed mosquitoes was enriched in pathways mainly related to biosynthesis and metabolism. Our results suggest that Cx. pipiens exposed to avian Plasmodium have slightly different microbiota composition, although further research is needed to establish the causality of these effects.This work was supported by the MICIU/AEI/10.13039/501100011033 under grants PID2020-118205GB-I00, PID2021-123761OB-I00, and PID2023-151597NB-I00. MG, OGRuiz and JV were respectively financed by a FPI contract (PRE2021-098544), a FPU contract (FPU19/01925) and a Juan de la Cierva contract (FJC2021-048057-I) funded by MICIU/AEI/10.13039/501100011033 and the European Union NextGenerationEU/PRTR. MJRL was funded by the Agencia Estatal de Investigación (project PID2020-118921RJ-100/AEI/10.13039/501100011033) from the Spanish Ministry of Universities of Spain. MJGL was funded by the Agencia Estatal de Investigación (PTA2022-021854-I). IMI was supported by the “Miguel Servet Type II” program (CPII21/00013) of the ISCIII-Madrid (Spain), co-financed by the FEDER. EV was supported by the Swedish Research Council (2023–05026).Peer reviewedSpringer NatureMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European CommissionMinisterio de Universidades (España)Instituto de Salud Carlos IIISwedish Research CouncilConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/405361reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AEIinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2021-123761OB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-151597NB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-118921RJ-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PTA2022-021854-IMarta Garrigós, Olaya García-Ruiz, Charlotte Enkvist, María José García-López, Isabel Moreno-Indias, María José Ruiz-López, Jesús Veiga, Jordi Figuerola, Elin Videvall, Josué Martínez-de la Puente; 2025; 16S rRNA gene data of Culex pipiens females exposed and unexposed to Plasmodium relictum [Dataset]; Digital.CSIC; https://doi.org/10.20350/digitalCSIC/17367The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1038/s41598-025-22774-whttps://doi.org/10.1038/s41598-025-22774-wSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4053612026-05-22T06:33:51Z
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