Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction

[EN] Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria x anahassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network of defense mechanisms mediated by a diverse array of signaling molecules. In strawberry, resistance...

Descripción completa

Detalles Bibliográficos
Autores: Amil-Ruiz, F., Garrido-Gala, J., Blanco-Portales, R., Munoz-Merida, A., Trelles, O., de los Santos, B., Arroyo, F.T., Aguado-Puig, A., Romero, F., Mercado, J.A., Pliego-Alfaro, F., Munoz-Blanco, J., Caballero, J.L., Gadea Vacas, José|||0000-0002-3612-7914
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/140827
Acceso en línea:https://riunet.upv.es/handle/10251/140827
Access Level:acceso abierto
Palabra clave:Colletotrichum acutatum
Fragaria x ananassa
Quantification of gene expression
Salicylic and jasmonic acid
Strawberry defense response
BIOQUIMICA Y BIOLOGIA MOLECULAR
id ES_bacf26a5d08e8a7ea4c9e81887e6eaa3
oai_identifier_str oai:riunet.upv.es:10251/140827
network_acronym_str ES
network_name_str España
repository_id_str
spelling Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum InteractionAmil-Ruiz, F.Garrido-Gala, J.Blanco-Portales, R.Munoz-Merida, A.Trelles, O.de los Santos, B.Arroyo, F.T.Aguado-Puig, A.Romero, F.Mercado, J.A.Pliego-Alfaro, F.Munoz-Blanco, J.Caballero, J.L.Gadea Vacas, José|||0000-0002-3612-7914Colletotrichum acutatumFragaria x ananassaQuantification of gene expressionSalicylic and jasmonic acidStrawberry defense responseBIOQUIMICA Y BIOLOGIA MOLECULAR[EN] Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria x anahassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network of defense mechanisms mediated by a diverse array of signaling molecules. In strawberry, resistance to a variety of pathogens has been reported to be mostly polygenic and quantitatively inherited, making it difficult to associate molecular markers with disease resistance genes. Colletotrichum acutaturn spp. is a major strawberry pathogen, and completely resistant cultivars have not been reported. Moreover, strawberry defense network components and mechanisms remain largely unknown and poorly understood. Assessment of the strawberry response to C. acutatum included a global transcript analysis, and acidic hormones SA and JA measurements were analyzed after challenge with the pathogen. Induction of transcripts corresponding to the SA and JA signaling pathways and key genes controlling major steps within these defense pathways was detected. Accordingly, SA and JA accumulated in strawberry after infection. Contrastingly, induction of several important SA, JA, and oxidative stress-responsive defense genes, including FaPR1-1, FaLOX2, FaJAR1, FaPDF1, and FaGST1, was not detected, which suggests that specific branches in these defense pathways (those leading to FaPR1-2, FaPR2-1, FaPR2-2, FaAOS, FaPR5, and FaPR10) were activated. Our results reveal that specific aspects in SA and JA dependent signaling pathways are activated in strawberry upon interaction with C. acutatum. Certain described defense-associated transcripts related to these two known signaling pathways do not increase in abundance following infection. This finding suggests new insight into a specific putative molecular strategy for defense against this pathogen.Authors are grateful to Dr. JM Lopez-Aranda (IFAPA-Centro de Churriana) for providing micropropagated strawberry plants and to Nicolas Garcia-Caparros for technical assistance. Authors also want to thank Kevin M. Folta for his insightful comments on the paper. This work was supported by Junta de Andalucia, Spain [Proyectos de Excelencia P07-AGR-02482/P12-AGR-2174, and grants to Grupo-BIO278].Frontiers Media SAInstituto Universitario Mixto de Biología Molecular y Celular de PlantasDepartamento de BiotecnologíaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalJunta de AndalucíaRepositorio Institucional de la Universitat Politècnica de València Riunet20162016-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/140827reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengJunta de Andalucía http://dx.doi.org/10.13039/501100011011 P07-AGR-02482Junta de Andalucía http://dx.doi.org/10.13039/501100011011 AGR-217 Control De Enfermedades De Los Cultivosopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1408272026-06-13T07:49:27Z
dc.title.none.fl_str_mv Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction
title Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction
spellingShingle Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction
Amil-Ruiz, F.
Colletotrichum acutatum
Fragaria x ananassa
Quantification of gene expression
Salicylic and jasmonic acid
Strawberry defense response
BIOQUIMICA Y BIOLOGIA MOLECULAR
title_short Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction
title_full Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction
title_fullStr Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction
title_full_unstemmed Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction
title_sort Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction
dc.creator.none.fl_str_mv Amil-Ruiz, F.
Garrido-Gala, J.
Blanco-Portales, R.
Munoz-Merida, A.
Trelles, O.
de los Santos, B.
Arroyo, F.T.
Aguado-Puig, A.
Romero, F.
Mercado, J.A.
Pliego-Alfaro, F.
Munoz-Blanco, J.
Caballero, J.L.
Gadea Vacas, José|||0000-0002-3612-7914
author Amil-Ruiz, F.
author_facet Amil-Ruiz, F.
Garrido-Gala, J.
Blanco-Portales, R.
Munoz-Merida, A.
Trelles, O.
de los Santos, B.
Arroyo, F.T.
Aguado-Puig, A.
Romero, F.
Mercado, J.A.
Pliego-Alfaro, F.
Munoz-Blanco, J.
Caballero, J.L.
Gadea Vacas, José|||0000-0002-3612-7914
author_role author
author2 Garrido-Gala, J.
Blanco-Portales, R.
Munoz-Merida, A.
Trelles, O.
de los Santos, B.
Arroyo, F.T.
Aguado-Puig, A.
Romero, F.
Mercado, J.A.
Pliego-Alfaro, F.
Munoz-Blanco, J.
Caballero, J.L.
Gadea Vacas, José|||0000-0002-3612-7914
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Departamento de Biotecnología
Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
Junta de Andalucía
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Colletotrichum acutatum
Fragaria x ananassa
Quantification of gene expression
Salicylic and jasmonic acid
Strawberry defense response
BIOQUIMICA Y BIOLOGIA MOLECULAR
topic Colletotrichum acutatum
Fragaria x ananassa
Quantification of gene expression
Salicylic and jasmonic acid
Strawberry defense response
BIOQUIMICA Y BIOLOGIA MOLECULAR
description [EN] Understanding the nature of pathogen host interaction may help improve strawberry (Fragaria x anahassa) cultivars. Plant resistance to pathogenic agents usually operates through a complex network of defense mechanisms mediated by a diverse array of signaling molecules. In strawberry, resistance to a variety of pathogens has been reported to be mostly polygenic and quantitatively inherited, making it difficult to associate molecular markers with disease resistance genes. Colletotrichum acutaturn spp. is a major strawberry pathogen, and completely resistant cultivars have not been reported. Moreover, strawberry defense network components and mechanisms remain largely unknown and poorly understood. Assessment of the strawberry response to C. acutatum included a global transcript analysis, and acidic hormones SA and JA measurements were analyzed after challenge with the pathogen. Induction of transcripts corresponding to the SA and JA signaling pathways and key genes controlling major steps within these defense pathways was detected. Accordingly, SA and JA accumulated in strawberry after infection. Contrastingly, induction of several important SA, JA, and oxidative stress-responsive defense genes, including FaPR1-1, FaLOX2, FaJAR1, FaPDF1, and FaGST1, was not detected, which suggests that specific branches in these defense pathways (those leading to FaPR1-2, FaPR2-1, FaPR2-2, FaAOS, FaPR5, and FaPR10) were activated. Our results reveal that specific aspects in SA and JA dependent signaling pathways are activated in strawberry upon interaction with C. acutatum. Certain described defense-associated transcripts related to these two known signaling pathways do not increase in abundance following infection. This finding suggests new insight into a specific putative molecular strategy for defense against this pathogen.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/140827
url https://riunet.upv.es/handle/10251/140827
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Junta de Andalucía http://dx.doi.org/10.13039/501100011011 P07-AGR-02482
Junta de Andalucía http://dx.doi.org/10.13039/501100011011 AGR-217 Control De Enfermedades De Los Cultivos
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media SA
publisher.none.fl_str_mv Frontiers Media SA
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869417949877501952
score 15.300719

Similares en LA Referencia