Multi-target methodology for the screening of blood specimens in drug-facilitated sexual assault cases

The forensic investigation of drug-facilitated sexual assaults (DFSA) requires the toxicological examination of biological matrices to detect possible substances in victims. Whole blood is an important sample since it is the only one available for analysis in a considerable percentage of cases. A ne...

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Detalhes bibliográficos
Autores: Prego Meleiro, Pablo, Quintela Jorge, Óscar, Montalvo, Gemma, García-Ruiz, Carmen
Formato: artículo
Fecha de publicación:2019
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/117373
Acesso em linha:https://hdl.handle.net/20.500.14352/117373
Access Level:acceso abierto
Palavra-chave:615.4
615.9
Drug-facilitated sexual assault (DFSA)
Drug-facilitated crime (DFC)
Blood
Protein precipitation
Phospholipid removal
Multi.target screening
Farmacología (Farmacia)
Toxicología (Farmacia)
32 Ciencias Médicas
id ES_bacec8207df8fe42554bfe360ce7623c
oai_identifier_str oai:docta.ucm.es:20.500.14352/117373
network_acronym_str ES
network_name_str España
repository_id_str
spelling Multi-target methodology for the screening of blood specimens in drug-facilitated sexual assault casesPrego Meleiro, PabloQuintela Jorge, ÓscarMontalvo, GemmaGarcía-Ruiz, Carmen615.4615.9Drug-facilitated sexual assault (DFSA)Drug-facilitated crime (DFC)BloodProtein precipitationPhospholipid removalMulti.target screeningFarmacología (Farmacia)Toxicología (Farmacia)32 Ciencias MédicasThe forensic investigation of drug-facilitated sexual assaults (DFSA) requires the toxicological examination of biological matrices to detect possible substances in victims. Whole blood is an important sample since it is the only one available for analysis in a considerable percentage of cases. A new screening methodology was developed for the detection of 96 compounds related to DFSA in blood. Sample treatment was based on a procedure combining protein precipitation and phospholipid removal before HPLC-MS/MS analysis. The new method operates in scheduled multiple reaction monitoring (sMRM) mode and uses the hybrid operating of the third quadrupole as an ion trap. The blood samples preparation was studied comparing four different protein precipitation treatments and two widely used extraction techniques as solid phase extraction and liquid-liquid extraction. The analytical performance of the screening method was evaluated in terms of selectivity and limits of detection. Finally, successful results were obtained applying the new method to the analysis of fifteen samples from real cases of the National Institute of Toxicology and Forensic Sciences.ElsevierUniversidad Complutense de Madrid20192019-01-0120192019-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/117373reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengIUICP Not available IUICP-2016-003open accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1173732026-06-02T12:44:21Z
dc.title.none.fl_str_mv Multi-target methodology for the screening of blood specimens in drug-facilitated sexual assault cases
title Multi-target methodology for the screening of blood specimens in drug-facilitated sexual assault cases
spellingShingle Multi-target methodology for the screening of blood specimens in drug-facilitated sexual assault cases
Prego Meleiro, Pablo
615.4
615.9
Drug-facilitated sexual assault (DFSA)
Drug-facilitated crime (DFC)
Blood
Protein precipitation
Phospholipid removal
Multi.target screening
Farmacología (Farmacia)
Toxicología (Farmacia)
32 Ciencias Médicas
title_short Multi-target methodology for the screening of blood specimens in drug-facilitated sexual assault cases
title_full Multi-target methodology for the screening of blood specimens in drug-facilitated sexual assault cases
title_fullStr Multi-target methodology for the screening of blood specimens in drug-facilitated sexual assault cases
title_full_unstemmed Multi-target methodology for the screening of blood specimens in drug-facilitated sexual assault cases
title_sort Multi-target methodology for the screening of blood specimens in drug-facilitated sexual assault cases
dc.creator.none.fl_str_mv Prego Meleiro, Pablo
Quintela Jorge, Óscar
Montalvo, Gemma
García-Ruiz, Carmen
author Prego Meleiro, Pablo
author_facet Prego Meleiro, Pablo
Quintela Jorge, Óscar
Montalvo, Gemma
García-Ruiz, Carmen
author_role author
author2 Quintela Jorge, Óscar
Montalvo, Gemma
García-Ruiz, Carmen
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 615.4
615.9
Drug-facilitated sexual assault (DFSA)
Drug-facilitated crime (DFC)
Blood
Protein precipitation
Phospholipid removal
Multi.target screening
Farmacología (Farmacia)
Toxicología (Farmacia)
32 Ciencias Médicas
topic 615.4
615.9
Drug-facilitated sexual assault (DFSA)
Drug-facilitated crime (DFC)
Blood
Protein precipitation
Phospholipid removal
Multi.target screening
Farmacología (Farmacia)
Toxicología (Farmacia)
32 Ciencias Médicas
description The forensic investigation of drug-facilitated sexual assaults (DFSA) requires the toxicological examination of biological matrices to detect possible substances in victims. Whole blood is an important sample since it is the only one available for analysis in a considerable percentage of cases. A new screening methodology was developed for the detection of 96 compounds related to DFSA in blood. Sample treatment was based on a procedure combining protein precipitation and phospholipid removal before HPLC-MS/MS analysis. The new method operates in scheduled multiple reaction monitoring (sMRM) mode and uses the hybrid operating of the third quadrupole as an ion trap. The blood samples preparation was studied comparing four different protein precipitation treatments and two widely used extraction techniques as solid phase extraction and liquid-liquid extraction. The analytical performance of the screening method was evaluated in terms of selectivity and limits of detection. Finally, successful results were obtained applying the new method to the analysis of fifteen samples from real cases of the National Institute of Toxicology and Forensic Sciences.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01
2019
2019-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/117373
url https://hdl.handle.net/20.500.14352/117373
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv IUICP Not available IUICP-2016-003
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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