Bi-Reporter Vaccinia Virus for Tracking Viral Infections In Vitro and In Vivo

18 Pág. Centro de Biotecnología y Genómica de Plantas (CBGP)

Detalles Bibliográficos
Autores: Chiem, Kevin, Lorenzo, María M., Rangel-Moreno, Javier, Garcia-Hernandez, Maria De La Luz, Park, Jun-Gyu, Nogales, Aitor, Blasco, Rafael, Martínez-Sobrido, Luis
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/280605
Acceso en línea:http://hdl.handle.net/10261/280605
https://api.elsevier.com/content/abstract/scopus_id/85122740569
Access Level:acceso abierto
Palabra clave:GFP
NanoLuc
Scarlet
Bioluminescence
Fluorescence
In vitro
In vivo
In vivo imaging
Luciferase
Reporter genes
Vaccinia virus
id ES_ba9f09bbed71fa0d30d9e79501c3d02f
oai_identifier_str oai:digital.csic.es:10261/280605
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Bi-Reporter Vaccinia Virus for Tracking Viral Infections In Vitro and In Vivo
title Bi-Reporter Vaccinia Virus for Tracking Viral Infections In Vitro and In Vivo
spellingShingle Bi-Reporter Vaccinia Virus for Tracking Viral Infections In Vitro and In Vivo
Chiem, Kevin
GFP
NanoLuc
Scarlet
Bioluminescence
Fluorescence
In vitro
In vivo
In vivo imaging
Luciferase
Reporter genes
Vaccinia virus
title_short Bi-Reporter Vaccinia Virus for Tracking Viral Infections In Vitro and In Vivo
title_full Bi-Reporter Vaccinia Virus for Tracking Viral Infections In Vitro and In Vivo
title_fullStr Bi-Reporter Vaccinia Virus for Tracking Viral Infections In Vitro and In Vivo
title_full_unstemmed Bi-Reporter Vaccinia Virus for Tracking Viral Infections In Vitro and In Vivo
title_sort Bi-Reporter Vaccinia Virus for Tracking Viral Infections In Vitro and In Vivo
dc.creator.none.fl_str_mv Chiem, Kevin
Lorenzo, María M.
Rangel-Moreno, Javier
Garcia-Hernandez, Maria De La Luz
Park, Jun-Gyu
Nogales, Aitor
Blasco, Rafael
Martínez-Sobrido, Luis
author Chiem, Kevin
author_facet Chiem, Kevin
Lorenzo, María M.
Rangel-Moreno, Javier
Garcia-Hernandez, Maria De La Luz
Park, Jun-Gyu
Nogales, Aitor
Blasco, Rafael
Martínez-Sobrido, Luis
author_role author
author2 Lorenzo, María M.
Rangel-Moreno, Javier
Garcia-Hernandez, Maria De La Luz
Park, Jun-Gyu
Nogales, Aitor
Blasco, Rafael
Martínez-Sobrido, Luis
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Instituto de Salud Carlos III
Chiem, Kevin [0000-0002-3892-5944]
Lorenzo, María M. [0000-0001-7588-673X]
Rangel-Moreno, Javier [0000-0002-9738-1182]
Garcia-Hernandez, Maria De La Luz [0000-0001-9268-2464]
Nogales, Aitor [0000-0002-2424-7900]
Blasco, Rafael [0000-0002-8819-5767]
Martínez-Sobrido, Luis [0000-0001-7084-0804]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv GFP
NanoLuc
Scarlet
Bioluminescence
Fluorescence
In vitro
In vivo
In vivo imaging
Luciferase
Reporter genes
Vaccinia virus
topic GFP
NanoLuc
Scarlet
Bioluminescence
Fluorescence
In vitro
In vivo
In vivo imaging
Luciferase
Reporter genes
Vaccinia virus
description 18 Pág. Centro de Biotecnología y Genómica de Plantas (CBGP)
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/280605
https://api.elsevier.com/content/abstract/scopus_id/85122740569
url http://hdl.handle.net/10261/280605
https://api.elsevier.com/content/abstract/scopus_id/85122740569
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO//E-RTA2014-00006-C02-01
info:eu-repo/grantAgreement/MICIU//RTA2017-0066
Microbiology spectrum
https://doi.org/10.1128/Spectrum.01601-21
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869417933246038016
spelling Bi-Reporter Vaccinia Virus for Tracking Viral Infections In Vitro and In VivoChiem, KevinLorenzo, María M.Rangel-Moreno, JavierGarcia-Hernandez, Maria De La LuzPark, Jun-GyuNogales, AitorBlasco, RafaelMartínez-Sobrido, LuisGFPNanoLucScarletBioluminescenceFluorescenceIn vitroIn vivoIn vivo imagingLuciferaseReporter genesVaccinia virus18 Pág. Centro de Biotecnología y Genómica de Plantas (CBGP)Recombinant viruses expressing reporter genes allow visualization and quantification of viral infections and can be used as valid surrogates to identify the presence of the virus in infected cells and animal models. However, one of the limitations of recombinant viruses expressing reporter genes is the use of either fluorescent or luciferase proteins that are used alternatively for different purposes. Vaccinia virus (VV) is widely used as a viral vector, including recombinant (r)VV singly expressing either fluorescent or luciferase reporter genes that are useful for specific purposes. In this report, we engineered two novel rVV stably expressing both fluorescent (Scarlet or GFP) and luciferase (Nluc) reporter genes from different loci in the viral genome. In vitro, these bi-reporter-expressing rVV have similar growth kinetics and plaque phenotype than those of the parental WR VV isolate. In vivo, rVV Nluc/Scarlet and rVV Nluc/GFP effectively infected mice and were easily detected using in vivo imaging systems (IVIS) and ex vivo in the lungs from infected mice. Importantly, we used these bi-reporter-expressing rVV to assess viral pathogenesis, infiltration of immune cells in the lungs, and to directly identify the different subsets of cells infected by VV in the absence of antibody staining. Collectively, these rVV expressing two reporter genes open the feasibility to study the biology of viral infections in vitro and in vivo, including host-pathogen interactions and dynamics or tropism of viral infections. IMPORTANCE Despite the eradication of variola virus (VARV), the causative agent of smallpox, poxviruses still represent an important threat to human health due to their possible use as bioterrorism agents and the emergence of zoonotic poxvirus diseases. Recombinant vaccinia viruses (rVV) expressing easily traceable fluorescent or luciferase reporter genes have significantly contributed to the progress of poxvirus research. However, rVV expressing one marker gene have several constraints for in vitro and in vivo studies, since both fluorescent and luciferase proteins impose certain limitations for specific applications. To overcome these limitations, we generated optimized rVV stably expressing both fluorescent (Scarlet or GFP) and luciferase (Nluc) reporter genes to easily track viral infection in vitro and in vivo. This new generation of double reporter-expressing rVV represent an excellent option to study viral infection dynamics in cultured cells and validated animal models of infection.This work was supported by grants E-RTA2014-00006, RTA2017-0066 from Ministerio de Economía y Competitividad and Ministerio de Ciencia, Innovación y Universidades as part of the Plan Estatal de Investigación Científica, Desarrollo e Innovación Tecnológica, and grant COV20-00901 from Instituto de Salud Carlos III (ISCIII)Peer reviewedAmerican Society for MicrobiologyMinisterio de Economía y Competitividad (España)Ministerio de Ciencia, Innovación y Universidades (España)Instituto de Salud Carlos IIIChiem, Kevin [0000-0002-3892-5944]Lorenzo, María M. [0000-0001-7588-673X]Rangel-Moreno, Javier [0000-0002-9738-1182]Garcia-Hernandez, Maria De La Luz [0000-0001-9268-2464]Nogales, Aitor [0000-0002-2424-7900]Blasco, Rafael [0000-0002-8819-5767]Martínez-Sobrido, Luis [0000-0001-7084-0804]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/280605https://api.elsevier.com/content/abstract/scopus_id/85122740569reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//E-RTA2014-00006-C02-01info:eu-repo/grantAgreement/MICIU//RTA2017-0066Microbiology spectrumhttps://doi.org/10.1128/Spectrum.01601-21Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2806052026-05-22T06:33:51Z
score 15,811543

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