Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep
[EN]Vaccination is the most effective tool for paratuberculosis control. Currently, available vaccines prevent the progression of clinical disease in most animals but do not fully protect them against infection and induce the formation of an injection site granuloma. The precise mechanisms that oper...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad de León |
| Repositorio: | BULERIA. Repositorio Institucional de la Universidad de León |
| OAI Identifier: | oai:buleria.unileon.es:10612/25803 |
| Acceso en línea: | https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1284902/full https://hdl.handle.net/10612/25803 |
| Access Level: | acceso abierto |
| Palabra clave: | Inmunología Sanidad animal Veterinaria Adjuvants Paratuberculosis Vaccine injection-site Granuloma Gudair Silirum 2412.10 Vacunas 3109.03 Inmunología 3104.07 Ovinos |
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Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheepCriado Boyero, MiguelReyes Ávila, Luis ErnestoGarcía Marín, Juan FranciscoGutiérrez Expósito, DanielZapico Sánchez, DavidEspinosa Cerrato, JoséPérez Pérez, ValentínInmunologíaSanidad animalVeterinariaAdjuvantsParatuberculosisVaccine injection-siteGranulomaGudairSilirum2412.10 Vacunas3109.03 Inmunología3104.07 Ovinos[EN]Vaccination is the most effective tool for paratuberculosis control. Currently, available vaccines prevent the progression of clinical disease in most animals but do not fully protect them against infection and induce the formation of an injection site granuloma. The precise mechanisms that operate in response to vaccination and granuloma development, as well as the effect that adjuvants could trigger, have not been fully investigated. Therefore, this study aimed to investigate the injection site granulomas induced by two inactivated paratuberculosis vaccines, which differ in the adjuvant employed. Two groups of 45-day-old lambs were immunized with two commercially available vaccines—one (n = 4) with Gudair® and the other (n = 4) with Silirum®. A third group (n = 4) was not vaccinated and served as control. The peripheral humoral response was assessed throughout the study by a commercial anti-Mycobacterium avium subspecies paratuberculosis (Map) antibody indirect ELISA, and the cellular immune response was assessed similarly by the IFN-γ release and comparative intradermal tests. The injection site granulomas were measured during the experiment and sampled at 75 days post-vaccination (dpv) when the animals were euthanized. The tissue damage, antigen and adjuvant distribution, and the presence and amount of immune cells were then determined and assessed by immunohistochemical methods. Antibodies against Map antigens; a general macrophage marker (Iba1), M1 (iNOS), and M2 (CD204) macrophages; T (CD3), B (CD20), and γδ T lymphocytes, proteins MHC-II and NRAMP1, and cytokines IL-4, IL-10, TNF, and IFN-γ were employed. Silirum® elicited a stronger peripheral cellular immune response than Gudair®, while the latter induced larger granulomas and more tissue damage at the site of injection. Additionally, adjuvant and Map antigen distribution throughout the granulomatous inflammatory infiltrate, as well as the NRAMP1 cell expression, which is linked to antigen phagocytosis, were highly irregular. In Silirum® induced granulomas, a higher number of MHC-II and TNF-expressing cells and a lower number of M2 macrophages suggested an improved antigen presentation, which could be due to the better antigen distribution and reduced tissue damage induced by this vaccine.SIThe author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was funded by the national project RTI2018–099496-B-I00 and PID2021–125807OB-C21, from the “Agencia Estatal de Investigación”, of the Spanish Ministry of Science and Innovation. MC acknowledges the receipt of a pre-doctoral grant (PRE2019–087309) from MCIN/AEI/ 10.13039/501100011033 and FSE “El FSE invierte en tu futuro” and DZ of a predoctoral contract of the University of León. Zendal (Porriño, Spain), supplied Gudair ® and Silirum ® vaccines.Frontiers MediaSanidad AnimalFacultad de Veterinaria2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1284902/fullhttps://hdl.handle.net/10612/25803reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RTI2018–099496-B-I00info:eu-repo/grantAgreement/AEI/Programa Estatal para Impulsar la Investigación Científico-Técnica y su Transferencia/PID2021–125807OB-C21http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/258032026-06-24T12:43:27Z |
| dc.title.none.fl_str_mv |
Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep |
| title |
Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep |
| spellingShingle |
Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep Criado Boyero, Miguel Inmunología Sanidad animal Veterinaria Adjuvants Paratuberculosis Vaccine injection-site Granuloma Gudair Silirum 2412.10 Vacunas 3109.03 Inmunología 3104.07 Ovinos |
| title_short |
Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep |
| title_full |
Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep |
| title_fullStr |
Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep |
| title_full_unstemmed |
Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep |
| title_sort |
Adjuvants influence the immune cell populations present at the injection site granuloma induced by whole-cell inactivated paratuberculosis vaccines in sheep |
| dc.creator.none.fl_str_mv |
Criado Boyero, Miguel Reyes Ávila, Luis Ernesto García Marín, Juan Francisco Gutiérrez Expósito, Daniel Zapico Sánchez, David Espinosa Cerrato, José Pérez Pérez, Valentín |
| author |
Criado Boyero, Miguel |
| author_facet |
Criado Boyero, Miguel Reyes Ávila, Luis Ernesto García Marín, Juan Francisco Gutiérrez Expósito, Daniel Zapico Sánchez, David Espinosa Cerrato, José Pérez Pérez, Valentín |
| author_role |
author |
| author2 |
Reyes Ávila, Luis Ernesto García Marín, Juan Francisco Gutiérrez Expósito, Daniel Zapico Sánchez, David Espinosa Cerrato, José Pérez Pérez, Valentín |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Sanidad Animal Facultad de Veterinaria |
| dc.subject.none.fl_str_mv |
Inmunología Sanidad animal Veterinaria Adjuvants Paratuberculosis Vaccine injection-site Granuloma Gudair Silirum 2412.10 Vacunas 3109.03 Inmunología 3104.07 Ovinos |
| topic |
Inmunología Sanidad animal Veterinaria Adjuvants Paratuberculosis Vaccine injection-site Granuloma Gudair Silirum 2412.10 Vacunas 3109.03 Inmunología 3104.07 Ovinos |
| description |
[EN]Vaccination is the most effective tool for paratuberculosis control. Currently, available vaccines prevent the progression of clinical disease in most animals but do not fully protect them against infection and induce the formation of an injection site granuloma. The precise mechanisms that operate in response to vaccination and granuloma development, as well as the effect that adjuvants could trigger, have not been fully investigated. Therefore, this study aimed to investigate the injection site granulomas induced by two inactivated paratuberculosis vaccines, which differ in the adjuvant employed. Two groups of 45-day-old lambs were immunized with two commercially available vaccines—one (n = 4) with Gudair® and the other (n = 4) with Silirum®. A third group (n = 4) was not vaccinated and served as control. The peripheral humoral response was assessed throughout the study by a commercial anti-Mycobacterium avium subspecies paratuberculosis (Map) antibody indirect ELISA, and the cellular immune response was assessed similarly by the IFN-γ release and comparative intradermal tests. The injection site granulomas were measured during the experiment and sampled at 75 days post-vaccination (dpv) when the animals were euthanized. The tissue damage, antigen and adjuvant distribution, and the presence and amount of immune cells were then determined and assessed by immunohistochemical methods. Antibodies against Map antigens; a general macrophage marker (Iba1), M1 (iNOS), and M2 (CD204) macrophages; T (CD3), B (CD20), and γδ T lymphocytes, proteins MHC-II and NRAMP1, and cytokines IL-4, IL-10, TNF, and IFN-γ were employed. Silirum® elicited a stronger peripheral cellular immune response than Gudair®, while the latter induced larger granulomas and more tissue damage at the site of injection. Additionally, adjuvant and Map antigen distribution throughout the granulomatous inflammatory infiltrate, as well as the NRAMP1 cell expression, which is linked to antigen phagocytosis, were highly irregular. In Silirum® induced granulomas, a higher number of MHC-II and TNF-expressing cells and a lower number of M2 macrophages suggested an improved antigen presentation, which could be due to the better antigen distribution and reduced tissue damage induced by this vaccine. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1284902/full https://hdl.handle.net/10612/25803 |
| url |
https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1284902/full https://hdl.handle.net/10612/25803 |
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Inglés |
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Inglés |
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info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RTI2018–099496-B-I00 info:eu-repo/grantAgreement/AEI/Programa Estatal para Impulsar la Investigación Científico-Técnica y su Transferencia/PID2021–125807OB-C21 |
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http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by/4.0/ |
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Frontiers Media |
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Frontiers Media |
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