The NS segment of H5N1 avian influenza viruses (AIV) enhances the virulence of an H7N1 AIV in chickens

Some outbreaks involving highly pathogenic avian influenza viruses (HPAIV) of subtypes H5 and H7 were caused by avian-to-human transmissions. In nature, different influenza A viruses can reassort leading to new viruses with new characteristics. We decided to investigate the impact that the NS-segmen...

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Autores: Serra Gironella, Joan, Busquets, Núria, Ballester Devis, Maria, Chaves, Aida J, Rivas, Raquel, Dolz, Roser, Wang, Zhongfang, Pleschka, Stephan, Majo, Natalia, Rodriguez, Fernando, Darji, Ayub
Tipo de recurso: artículo
Fecha de publicación:2014
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/3218
Acceso en línea:http://hdl.handle.net/20.500.12327/3218
https://doi.org/10.1186/1297-9716-45-7
Access Level:acceso abierto
Palabra clave:575
619
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spelling The NS segment of H5N1 avian influenza viruses (AIV) enhances the virulence of an H7N1 AIV in chickensSerra Gironella, JoanBusquets, NúriaBallester Devis, MariaChaves, Aida JRivas, RaquelDolz, RoserWang, ZhongfangPleschka, StephanMajo, NataliaRodriguez, FernandoDarji, Ayub575619Some outbreaks involving highly pathogenic avian influenza viruses (HPAIV) of subtypes H5 and H7 were caused by avian-to-human transmissions. In nature, different influenza A viruses can reassort leading to new viruses with new characteristics. We decided to investigate the impact that the NS-segment of H5 HPAIV would have on viral pathogenicity of a classical avian H7 HPAIV in poultry, a natural host. We focussed this study based on our previous work that demonstrated that single reassortment of the NS-segment from an H5 HPAIV into an H7 HPAIV changes the ability of the virus to replicate in mammalian hosts. Our present data show that two different H7-viruses containing an NS-segment from H5–types (FPV NS GD or FPV NS VN) show an overall highly pathogenic phenotype compared with the wild type H7–virus (FPV), as characterized by higher viral shedding and earlier manifestation of clinical signs. Correlating with the latter, higher amounts of IFN-β mRNA were detected in the blood of NS-reassortant infected birds, 48 h post-infection (pi). Although lymphopenia was detected in chickens from all IVinfected groups, also 48 h pi those animals challenged with NS-reassortant viruses showed an increase of peripheral monocyte/macrophage-like cells expressing high levels of IL-1β, as determined by flow cytometry. Taken together, these findings highlight the importance of the NS-segment in viral pathogenicity which is directly involved in triggering antiviral and pro-inflammatory cytokines found during HPAIV pathogenesis in chickens.info:eu-repo/semantics/publishedVersionBMCProducció AnimalGenètica i Millora AnimalSanitat Animal2014info:eu-repo/semantics/article11http://hdl.handle.net/20.500.12327/3218https://doi.org/10.1186/1297-9716-45-7reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVeterinary ResearchMEC/ /AGL2007-60434/ES/PAPEL DE LA INMUNIDAD INNATA DEL HOSPEDADOR EN LA PROTECCION Y PATOGENIA DE LA INFECCION POR EL VIRUS DE INFLUENZA AVIAR/GANMICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-22229 -C03-01/ES/CARACTERIZACION DE MECANISMOS IMPLICADOS EN PROTECCION FRENTE AL VIRUS DE LA PESTE PORCINA AFRICANA: DESARROLLO DE VACUNA FRENTE AL VPPA/Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/32182026-05-29T05:05:01Z
dc.title.none.fl_str_mv The NS segment of H5N1 avian influenza viruses (AIV) enhances the virulence of an H7N1 AIV in chickens
title The NS segment of H5N1 avian influenza viruses (AIV) enhances the virulence of an H7N1 AIV in chickens
spellingShingle The NS segment of H5N1 avian influenza viruses (AIV) enhances the virulence of an H7N1 AIV in chickens
Serra Gironella, Joan
575
619
title_short The NS segment of H5N1 avian influenza viruses (AIV) enhances the virulence of an H7N1 AIV in chickens
title_full The NS segment of H5N1 avian influenza viruses (AIV) enhances the virulence of an H7N1 AIV in chickens
title_fullStr The NS segment of H5N1 avian influenza viruses (AIV) enhances the virulence of an H7N1 AIV in chickens
title_full_unstemmed The NS segment of H5N1 avian influenza viruses (AIV) enhances the virulence of an H7N1 AIV in chickens
title_sort The NS segment of H5N1 avian influenza viruses (AIV) enhances the virulence of an H7N1 AIV in chickens
dc.creator.none.fl_str_mv Serra Gironella, Joan
Busquets, Núria
Ballester Devis, Maria
Chaves, Aida J
Rivas, Raquel
Dolz, Roser
Wang, Zhongfang
Pleschka, Stephan
Majo, Natalia
Rodriguez, Fernando
Darji, Ayub
author Serra Gironella, Joan
author_facet Serra Gironella, Joan
Busquets, Núria
Ballester Devis, Maria
Chaves, Aida J
Rivas, Raquel
Dolz, Roser
Wang, Zhongfang
Pleschka, Stephan
Majo, Natalia
Rodriguez, Fernando
Darji, Ayub
author_role author
author2 Busquets, Núria
Ballester Devis, Maria
Chaves, Aida J
Rivas, Raquel
Dolz, Roser
Wang, Zhongfang
Pleschka, Stephan
Majo, Natalia
Rodriguez, Fernando
Darji, Ayub
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Genètica i Millora Animal
Sanitat Animal
dc.subject.none.fl_str_mv 575
619
topic 575
619
description Some outbreaks involving highly pathogenic avian influenza viruses (HPAIV) of subtypes H5 and H7 were caused by avian-to-human transmissions. In nature, different influenza A viruses can reassort leading to new viruses with new characteristics. We decided to investigate the impact that the NS-segment of H5 HPAIV would have on viral pathogenicity of a classical avian H7 HPAIV in poultry, a natural host. We focussed this study based on our previous work that demonstrated that single reassortment of the NS-segment from an H5 HPAIV into an H7 HPAIV changes the ability of the virus to replicate in mammalian hosts. Our present data show that two different H7-viruses containing an NS-segment from H5–types (FPV NS GD or FPV NS VN) show an overall highly pathogenic phenotype compared with the wild type H7–virus (FPV), as characterized by higher viral shedding and earlier manifestation of clinical signs. Correlating with the latter, higher amounts of IFN-β mRNA were detected in the blood of NS-reassortant infected birds, 48 h post-infection (pi). Although lymphopenia was detected in chickens from all IVinfected groups, also 48 h pi those animals challenged with NS-reassortant viruses showed an increase of peripheral monocyte/macrophage-like cells expressing high levels of IL-1β, as determined by flow cytometry. Taken together, these findings highlight the importance of the NS-segment in viral pathogenicity which is directly involved in triggering antiviral and pro-inflammatory cytokines found during HPAIV pathogenesis in chickens.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/3218
https://doi.org/10.1186/1297-9716-45-7
url http://hdl.handle.net/20.500.12327/3218
https://doi.org/10.1186/1297-9716-45-7
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Veterinary Research
MEC/ /AGL2007-60434/ES/PAPEL DE LA INMUNIDAD INNATA DEL HOSPEDADOR EN LA PROTECCION Y PATOGENIA DE LA INFECCION POR EL VIRUS DE INFLUENZA AVIAR/GAN
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-22229 -C03-01/ES/CARACTERIZACION DE MECANISMOS IMPLICADOS EN PROTECCION FRENTE AL VIRUS DE LA PESTE PORCINA AFRICANA: DESARROLLO DE VACUNA FRENTE AL VPPA/
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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