Characterization of the composition and immunoregulatory activity of wheat cell culture-derived polysaccharides

Plant polysaccharides can exert immunomodulatory activities. In this study we provided chemical characterization of wheat cell culture-derived polysaccharides (WCCPS) and assessed their capacity to modulate inflammatory responses in mouse macrophages. The total sample (T-010) contained arabinogalact...

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Autores: Murtazina, Alima, Rodríguez-Martínez, Pol, Crawshaw, Dylan J., Caelles Franch, Carme, Tarabayeva, Anel, Bitanova, Elmira, Ibragimova, Nadezhda, Mikshina, Polina, Gorshkova, Tatyana, McDougall, Gordon J., Boulaiz, Houria, Bishimbayeva, Nazira, Valledor Fernández, Annabel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:dnet:recercat____::5e8b7b8ffb760cdff468fd1901ed2533
Acceso en línea:https://hdl.handle.net/2445/229557
Access Level:acceso abierto
Palabra clave:Immunologia
Polisacàrids
Plantes
Immunology
Polysaccharides
Plants
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spelling Characterization of the composition and immunoregulatory activity of wheat cell culture-derived polysaccharidesMurtazina, AlimaRodríguez-Martínez, PolCrawshaw, Dylan J.Caelles Franch, CarmeTarabayeva, AnelBitanova, ElmiraIbragimova, NadezhdaMikshina, PolinaGorshkova, TatyanaMcDougall, Gordon J.Boulaiz, HouriaBishimbayeva, NaziraValledor Fernández, AnnabelImmunologiaPolisacàridsPlantesImmunologyPolysaccharidesPlantsPlant polysaccharides can exert immunomodulatory activities. In this study we provided chemical characterization of wheat cell culture-derived polysaccharides (WCCPS) and assessed their capacity to modulate inflammatory responses in mouse macrophages. The total sample (T-010) contained arabinogalactans, arabinans, glucans and xyloglucans. Fractionation by anion-exchange chromatography rendered a bound acidic fraction (B-010) and an unbound neutral fraction (UB-010). The B-010 fraction was enriched in arabinogalactans and arabinans, with some galactans, homogalacturonans, and arabinoxylans. The neutral UB-010 fraction was composed of glucans and xyloglucans. None of the WCCPS preparations triggered cytokine production on their own, but each potentiated different macrophage responses to bacterial lipopolysaccharide (LPS). The total WCCPS in T-010 increased LPS-induced tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 secretion, whereas the acidic arabinogalactan-rich fraction B-010 boosted IL-6 release and selectively upregulated nitric oxide synthase 2 (Nos2) and cholesterol 25-hydroxylase (Ch25h) expression in response to LPS. In contrast, the neutral UB-010 fraction enhanced IL-6 levels and induced Nos2 expression without altering Ch25h expression. These results suggest that WCCPS can modulate distinct aspects of the inflammatory response, with their effects shaped by their composition and structural features. Future research will focus on elucidating the molecular mechanisms underlying the immunomodulatory activity of WCCPS.MDPI2026202620262026info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion19 p.application/pdfhttps://hdl.handle.net/2445/229557https://hdl.handle.net/2445/229557reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.3390/molecules31091540Molecules, 2026, vol. 9, num.31https://doi.org/10.3390/molecules31091540cc-by (c) Murtazina A. et al., 2026http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:dnet:recercat____::5e8b7b8ffb760cdff468fd1901ed25332026-05-29T05:05:01Z
dc.title.none.fl_str_mv Characterization of the composition and immunoregulatory activity of wheat cell culture-derived polysaccharides
title Characterization of the composition and immunoregulatory activity of wheat cell culture-derived polysaccharides
spellingShingle Characterization of the composition and immunoregulatory activity of wheat cell culture-derived polysaccharides
Murtazina, Alima
Immunologia
Polisacàrids
Plantes
Immunology
Polysaccharides
Plants
title_short Characterization of the composition and immunoregulatory activity of wheat cell culture-derived polysaccharides
title_full Characterization of the composition and immunoregulatory activity of wheat cell culture-derived polysaccharides
title_fullStr Characterization of the composition and immunoregulatory activity of wheat cell culture-derived polysaccharides
title_full_unstemmed Characterization of the composition and immunoregulatory activity of wheat cell culture-derived polysaccharides
title_sort Characterization of the composition and immunoregulatory activity of wheat cell culture-derived polysaccharides
dc.creator.none.fl_str_mv Murtazina, Alima
Rodríguez-Martínez, Pol
Crawshaw, Dylan J.
Caelles Franch, Carme
Tarabayeva, Anel
Bitanova, Elmira
Ibragimova, Nadezhda
Mikshina, Polina
Gorshkova, Tatyana
McDougall, Gordon J.
Boulaiz, Houria
Bishimbayeva, Nazira
Valledor Fernández, Annabel
author Murtazina, Alima
author_facet Murtazina, Alima
Rodríguez-Martínez, Pol
Crawshaw, Dylan J.
Caelles Franch, Carme
Tarabayeva, Anel
Bitanova, Elmira
Ibragimova, Nadezhda
Mikshina, Polina
Gorshkova, Tatyana
McDougall, Gordon J.
Boulaiz, Houria
Bishimbayeva, Nazira
Valledor Fernández, Annabel
author_role author
author2 Rodríguez-Martínez, Pol
Crawshaw, Dylan J.
Caelles Franch, Carme
Tarabayeva, Anel
Bitanova, Elmira
Ibragimova, Nadezhda
Mikshina, Polina
Gorshkova, Tatyana
McDougall, Gordon J.
Boulaiz, Houria
Bishimbayeva, Nazira
Valledor Fernández, Annabel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Immunologia
Polisacàrids
Plantes
Immunology
Polysaccharides
Plants
topic Immunologia
Polisacàrids
Plantes
Immunology
Polysaccharides
Plants
description Plant polysaccharides can exert immunomodulatory activities. In this study we provided chemical characterization of wheat cell culture-derived polysaccharides (WCCPS) and assessed their capacity to modulate inflammatory responses in mouse macrophages. The total sample (T-010) contained arabinogalactans, arabinans, glucans and xyloglucans. Fractionation by anion-exchange chromatography rendered a bound acidic fraction (B-010) and an unbound neutral fraction (UB-010). The B-010 fraction was enriched in arabinogalactans and arabinans, with some galactans, homogalacturonans, and arabinoxylans. The neutral UB-010 fraction was composed of glucans and xyloglucans. None of the WCCPS preparations triggered cytokine production on their own, but each potentiated different macrophage responses to bacterial lipopolysaccharide (LPS). The total WCCPS in T-010 increased LPS-induced tumor necrosis factor-alpha (TNF-α) and interleukin (IL)-6 secretion, whereas the acidic arabinogalactan-rich fraction B-010 boosted IL-6 release and selectively upregulated nitric oxide synthase 2 (Nos2) and cholesterol 25-hydroxylase (Ch25h) expression in response to LPS. In contrast, the neutral UB-010 fraction enhanced IL-6 levels and induced Nos2 expression without altering Ch25h expression. These results suggest that WCCPS can modulate distinct aspects of the inflammatory response, with their effects shaped by their composition and structural features. Future research will focus on elucidating the molecular mechanisms underlying the immunomodulatory activity of WCCPS.
publishDate 2026
dc.date.none.fl_str_mv 2026
2026
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/229557
https://hdl.handle.net/2445/229557
url https://hdl.handle.net/2445/229557
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/molecules31091540
Molecules, 2026, vol. 9, num.31
https://doi.org/10.3390/molecules31091540
dc.rights.none.fl_str_mv cc-by (c) Murtazina A. et al., 2026
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Murtazina A. et al., 2026
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 19 p.
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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