Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation

This study describes the preparation and evaluation of two formulations, a hydrogel and a nanostructured system, containing ketorolac tromethamine as an anti-inflammatory agent for the local therapy against the inflammatory process derived from the surgical excision of Condyloma acuminata. Both form...

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Detalles Bibliográficos
Autores: El Moussaoui, Salima, Abo-Horan, Ismael, Halbaut, Lyda, Alonso, Cristina, Coderch Negra, M. Luisa, Garduño-Ramírez, María Luisa, Clares, Beatriz, Soriano, José Luis, Calpena, Ana Cristina, Fernández-Campos, Francisco, Mallandrich, Mireia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/255581
Acceso en línea:http://hdl.handle.net/10261/255581
https://api.elsevier.com/content/abstract/scopus_id/85117926505
Access Level:acceso abierto
Palabra clave:Condyloma acuminata
PLGA nanoparticles
Anti-inflammatory
Chitosan gel
Ketorolac tromethamine
Topical delivery
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spelling Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-AblationEl Moussaoui, SalimaAbo-Horan, IsmaelHalbaut, LydaAlonso, CristinaCoderch Negra, M. LuisaGarduño-Ramírez, María LuisaClares, BeatrizSoriano, José LuisCalpena, Ana CristinaFernández-Campos, FranciscoMallandrich, MireiaCondyloma acuminataPLGA nanoparticlesAnti-inflammatoryChitosan gelKetorolac tromethamineTopical deliveryThis study describes the preparation and evaluation of two formulations, a hydrogel and a nanostructured system, containing ketorolac tromethamine as an anti-inflammatory agent for the local therapy against the inflammatory process derived from the surgical excision of Condyloma acuminata. Both formulations were physicochemically characterized. In vitro release profiles show that the nanoparticles release 92% ± 2.3 of the total ketorolac tromethamine encapsulated, while the chitosan gel releases 18.6% ± 0.2. The ex vivo permeation and distribution through human skin were also assayed and was observed how the main amount of ketorolac tromethamine is retained in the epidermis. In vivo studies were accomplished to evaluate the anti-inflammatory efficacy in mice which also involved the histological analysis to confirm the in vivo results. The nanoparticles present a significantly higher anti-inflammatory efficacy than chitosan gel. The tolerability of developed formulations was assessed by monitoring the biomechanical properties of the skin before and after application of both formulations. No statistical differences in trans-epidermal water loss and skin hydration with respect to the basal values were observed and the formulations exhibited higher anti-inflammatory activity compared to a reference ketotorlac tromethamine solution. Therefore, it can be concluded that both formulations can be proposed as outstanding candidates for offering a local anti-inflammatory therapeutical tool with potential clinical application.The authors want to acknowledge Maria Luisa García for the PLGA donation and Noelia Pérez-González for their technical support.Peer reviewedMultidisciplinary Digital Publishing InstituteConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/255581https://api.elsevier.com/content/abstract/scopus_id/85117926505reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésPharmaceuticshttps://doi.org/10.3390/pharmaceutics13111784Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2555812026-05-22T06:33:51Z
dc.title.none.fl_str_mv Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
title Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
spellingShingle Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
El Moussaoui, Salima
Condyloma acuminata
PLGA nanoparticles
Anti-inflammatory
Chitosan gel
Ketorolac tromethamine
Topical delivery
title_short Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
title_full Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
title_fullStr Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
title_full_unstemmed Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
title_sort Polymeric Nanoparticles and Chitosan Gel Loading Ketorolac Tromethamine to Alleviate Pain Associated with Condyloma Acuminata during the Pre- and Post-Ablation
dc.creator.none.fl_str_mv El Moussaoui, Salima
Abo-Horan, Ismael
Halbaut, Lyda
Alonso, Cristina
Coderch Negra, M. Luisa
Garduño-Ramírez, María Luisa
Clares, Beatriz
Soriano, José Luis
Calpena, Ana Cristina
Fernández-Campos, Francisco
Mallandrich, Mireia
author El Moussaoui, Salima
author_facet El Moussaoui, Salima
Abo-Horan, Ismael
Halbaut, Lyda
Alonso, Cristina
Coderch Negra, M. Luisa
Garduño-Ramírez, María Luisa
Clares, Beatriz
Soriano, José Luis
Calpena, Ana Cristina
Fernández-Campos, Francisco
Mallandrich, Mireia
author_role author
author2 Abo-Horan, Ismael
Halbaut, Lyda
Alonso, Cristina
Coderch Negra, M. Luisa
Garduño-Ramírez, María Luisa
Clares, Beatriz
Soriano, José Luis
Calpena, Ana Cristina
Fernández-Campos, Francisco
Mallandrich, Mireia
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Condyloma acuminata
PLGA nanoparticles
Anti-inflammatory
Chitosan gel
Ketorolac tromethamine
Topical delivery
topic Condyloma acuminata
PLGA nanoparticles
Anti-inflammatory
Chitosan gel
Ketorolac tromethamine
Topical delivery
description This study describes the preparation and evaluation of two formulations, a hydrogel and a nanostructured system, containing ketorolac tromethamine as an anti-inflammatory agent for the local therapy against the inflammatory process derived from the surgical excision of Condyloma acuminata. Both formulations were physicochemically characterized. In vitro release profiles show that the nanoparticles release 92% ± 2.3 of the total ketorolac tromethamine encapsulated, while the chitosan gel releases 18.6% ± 0.2. The ex vivo permeation and distribution through human skin were also assayed and was observed how the main amount of ketorolac tromethamine is retained in the epidermis. In vivo studies were accomplished to evaluate the anti-inflammatory efficacy in mice which also involved the histological analysis to confirm the in vivo results. The nanoparticles present a significantly higher anti-inflammatory efficacy than chitosan gel. The tolerability of developed formulations was assessed by monitoring the biomechanical properties of the skin before and after application of both formulations. No statistical differences in trans-epidermal water loss and skin hydration with respect to the basal values were observed and the formulations exhibited higher anti-inflammatory activity compared to a reference ketotorlac tromethamine solution. Therefore, it can be concluded that both formulations can be proposed as outstanding candidates for offering a local anti-inflammatory therapeutical tool with potential clinical application.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/255581
https://api.elsevier.com/content/abstract/scopus_id/85117926505
url http://hdl.handle.net/10261/255581
https://api.elsevier.com/content/abstract/scopus_id/85117926505
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Pharmaceutics
https://doi.org/10.3390/pharmaceutics13111784
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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