Temperature-mediated biosynthesis of the phytotoxin phaseolotoxin by Pseudomonas syringae pv. phaseolicola depends on the autoregulated expression of the phtABC genes

Pseudomonas syringae pv. phaseolicola produces phaseolotoxin in a temperature dependent manner, being optimally synthesized between 18ºC and 20ºC, while no detectable amounts are present above 28ºC. The Pht cluster, involved in the biosynthesis of phaseolotoxin, contains 23 genes that are organized...

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Autores: Aguilera, Selene, Álvarez Morales, Ariel, Murillo Martínez, Jesús, Hernández Flores, José Luis, Bravo, Jaime, Torre Zavala, Susana de la
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/30538
Acceso en línea:https://hdl.handle.net/2454/30538
Access Level:acceso abierto
Palabra clave:Pseudomonas syringae pv. Phaseolicola
Phaseolotoxin
Pht cluster
phtABC genes
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spelling Temperature-mediated biosynthesis of the phytotoxin phaseolotoxin by Pseudomonas syringae pv. phaseolicola depends on the autoregulated expression of the phtABC genesAguilera, SeleneÁlvarez Morales, ArielMurillo Martínez, JesúsHernández Flores, José LuisBravo, JaimeTorre Zavala, Susana de laPseudomonas syringae pv. PhaseolicolaPhaseolotoxinPht clusterphtABC genesPseudomonas syringae pv. phaseolicola produces phaseolotoxin in a temperature dependent manner, being optimally synthesized between 18ºC and 20ºC, while no detectable amounts are present above 28ºC. The Pht cluster, involved in the biosynthesis of phaseolotoxin, contains 23 genes that are organized in five transcriptional units. The function of most of the genes from the Pht cluster is still unknown and little information about the regulatory circuitry leading to expression of these genes has been reported. The purpose of the present study was to investigate the participation of pht genes in the regulation of the operons coded into the Pht cluster. We conducted Northern blot, uidA fusions and reverse transcription- PCR assays of pht genes in several mutants unable to produce phaseolotoxin. This allowed us to determine that, in P. syringae pv. phaseolicola NPS3121, genes phtABC are essential to prevent their own expression at 28ºC, a temperature at which no detectable amounts of the toxin are present. We obtained evidence that the phtABC genes also participate in the regulation of the phtD, phtM and phtL operons. According to our results, we propose that PhtABC and other Pht product activities could be involved in the synthesis of the sulfodiaminophosphinyl moiety of phaseolotoxin, which indirectly could be involved in the transcriptional regulation of the phtA operon.This work was funded by grants from the Consejo Nacional de Ciencia y Tecnología (CONACyT), research grant CB-2015-01-255155 to SA, and from the Spanish Plan Nacional I + D + i grant AGL2014-53242-C2-2-R, from the Ministerio de Economía y Competitividad, co-financed by the Fondo Europeo de Desarrollo Regional (FEDER) to JM.PLOSProducción AgrariaNekazaritza Ekoizpena2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2454/30538reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglésinfo:eu-repo/grantAgreement/MINECO//AGL2014-53242-C2-2-R© 2017 Aguilera et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/305382026-06-17T12:41:47Z
dc.title.none.fl_str_mv Temperature-mediated biosynthesis of the phytotoxin phaseolotoxin by Pseudomonas syringae pv. phaseolicola depends on the autoregulated expression of the phtABC genes
title Temperature-mediated biosynthesis of the phytotoxin phaseolotoxin by Pseudomonas syringae pv. phaseolicola depends on the autoregulated expression of the phtABC genes
spellingShingle Temperature-mediated biosynthesis of the phytotoxin phaseolotoxin by Pseudomonas syringae pv. phaseolicola depends on the autoregulated expression of the phtABC genes
Aguilera, Selene
Pseudomonas syringae pv. Phaseolicola
Phaseolotoxin
Pht cluster
phtABC genes
title_short Temperature-mediated biosynthesis of the phytotoxin phaseolotoxin by Pseudomonas syringae pv. phaseolicola depends on the autoregulated expression of the phtABC genes
title_full Temperature-mediated biosynthesis of the phytotoxin phaseolotoxin by Pseudomonas syringae pv. phaseolicola depends on the autoregulated expression of the phtABC genes
title_fullStr Temperature-mediated biosynthesis of the phytotoxin phaseolotoxin by Pseudomonas syringae pv. phaseolicola depends on the autoregulated expression of the phtABC genes
title_full_unstemmed Temperature-mediated biosynthesis of the phytotoxin phaseolotoxin by Pseudomonas syringae pv. phaseolicola depends on the autoregulated expression of the phtABC genes
title_sort Temperature-mediated biosynthesis of the phytotoxin phaseolotoxin by Pseudomonas syringae pv. phaseolicola depends on the autoregulated expression of the phtABC genes
dc.creator.none.fl_str_mv Aguilera, Selene
Álvarez Morales, Ariel
Murillo Martínez, Jesús
Hernández Flores, José Luis
Bravo, Jaime
Torre Zavala, Susana de la
author Aguilera, Selene
author_facet Aguilera, Selene
Álvarez Morales, Ariel
Murillo Martínez, Jesús
Hernández Flores, José Luis
Bravo, Jaime
Torre Zavala, Susana de la
author_role author
author2 Álvarez Morales, Ariel
Murillo Martínez, Jesús
Hernández Flores, José Luis
Bravo, Jaime
Torre Zavala, Susana de la
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Producción Agraria
Nekazaritza Ekoizpena
dc.subject.none.fl_str_mv Pseudomonas syringae pv. Phaseolicola
Phaseolotoxin
Pht cluster
phtABC genes
topic Pseudomonas syringae pv. Phaseolicola
Phaseolotoxin
Pht cluster
phtABC genes
description Pseudomonas syringae pv. phaseolicola produces phaseolotoxin in a temperature dependent manner, being optimally synthesized between 18ºC and 20ºC, while no detectable amounts are present above 28ºC. The Pht cluster, involved in the biosynthesis of phaseolotoxin, contains 23 genes that are organized in five transcriptional units. The function of most of the genes from the Pht cluster is still unknown and little information about the regulatory circuitry leading to expression of these genes has been reported. The purpose of the present study was to investigate the participation of pht genes in the regulation of the operons coded into the Pht cluster. We conducted Northern blot, uidA fusions and reverse transcription- PCR assays of pht genes in several mutants unable to produce phaseolotoxin. This allowed us to determine that, in P. syringae pv. phaseolicola NPS3121, genes phtABC are essential to prevent their own expression at 28ºC, a temperature at which no detectable amounts of the toxin are present. We obtained evidence that the phtABC genes also participate in the regulation of the phtD, phtM and phtL operons. According to our results, we propose that PhtABC and other Pht product activities could be involved in the synthesis of the sulfodiaminophosphinyl moiety of phaseolotoxin, which indirectly could be involved in the transcriptional regulation of the phtA operon.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/30538
url https://hdl.handle.net/2454/30538
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO//AGL2014-53242-C2-2-R
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PLOS
publisher.none.fl_str_mv PLOS
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
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repository.mail.fl_str_mv
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