A cryopreservation method for bioengineered 3D cell culture models

Technologies to cryogenically preserve (a.k.a. cryopreserve) living tissue, cell lines and primary cells have matured greatly for both clinicians and researchers since their first demonstration in the 1950s and are widely used in storage and transport applications. Currently, however, there remains...

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Detalhes bibliográficos
Autores: Herrero Gómez, Alba, Azagra, Marc, Marco Rius, Irene
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/188921
Acesso em linha:https://hdl.handle.net/2445/188921
Access Level:acceso abierto
Palavra-chave:Criobiologia
Bioenginyeria
Cultiu cel·lular
Cryobiology
Bioengineering
Cell culture
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spelling A cryopreservation method for bioengineered 3D cell culture modelsHerrero Gómez, AlbaAzagra, MarcMarco Rius, IreneCriobiologiaBioenginyeriaCultiu cel·lularCryobiologyBioengineeringCell cultureTechnologies to cryogenically preserve (a.k.a. cryopreserve) living tissue, cell lines and primary cells have matured greatly for both clinicians and researchers since their first demonstration in the 1950s and are widely used in storage and transport applications. Currently, however, there remains an absence of viable cryopreservation and thawing methods for bioengineered, three-dimensional (3D) cell models, including patients' samples. As a first step towards addressing this gap, we demonstrate a viable protocol for spheroid cryopreservation and survival based on a 3D carboxymethyl cellulose scaffold and precise conditions for freezing and thawing. The protocol is tested using hepatocytes, for which the scaffold provides both the 3D structure for cells to self-arrange into spheroids and to support cells during freezing for optimal post-thaw viability. Cell viability after thawing is improved compared to conventional pellet models where cells settle under gravity to form a pseudo-tissue before freezing. The technique may advance cryobiology and other applications that demand high-integrity transport of pre-assembled 3D models (from cell lines and in future cells from patients) between facilities, for example between medical practice, research and testing facilities.2022202220222022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion9 p.application/pdfhttps://hdl.handle.net/2445/188921Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1088/1748-605X/ac76fbBiomedical Materials, 2022, vol. 17, num. 4, p. 045023https://doi.org/10.1088/1748-605X/ac76fbinfo:eu-repo/grantAgreement/EC/H2020/863037cc by (c) Herrero Gomez, Alba et al., 2022http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1889212026-05-29T05:05:01Z
dc.title.none.fl_str_mv A cryopreservation method for bioengineered 3D cell culture models
title A cryopreservation method for bioengineered 3D cell culture models
spellingShingle A cryopreservation method for bioengineered 3D cell culture models
Herrero Gómez, Alba
Criobiologia
Bioenginyeria
Cultiu cel·lular
Cryobiology
Bioengineering
Cell culture
title_short A cryopreservation method for bioengineered 3D cell culture models
title_full A cryopreservation method for bioengineered 3D cell culture models
title_fullStr A cryopreservation method for bioengineered 3D cell culture models
title_full_unstemmed A cryopreservation method for bioengineered 3D cell culture models
title_sort A cryopreservation method for bioengineered 3D cell culture models
dc.creator.none.fl_str_mv Herrero Gómez, Alba
Azagra, Marc
Marco Rius, Irene
author Herrero Gómez, Alba
author_facet Herrero Gómez, Alba
Azagra, Marc
Marco Rius, Irene
author_role author
author2 Azagra, Marc
Marco Rius, Irene
author2_role author
author
dc.subject.none.fl_str_mv Criobiologia
Bioenginyeria
Cultiu cel·lular
Cryobiology
Bioengineering
Cell culture
topic Criobiologia
Bioenginyeria
Cultiu cel·lular
Cryobiology
Bioengineering
Cell culture
description Technologies to cryogenically preserve (a.k.a. cryopreserve) living tissue, cell lines and primary cells have matured greatly for both clinicians and researchers since their first demonstration in the 1950s and are widely used in storage and transport applications. Currently, however, there remains an absence of viable cryopreservation and thawing methods for bioengineered, three-dimensional (3D) cell models, including patients' samples. As a first step towards addressing this gap, we demonstrate a viable protocol for spheroid cryopreservation and survival based on a 3D carboxymethyl cellulose scaffold and precise conditions for freezing and thawing. The protocol is tested using hepatocytes, for which the scaffold provides both the 3D structure for cells to self-arrange into spheroids and to support cells during freezing for optimal post-thaw viability. Cell viability after thawing is improved compared to conventional pellet models where cells settle under gravity to form a pseudo-tissue before freezing. The technique may advance cryobiology and other applications that demand high-integrity transport of pre-assembled 3D models (from cell lines and in future cells from patients) between facilities, for example between medical practice, research and testing facilities.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/188921
url https://hdl.handle.net/2445/188921
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1088/1748-605X/ac76fb
Biomedical Materials, 2022, vol. 17, num. 4, p. 045023
https://doi.org/10.1088/1748-605X/ac76fb
info:eu-repo/grantAgreement/EC/H2020/863037
dc.rights.none.fl_str_mv cc by (c) Herrero Gomez, Alba et al., 2022
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Herrero Gomez, Alba et al., 2022
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9 p.
application/pdf
dc.source.none.fl_str_mv Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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