From vial to injector: Stability, degradation, and surface interactions of non-derivatized ecgonine methyl ester in GC–MS

Ecgonine methyl ester (EME) is a primary metabolite of cocaine widely used in forensic toxicology, yet it is often regarded as problematic for direct GC–MS analysis due to presumed thermal instability and surface-related losses. In this study, the stability of EME and its isotopically labeled analog...

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Bibliographic Details
Authors: Rubio, Nélida Cristina, Álvarez Freire, Iván, Cabarcos Fernández, Pamela, Tabernero Duque, María Jesús, Sánchez Sellero, Inés, Bermejo Barrera, Ana María
Format: article
Publication Date:2026
Country:España
Institution:Universidad de Santiago de Compostela (USC)
Repository:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Language:English
OAI Identifier:oai:dnet:minerva_____::ed899818fca69570d473f1f62b66eec1
Online Access:https://hdl.handle.net/10347/47588
Access Level:Open access
Keyword:Ecgonine methy ester
Anhydroecgonine methyl ester
Stability
GC–MS
3214 Toxicología
3203 Medicina forense
Description
Summary:Ecgonine methyl ester (EME) is a primary metabolite of cocaine widely used in forensic toxicology, yet it is often regarded as problematic for direct GC–MS analysis due to presumed thermal instability and surface-related losses. In this study, the stability of EME and its isotopically labeled analogue (EME-d₃) was systematically investigated under realistic laboratory conditions relevant to forensic workflows. Injector temperature, solvent composition, storage conditions, container material, evaporation steps, and matrix effects were evaluated using GC–MS. The results demonstrate that EME does not undergo significant thermal degradation during GC–MS injection, even at elevated injector temperatures, and that in-injector formation of anhydroecgonine methyl ester (AEME) is negligible. Instead, AEME formation arises predominantly from slow, solution-phase dehydration prior to injection, strongly influenced by solvent type, pH, vial material, and matrix composition. Methanol promoted degradation, whereas acetonitrile and mild acidification markedly improved stability. Biological matrices, particularly urine extracts, exerted a protective effect, suppressing dehydration during autosampler storage. A pronounced positive matrix effect was observed for EME in GC–MS, particularly in urine extracts, and was only partially compensated by the isotopically labeled internal standard. Adsorptive losses during evaporation were a major preanalytical issue: nearly quantitative in polypropylene and only partially mitigated by a keeper in glass vials. Overall, these findings show that the analytical challenges associated with EME are primarily preanalytical rather than instrumental. When appropriate handling strategies are applied, EME and EME-d₃ can be reliably quantified by GC–MS without derivatization, supporting their broader use in forensic toxicology.