Spanish Multicenter Study of the Epidemiology and Mechanisms of Amoxicillin-Clavulanate Resistance in Escherichia coli

We conducted a prospective multicenter study in Spain to characterize the mechanisms of resistance to amoxicillin-clavu-lanate (AMC) in Escherichia coli. Up to 44 AMC-resistant E. coli isolates (MIC>32/16 g/ml) were collected at each of theseven participant hospitals. Resistance mechanisms were c...

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Autores: Ortega, Adriana, Oteo, Jesús, Aranzamendi-Zaldumbide, Maitane, Bartolomé, Rosa M., Bou, Germán, Cercenado, Emilia, Conejo Gonzalo, Mª Carmen, González-López, Juan José, Marín, Mercedes, Martínez-Martínez, Luis, Marino, María, Ferran, Navarro, Oliver, Antonio, Pascual Hernández, Álvaro, Rivera, Alba, Rodríguez-Baño, Jesús, Weber, Irene, Aracil, Belén, Campos, José
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/40585
Acceso en línea:http://hdl.handle.net/11441/40585
https://doi.org/10.1128/AAC.06393-11
Access Level:acceso abierto
Palabra clave:Combinación de amoxicilina clavulanato potásico
Farmacorresistencia bacteriana
Infecciones por Escherichia coli
Epidemiología molecular
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spelling Spanish Multicenter Study of the Epidemiology and Mechanisms of Amoxicillin-Clavulanate Resistance in Escherichia coliOrtega, AdrianaOteo, JesúsAranzamendi-Zaldumbide, MaitaneBartolomé, Rosa M.Bou, GermánCercenado, EmiliaConejo Gonzalo, Mª CarmenGonzález-López, Juan JoséMarín, MercedesMartínez-Martínez, LuisMarino, MaríaFerran, NavarroOliver, AntonioPascual Hernández, ÁlvaroRivera, AlbaRodríguez-Baño, JesúsWeber, IreneAracil, BelénCampos, JoséCombinación de amoxicilina clavulanato potásicoFarmacorresistencia bacterianaInfecciones por Escherichia coliEpidemiología molecularWe conducted a prospective multicenter study in Spain to characterize the mechanisms of resistance to amoxicillin-clavu-lanate (AMC) in Escherichia coli. Up to 44 AMC-resistant E. coli isolates (MIC>32/16 g/ml) were collected at each of theseven participant hospitals. Resistance mechanisms were characterized by PCR and sequencing. Molecular epidemiology was studied by pulsed-field gel electrophoresis (PFGE) and by multilocus sequence typing. Overall AMC resistance was 9.3%. The resistance mechanisms detected in the 257 AMC-resistant isolates were OXA-1 production (26.1%), hyperpro-duction of penicillinase (22.6%), production of plasmidic AmpC (19.5%), hyperproduction of chromosomic AmpC(18.3%), and production of inhibitor-resistant TEM (IRT) (17.5%). The IRTs identified were TEM-40 (33.3%), TEM-30(28.9%), TEM-33 (11.1%), TEM-32 (4.4%), TEM-34 (4.4%), TEM-35 (2.2%), TEM-54 (2.2%), TEM-76 (2.2%), TEM-79(2.2%), and the new TEM-185 (8.8%). By PFGE, a high degree of genetic diversity was observed although two well-defined clusters were detected in the OXA-1-producing isolates: the C1 cluster consisting of 19 phylogroup A/sequence type 88[ST88] isolates and the C2 cluster consisting of 19 phylogroup B2/ST131 isolates (16 of them producing CTX-M-15). Each of the clusters was detected in six different hospitals. In total, 21.8% of the isolates were serotype O25b/phylogroup B2 (O25b/B2). AMC resistance in E. coli is widespread in Spain at the hospital and community levels. A high prevalence of OXA-1 was found. Although resistant isolates were genetically diverse, clonality was linked to OXA-1-producing isolates of the STs 88 and 131. Dissemination of IRTs was frequent, and the epidemic O25b/B2/ST131 clone carried many different mechanisms of AMC resistance.American Society for MicrobiologyMicrobiologíaMedicina2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/40585https://doi.org/10.1128/AAC.06393-11reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésAntimicrobial Agents and Chemotherapy, 56(7), 3576-3581http://aac.asm.org/content/56/7/3576.abstractinfo:eu-repo/semantics/openAccessoai:idus.us.es:11441/405852026-06-17T12:51:07Z
dc.title.none.fl_str_mv Spanish Multicenter Study of the Epidemiology and Mechanisms of Amoxicillin-Clavulanate Resistance in Escherichia coli
title Spanish Multicenter Study of the Epidemiology and Mechanisms of Amoxicillin-Clavulanate Resistance in Escherichia coli
spellingShingle Spanish Multicenter Study of the Epidemiology and Mechanisms of Amoxicillin-Clavulanate Resistance in Escherichia coli
Ortega, Adriana
Combinación de amoxicilina clavulanato potásico
Farmacorresistencia bacteriana
Infecciones por Escherichia coli
Epidemiología molecular
title_short Spanish Multicenter Study of the Epidemiology and Mechanisms of Amoxicillin-Clavulanate Resistance in Escherichia coli
title_full Spanish Multicenter Study of the Epidemiology and Mechanisms of Amoxicillin-Clavulanate Resistance in Escherichia coli
title_fullStr Spanish Multicenter Study of the Epidemiology and Mechanisms of Amoxicillin-Clavulanate Resistance in Escherichia coli
title_full_unstemmed Spanish Multicenter Study of the Epidemiology and Mechanisms of Amoxicillin-Clavulanate Resistance in Escherichia coli
title_sort Spanish Multicenter Study of the Epidemiology and Mechanisms of Amoxicillin-Clavulanate Resistance in Escherichia coli
dc.creator.none.fl_str_mv Ortega, Adriana
Oteo, Jesús
Aranzamendi-Zaldumbide, Maitane
Bartolomé, Rosa M.
Bou, Germán
Cercenado, Emilia
Conejo Gonzalo, Mª Carmen
González-López, Juan José
Marín, Mercedes
Martínez-Martínez, Luis
Marino, María
Ferran, Navarro
Oliver, Antonio
Pascual Hernández, Álvaro
Rivera, Alba
Rodríguez-Baño, Jesús
Weber, Irene
Aracil, Belén
Campos, José
author Ortega, Adriana
author_facet Ortega, Adriana
Oteo, Jesús
Aranzamendi-Zaldumbide, Maitane
Bartolomé, Rosa M.
Bou, Germán
Cercenado, Emilia
Conejo Gonzalo, Mª Carmen
González-López, Juan José
Marín, Mercedes
Martínez-Martínez, Luis
Marino, María
Ferran, Navarro
Oliver, Antonio
Pascual Hernández, Álvaro
Rivera, Alba
Rodríguez-Baño, Jesús
Weber, Irene
Aracil, Belén
Campos, José
author_role author
author2 Oteo, Jesús
Aranzamendi-Zaldumbide, Maitane
Bartolomé, Rosa M.
Bou, Germán
Cercenado, Emilia
Conejo Gonzalo, Mª Carmen
González-López, Juan José
Marín, Mercedes
Martínez-Martínez, Luis
Marino, María
Ferran, Navarro
Oliver, Antonio
Pascual Hernández, Álvaro
Rivera, Alba
Rodríguez-Baño, Jesús
Weber, Irene
Aracil, Belén
Campos, José
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Microbiología
Medicina
dc.subject.none.fl_str_mv Combinación de amoxicilina clavulanato potásico
Farmacorresistencia bacteriana
Infecciones por Escherichia coli
Epidemiología molecular
topic Combinación de amoxicilina clavulanato potásico
Farmacorresistencia bacteriana
Infecciones por Escherichia coli
Epidemiología molecular
description We conducted a prospective multicenter study in Spain to characterize the mechanisms of resistance to amoxicillin-clavu-lanate (AMC) in Escherichia coli. Up to 44 AMC-resistant E. coli isolates (MIC>32/16 g/ml) were collected at each of theseven participant hospitals. Resistance mechanisms were characterized by PCR and sequencing. Molecular epidemiology was studied by pulsed-field gel electrophoresis (PFGE) and by multilocus sequence typing. Overall AMC resistance was 9.3%. The resistance mechanisms detected in the 257 AMC-resistant isolates were OXA-1 production (26.1%), hyperpro-duction of penicillinase (22.6%), production of plasmidic AmpC (19.5%), hyperproduction of chromosomic AmpC(18.3%), and production of inhibitor-resistant TEM (IRT) (17.5%). The IRTs identified were TEM-40 (33.3%), TEM-30(28.9%), TEM-33 (11.1%), TEM-32 (4.4%), TEM-34 (4.4%), TEM-35 (2.2%), TEM-54 (2.2%), TEM-76 (2.2%), TEM-79(2.2%), and the new TEM-185 (8.8%). By PFGE, a high degree of genetic diversity was observed although two well-defined clusters were detected in the OXA-1-producing isolates: the C1 cluster consisting of 19 phylogroup A/sequence type 88[ST88] isolates and the C2 cluster consisting of 19 phylogroup B2/ST131 isolates (16 of them producing CTX-M-15). Each of the clusters was detected in six different hospitals. In total, 21.8% of the isolates were serotype O25b/phylogroup B2 (O25b/B2). AMC resistance in E. coli is widespread in Spain at the hospital and community levels. A high prevalence of OXA-1 was found. Although resistant isolates were genetically diverse, clonality was linked to OXA-1-producing isolates of the STs 88 and 131. Dissemination of IRTs was frequent, and the epidemic O25b/B2/ST131 clone carried many different mechanisms of AMC resistance.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/40585
https://doi.org/10.1128/AAC.06393-11
url http://hdl.handle.net/11441/40585
https://doi.org/10.1128/AAC.06393-11
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Antimicrobial Agents and Chemotherapy, 56(7), 3576-3581
http://aac.asm.org/content/56/7/3576.abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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