Lectin-binding pattern of Senegalese sole Solea senegalensis (Kaup) testis

20 p., tablas, figuras y bibliografía

Detalles Bibliográficos
Autores: Desantis, Salvatore, Zizza, Sara, García-López, Ángel, Sciscioli, V., Mañanós, Evaristo L., De Metrio, Gragorio, Sarasquete, Carmen
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/45720
Acceso en línea:http://hdl.handle.net/10261/45720
Access Level:acceso abierto
Palabra clave:Testis
Spermatogenesis
Glycoconjugates
Lectins
Teleost
Pleuronectiformes
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spelling Lectin-binding pattern of Senegalese sole Solea senegalensis (Kaup) testisGlycoconjugates in the Senegalese sole testisDesantis, SalvatoreZizza, SaraGarcía-López, ÁngelSciscioli, V.Mañanós, Evaristo L.De Metrio, GragorioSarasquete, CarmenTestisSpermatogenesisGlycoconjugatesLectinsTeleostPleuronectiformes20 p., tablas, figuras y bibliografíaThe localization and characterization of oligosaccharide sequences in the testis of Senegalese sole Solea senegalensis was investigated using 12 lectins in combination with KOH saponification and sialidase digestion (K-s). The interstitial compartment contained all the sugar residues investigated, those bearing oligosaccharides terminating with sialic acid (Neu5Ac)alpha2,3Galbeta1,4GlcNAc, Neu5AcGalNAcalpha1,3(LFucbeta1,2)Galbeta1,3/4GlcNAcbeta1 and GalNAcalpha1,3(LFuc1,2) Galbeta1,3/4GlcNAcbeta1 being more abundant in the medullar region than in the cortex. The melano-macrophage centres found in the interstitial compartment displayed glycans terminating with Galbeta1,3GalNAc. The basal lamina separating the germinal and interstitial compartments exhibited glycans with terminal/internal mannose, internal betaGlcNAc, and terminal Neu5Acalpha2,6Gal/GalNAc, and Neu5AcGalbeta1,3GalNAc, Galbeta1,3GalNAc (PNA), Galbeta1,4GlcNAc, GalNAc, alphaGal, and alphaL-Fuc. In the germinal compartment, the Sertoli cells expressed only glycans terminating with Neu5Acalpha2,3Galbeta1,4GlcNAc in the apical and supra-nuclear lateral surface of the spermatonial cysts located in the distal part of the seminiferous lobules. Primary spermatocytes exhibited oligosaccharides terminating with Galbeta1,3GalNAc and alphaGalNAc in the cytoplasm and nucleus, respectively. The spermatids contained highly mannosylated glycans terminating with GalNac, alphaGal, and alphaL-Fuc. The head of spermatozoa expressed a more complex glycosylation pattern characterized by the additional presence of oligosaccharides terminating with Neu5Acalpha2,3Galbeta1,4GlcNAc, Neu5AcGalbeta1,3GalNAc, Neu5AcGalNAcalpha1,3(LFuca1,2)Galbeta1,3/4GlcNAcbeta1, GalNAcalpha1,3(LFucalpha1,2 Galbeta1,3/4GlcNAcbeta1. The comparison with previous lectin histochemical studies carried out in other fish species reveals a specific glycosylation pattern of Senegalese sole testicular structures and spermatozoa head.This research was funded by the Spanish Ministry of Education and Science-MEC-(Project AGL2006-13777)Universidad de Murcia201220122010info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/45720reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/457202026-05-22T06:33:51Z
dc.title.none.fl_str_mv Lectin-binding pattern of Senegalese sole Solea senegalensis (Kaup) testis
Glycoconjugates in the Senegalese sole testis
title Lectin-binding pattern of Senegalese sole Solea senegalensis (Kaup) testis
spellingShingle Lectin-binding pattern of Senegalese sole Solea senegalensis (Kaup) testis
Desantis, Salvatore
Testis
Spermatogenesis
Glycoconjugates
Lectins
Teleost
Pleuronectiformes
title_short Lectin-binding pattern of Senegalese sole Solea senegalensis (Kaup) testis
title_full Lectin-binding pattern of Senegalese sole Solea senegalensis (Kaup) testis
title_fullStr Lectin-binding pattern of Senegalese sole Solea senegalensis (Kaup) testis
title_full_unstemmed Lectin-binding pattern of Senegalese sole Solea senegalensis (Kaup) testis
title_sort Lectin-binding pattern of Senegalese sole Solea senegalensis (Kaup) testis
dc.creator.none.fl_str_mv Desantis, Salvatore
Zizza, Sara
García-López, Ángel
Sciscioli, V.
Mañanós, Evaristo L.
De Metrio, Gragorio
Sarasquete, Carmen
author Desantis, Salvatore
author_facet Desantis, Salvatore
Zizza, Sara
García-López, Ángel
Sciscioli, V.
Mañanós, Evaristo L.
De Metrio, Gragorio
Sarasquete, Carmen
author_role author
author2 Zizza, Sara
García-López, Ángel
Sciscioli, V.
Mañanós, Evaristo L.
De Metrio, Gragorio
Sarasquete, Carmen
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Testis
Spermatogenesis
Glycoconjugates
Lectins
Teleost
Pleuronectiformes
topic Testis
Spermatogenesis
Glycoconjugates
Lectins
Teleost
Pleuronectiformes
description 20 p., tablas, figuras y bibliografía
publishDate 2010
dc.date.none.fl_str_mv 2010
2012
2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/45720
url http://hdl.handle.net/10261/45720
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidad de Murcia
publisher.none.fl_str_mv Universidad de Murcia
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869417794352709632
score 15,811543

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