Supplemental data: Arabidopsis FIBRILLIN6 influences carotenoid biosynthesis by directly promoting phytoene synthase activity

Supplemental Figure S1. Characterization of 35S:FBN6-RFP lines.-- Supplemental Table S1. Primers used in this work.-- Supplemental Table S2. Phytoene levels in control and NFZ-treated N. benthamiana leaves agroinfiltrated with FBN6 and/or PSY constructs.-- Supplemental Table S3. Photosynthetic pigme...

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Detalhes bibliográficos
Autores: Iglesias-Sanchez, Ariadna, Navarro-Carcelen, Juan, Morelli, Luca, Rodriguez-Concepcion, Manuel
Formato: conjunto de datos
Fecha de publicación:2024
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/351834
Acesso em linha:http://hdl.handle.net/10261/351834
Access Level:acceso abierto
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dc.title.none.fl_str_mv Supplemental data: Arabidopsis FIBRILLIN6 influences carotenoid biosynthesis by directly promoting phytoene synthase activity
title Supplemental data: Arabidopsis FIBRILLIN6 influences carotenoid biosynthesis by directly promoting phytoene synthase activity
spellingShingle Supplemental data: Arabidopsis FIBRILLIN6 influences carotenoid biosynthesis by directly promoting phytoene synthase activity
Iglesias-Sanchez, Ariadna
title_short Supplemental data: Arabidopsis FIBRILLIN6 influences carotenoid biosynthesis by directly promoting phytoene synthase activity
title_full Supplemental data: Arabidopsis FIBRILLIN6 influences carotenoid biosynthesis by directly promoting phytoene synthase activity
title_fullStr Supplemental data: Arabidopsis FIBRILLIN6 influences carotenoid biosynthesis by directly promoting phytoene synthase activity
title_full_unstemmed Supplemental data: Arabidopsis FIBRILLIN6 influences carotenoid biosynthesis by directly promoting phytoene synthase activity
title_sort Supplemental data: Arabidopsis FIBRILLIN6 influences carotenoid biosynthesis by directly promoting phytoene synthase activity
dc.creator.none.fl_str_mv Iglesias-Sanchez, Ariadna
Navarro-Carcelen, Juan
Morelli, Luca
Rodriguez-Concepcion, Manuel
author Iglesias-Sanchez, Ariadna
author_facet Iglesias-Sanchez, Ariadna
Navarro-Carcelen, Juan
Morelli, Luca
Rodriguez-Concepcion, Manuel
author_role author
author2 Navarro-Carcelen, Juan
Morelli, Luca
Rodriguez-Concepcion, Manuel
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
European Commission
Ministerio de Ciencia e Innovación (España)
Generalitat Valenciana
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description Supplemental Figure S1. Characterization of 35S:FBN6-RFP lines.-- Supplemental Table S1. Primers used in this work.-- Supplemental Table S2. Phytoene levels in control and NFZ-treated N. benthamiana leaves agroinfiltrated with FBN6 and/or PSY constructs.-- Supplemental Table S3. Photosynthetic pigment levels in N. benthamiana leaves agroinfiltrated with FBN6, PSY, and/or crtB constructs.-- Supplemental Table S4. Photosynthetic pigment levels in etiolated and de-etiolating WT and fbn6 Arabidopsis seedlings.-- Supplemental Table S5. Photosynthetic pigment levels in WT and fbn6 Arabidopsis seedlings exposed to HL.
publishDate 2024
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Iglesias-Sanchez, Ariadna; Navarro-Carcelen, Juan; Morelli, Luca; Rodriguez-Concepcion, Manuel. Arabidopsis FIBRILLIN6 influences carotenoid biosynthesis by directly promoting phytoene synthase activity. https://doi.org/10.1093/plphys/kiad613. http://hdl.handle.net/10261/351828
https://doi.org/10.1093/plphys/kiad613
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dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
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spelling Supplemental data: Arabidopsis FIBRILLIN6 influences carotenoid biosynthesis by directly promoting phytoene synthase activityIglesias-Sanchez, AriadnaNavarro-Carcelen, JuanMorelli, LucaRodriguez-Concepcion, ManuelSupplemental Figure S1. Characterization of 35S:FBN6-RFP lines.-- Supplemental Table S1. Primers used in this work.-- Supplemental Table S2. Phytoene levels in control and NFZ-treated N. benthamiana leaves agroinfiltrated with FBN6 and/or PSY constructs.-- Supplemental Table S3. Photosynthetic pigment levels in N. benthamiana leaves agroinfiltrated with FBN6, PSY, and/or crtB constructs.-- Supplemental Table S4. Photosynthetic pigment levels in etiolated and de-etiolating WT and fbn6 Arabidopsis seedlings.-- Supplemental Table S5. Photosynthetic pigment levels in WT and fbn6 Arabidopsis seedlings exposed to HL.Carotenoids are health-promoting plastidial isoprenoids with essential functions in plants as photoprotectants and photosynthetic pigments in chloroplasts. They also accumulate in specialized plastids named chromoplasts, providing color to non-photosynthetic tissues such as flower petals and ripe fruit. Carotenoid accumulation in chromoplasts requires specialized structures and proteins such as fibrillins (FBNs). The FBN family includes structural components of carotenoid sequestering structures in chromoplasts and members with metabolic roles in chloroplasts and other plastid types. However, the association of FBNs with carotenoids in plastids other than chromoplasts has remained unexplored. Here, we show that Arabidopsis (Arabidopsis thaliana) FBN6 interacts with phytoene synthase (PSY), the first enzyme of the carotenoid pathway. FBN6, but not FBN4 (a FBN that does not interact with PSY), enhances the activity of plant PSY (but not of the bacterial PSY crtB) in Escherichia coli cells. Overexpression of FBN6 in Nicotiana benthamiana leaves results in a higher production of phytoene, the product of PSY activity, whereas loss of FBN6 activity in Arabidopsis mutants dramatically reduces the production of carotenoids during seedling de-etiolation and after exposure to high light. Our work hence demonstrates that FBNs promote not only the accumulation of carotenoids in chromoplasts but also their biosynthesis in chloroplasts.This work was funded by grants from Spanish MCIN/AEI/10.13039/501100011033 and European NextGeneration EU/PRTR and PRIMA programs to M.R.-C. (PID2020-115810GB-I00 and UToPIQ-PCI2021-121941). M.R.-C. is also supported by Generalitat Valenciana (PROMETEU/2021/056 and AGROALNEXT/2022/067). Our group is a member of CaRed (Spanish Carotenoid Network) funded by MCIN/AEI (RED2022-134577-T). A.I.-S. and J.N.-C. received predoctoral fellowships from MCIN/AEI (PRE2018-083610 and PRE2021-098681, respectively).Peer reviewedOxford University PressMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European CommissionMinisterio de Ciencia e Innovación (España)Generalitat ValencianaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1application/pdfhttp://hdl.handle.net/10261/351834reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115810GB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PCI2021-121941info:eu-repo/grantAgreement/AEI//RED2022-134577-Tinfo:eu-repo/grantAgreement/AEI//PRE2018-083610info:eu-repo/grantAgreement/AEI//PRE2021-098681Iglesias-Sanchez, Ariadna; Navarro-Carcelen, Juan; Morelli, Luca; Rodriguez-Concepcion, Manuel. Arabidopsis FIBRILLIN6 influences carotenoid biosynthesis by directly promoting phytoene synthase activity. https://doi.org/10.1093/plphys/kiad613. http://hdl.handle.net/10261/351828https://doi.org/10.1093/plphys/kiad613Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3518342026-05-22T06:33:51Z
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