Complex transcriptional regulation and independent evolution of fungal-like traits in a relative of animals

Cell-type specification through differential genome regulation is a hallmark of complex multicellularity. However, it remains unclear how this process evolved during the transition from unicellular to multicellular organisms. To address this question, we investigated transcriptional dynamics in the...

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Detalles Bibliográficos
Autores: de Mendoza, Alex, Suga, Hiroshi, Permanyer, Jon, Irimia Martínez, Manuel, Ruíz Trillo, Iñaki
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/26877
Acceso en línea:http://hdl.handle.net/10230/26877
http://dx.doi.org/10.1016/10.7554/eLife.08904
Access Level:acceso abierto
Palabra clave:Biologia del desenvolupament
Cèl·lules mare
Alternative splicing
Comparative genomics
Developmental biology
Evolutionary biology
Genomics
Lateral gene transfer
lincRNA
Multicellularity
Secretome
Stem cells
Descripción
Sumario:Cell-type specification through differential genome regulation is a hallmark of complex multicellularity. However, it remains unclear how this process evolved during the transition from unicellular to multicellular organisms. To address this question, we investigated transcriptional dynamics in the ichthyosporean Creolimax fragrantissima, a relative of animals that undergoes coenocytic development. We find that Creolimax utilizes dynamic regulation of alternative splicing, long inter-genic non-coding RNAs and co-regulated gene modules associated with animal multicellularity in a cell-type specific manner. Moreover, our study suggests that the different cell types of the three closest animal relatives (ichthyosporeans, filastereans and choanoflagellates) are the product of lineage-specific innovations. Additionally, a proteomic survey of the secretome reveals adaptations to a fungal-like lifestyle. In summary, the diversity of cell types among protistan relatives of animals and their complex genome regulation demonstrates that the last unicellular ancestor of animals was already capable of elaborate specification of cell types.