Tandem Mass Tagging (TMT) Reveals Tissue-Specific Proteome of L4 Larvae of Anisakis simplex s. s.: Enzymes of Energy and/or Carbohydrate Metabolism as Potential Drug Targets in Anisakiasis

29 pages, 9 figures, 2 tables.-- This is an open access article distributed under the Creative Commons Attribution License

Detalhes bibliográficos
Autores: Stryiński, Robert, Mateos, Jesús, Carrera, Mónica, Jastrzebski, Jan Pawel, Bogacka, Iwona, Lopieńska-Biernat, Elzbieta
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/275043
Acesso em linha:http://hdl.handle.net/10261/275043
Access Level:acceso abierto
Palavra-chave:Anisakis simplex
L4 stage larvae
Intestine
Cuticle
Folliculin
Oxoglutarate dehydrogenase
Proteomics
LC-MS/MS
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spelling Tandem Mass Tagging (TMT) Reveals Tissue-Specific Proteome of L4 Larvae of Anisakis simplex s. s.: Enzymes of Energy and/or Carbohydrate Metabolism as Potential Drug Targets in AnisakiasisStryiński, RobertMateos, JesúsCarrera, MónicaJastrzebski, Jan PawelBogacka, IwonaLopieńska-Biernat, ElzbietaAnisakis simplexL4 stage larvaeIntestineCuticleFolliculinOxoglutarate dehydrogenaseProteomicsLC-MS/MS29 pages, 9 figures, 2 tables.-- This is an open access article distributed under the Creative Commons Attribution LicenseAnisakis simplex s. s. is a parasitic nematode of marine mammals and causative agent of anisakiasis in humans. The cuticle and intestine of the larvae are the tissues most responsible for direct and indirect contact, respectively, of the parasite with the host. At the L4 larval stage, tissues, such as the cuticle and intestine, are fully developed and functional, in contrast to the L3 stage. As such, this work provides for the first time the tissue-specific proteome of A. simplex s. s. larvae in the L4 stage. Statistical analysis (FC ≥ 2; p-value ≤ 0.01) showed that 107 proteins were differentially regulated (DRPs) between the cuticle and the rest of the larval body. In the comparison between the intestine and the rest of the larval body at the L4 stage, 123 proteins were identified as DRPs. Comparison of the individual tissues examined revealed a total of 272 DRPs, with 133 proteins more abundant in the cuticle and 139 proteins more abundant in the intestine. Detailed functional analysis of the identified proteins was performed using bioinformatics tools. Glycolysis and the tricarboxylic acid cycle were the most enriched metabolic pathways by cuticular and intestinal proteins, respectively, in the L4 stage of A. simplex s. s. The presence of two proteins, folliculin (FLCN) and oxoglutarate dehydrogenase (OGDH), was confirmed by Western blot, and their tertiary structure was predicted and compared with other species. In addition, host–pathogen interactions were identified, and potential new allergens were predicted. The result of this manuscript shows the largest number of protein identifications to our knowledge using proteomics tools for different tissues of L4 larvae of A. simplex s. s. The identified tissue-specific proteins could serve as targets for new drugs against anisakiasisThis work was supported by National Science Centre of Poland, grant no. 2018/31/B/NZ9/01683. Additionally, this work was supported by the “Development Program of the University of Warmia and Mazury in Olsztyn”, co-financed by the European Union under the European Social Fund from the Operational Program “Knowledge Education Development”. Robert Stryinski is a recipient of a scholarship from the program “Interdisciplinary Doctoral Studies in Biology and Biotechnology” (POWR.03.05.00-00-Z310/17), which is funded by the European Social Fund. The LC-MS/MS analysis part of this work was funded by the GAIN-Xunta de Galicia, project number IN607D 2017/01 and the Spanish AEI/EU-FEDER PID2019-103845RB-C21 projectPeer reviewedMultidisciplinary Digital Publishing InstituteNational Science Centre (Poland)European CommissionXunta de GaliciaMinisterio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/275043reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-103845RB-C21The underlying dataset has been published as supplementary material of the article in the publishers platform at DOI https://doi.org/10.3390/ijms23084336https://doi.org/10.3390/ijms23084336Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2750432026-05-22T06:33:51Z
dc.title.none.fl_str_mv Tandem Mass Tagging (TMT) Reveals Tissue-Specific Proteome of L4 Larvae of Anisakis simplex s. s.: Enzymes of Energy and/or Carbohydrate Metabolism as Potential Drug Targets in Anisakiasis
title Tandem Mass Tagging (TMT) Reveals Tissue-Specific Proteome of L4 Larvae of Anisakis simplex s. s.: Enzymes of Energy and/or Carbohydrate Metabolism as Potential Drug Targets in Anisakiasis
spellingShingle Tandem Mass Tagging (TMT) Reveals Tissue-Specific Proteome of L4 Larvae of Anisakis simplex s. s.: Enzymes of Energy and/or Carbohydrate Metabolism as Potential Drug Targets in Anisakiasis
Stryiński, Robert
Anisakis simplex
L4 stage larvae
Intestine
Cuticle
Folliculin
Oxoglutarate dehydrogenase
Proteomics
LC-MS/MS
title_short Tandem Mass Tagging (TMT) Reveals Tissue-Specific Proteome of L4 Larvae of Anisakis simplex s. s.: Enzymes of Energy and/or Carbohydrate Metabolism as Potential Drug Targets in Anisakiasis
title_full Tandem Mass Tagging (TMT) Reveals Tissue-Specific Proteome of L4 Larvae of Anisakis simplex s. s.: Enzymes of Energy and/or Carbohydrate Metabolism as Potential Drug Targets in Anisakiasis
title_fullStr Tandem Mass Tagging (TMT) Reveals Tissue-Specific Proteome of L4 Larvae of Anisakis simplex s. s.: Enzymes of Energy and/or Carbohydrate Metabolism as Potential Drug Targets in Anisakiasis
title_full_unstemmed Tandem Mass Tagging (TMT) Reveals Tissue-Specific Proteome of L4 Larvae of Anisakis simplex s. s.: Enzymes of Energy and/or Carbohydrate Metabolism as Potential Drug Targets in Anisakiasis
title_sort Tandem Mass Tagging (TMT) Reveals Tissue-Specific Proteome of L4 Larvae of Anisakis simplex s. s.: Enzymes of Energy and/or Carbohydrate Metabolism as Potential Drug Targets in Anisakiasis
dc.creator.none.fl_str_mv Stryiński, Robert
Mateos, Jesús
Carrera, Mónica
Jastrzebski, Jan Pawel
Bogacka, Iwona
Lopieńska-Biernat, Elzbieta
author Stryiński, Robert
author_facet Stryiński, Robert
Mateos, Jesús
Carrera, Mónica
Jastrzebski, Jan Pawel
Bogacka, Iwona
Lopieńska-Biernat, Elzbieta
author_role author
author2 Mateos, Jesús
Carrera, Mónica
Jastrzebski, Jan Pawel
Bogacka, Iwona
Lopieńska-Biernat, Elzbieta
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv National Science Centre (Poland)
European Commission
Xunta de Galicia
Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Anisakis simplex
L4 stage larvae
Intestine
Cuticle
Folliculin
Oxoglutarate dehydrogenase
Proteomics
LC-MS/MS
topic Anisakis simplex
L4 stage larvae
Intestine
Cuticle
Folliculin
Oxoglutarate dehydrogenase
Proteomics
LC-MS/MS
description 29 pages, 9 figures, 2 tables.-- This is an open access article distributed under the Creative Commons Attribution License
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/275043
url http://hdl.handle.net/10261/275043
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-103845RB-C21
The underlying dataset has been published as supplementary material of the article in the publishers platform at DOI https://doi.org/10.3390/ijms23084336
https://doi.org/10.3390/ijms23084336
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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repository.mail.fl_str_mv
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