Whi3 binds the mRNA of the G(1) cyclin CLN3 to modulate cell fate in budding yeast

Eukaryotic cells commit in G1 to a new mitotic cycle or to diverse differentiation processes. Here we show that Whi3 is a negative regulator of Cln3, a G1 cyclin that promotes transcription of many genes to trigger the G1/S transition in budding yeast. Whi3 contains an RNA-recognition motif that spe...

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Detalles Bibliográficos
Autores: Garí Marsol, Eloi, Volpe, Tom, Wang, Hongyin, Gallego, Carme, Futcher, Bruce, Aldea, Martí
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2001
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/65801
Acceso en línea:https://doi.org/10.1101/gad.203501
http://hdl.handle.net/10459.1/65801
Access Level:acceso abierto
Palabra clave:Cell cycle
G1 regulation
Differentiation
RNA-binding protein
Cyclin
mRNA localization
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spelling Whi3 binds the mRNA of the G(1) cyclin CLN3 to modulate cell fate in budding yeastGarí Marsol, EloiVolpe, TomWang, HongyinGallego, CarmeFutcher, BruceAldea, MartíCell cycleG1 regulationDifferentiationRNA-binding proteinCyclinmRNA localizationEukaryotic cells commit in G1 to a new mitotic cycle or to diverse differentiation processes. Here we show that Whi3 is a negative regulator of Cln3, a G1 cyclin that promotes transcription of many genes to trigger the G1/S transition in budding yeast. Whi3 contains an RNA-recognition motif that specifically binds the CLN3 mRNA, with no obvious effects on Cln3 levels, and localizes the CLN3 mRNA into discrete cytoplasmic foci. This is the first indication that G1 events may be regulated by locally restricting the synthesis of a cyclin. Moreover, Whi3 is also required for restraining Cln3 function in meiosis, filamentation, and mating, thus playing a key role in cell fate determination in budding yeast.We thank A. Bueno, C. Mann, S. Moreno, E. Schwob, and B. Séraphin for strains and helpful discussions, and J.X. Comella for comments on the manuscript. This work was supported by grants from the Spanish Ministry of Education and Culture, and La Paeria to E.G. and M.A., from AECI to H.W. and from the NIH to B.F. and T.V.Cold Spring Harbor Laboratory Press2001info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1101/gad.203501http://hdl.handle.net/10459.1/65801reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)InglésReproducció del document publicat a: https://doi.org/10.1101/gad.203501Genes & Development, 2001, vol. 15, núm. 21, p. 2803-8(c) Garí Marsol, Eloi et al., 2001info:eu-repo/semantics/openAccessoai:repositori.udl.cat:10459.1/658012026-06-24T12:42:17Z
dc.title.none.fl_str_mv Whi3 binds the mRNA of the G(1) cyclin CLN3 to modulate cell fate in budding yeast
title Whi3 binds the mRNA of the G(1) cyclin CLN3 to modulate cell fate in budding yeast
spellingShingle Whi3 binds the mRNA of the G(1) cyclin CLN3 to modulate cell fate in budding yeast
Garí Marsol, Eloi
Cell cycle
G1 regulation
Differentiation
RNA-binding protein
Cyclin
mRNA localization
title_short Whi3 binds the mRNA of the G(1) cyclin CLN3 to modulate cell fate in budding yeast
title_full Whi3 binds the mRNA of the G(1) cyclin CLN3 to modulate cell fate in budding yeast
title_fullStr Whi3 binds the mRNA of the G(1) cyclin CLN3 to modulate cell fate in budding yeast
title_full_unstemmed Whi3 binds the mRNA of the G(1) cyclin CLN3 to modulate cell fate in budding yeast
title_sort Whi3 binds the mRNA of the G(1) cyclin CLN3 to modulate cell fate in budding yeast
dc.creator.none.fl_str_mv Garí Marsol, Eloi
Volpe, Tom
Wang, Hongyin
Gallego, Carme
Futcher, Bruce
Aldea, Martí
author Garí Marsol, Eloi
author_facet Garí Marsol, Eloi
Volpe, Tom
Wang, Hongyin
Gallego, Carme
Futcher, Bruce
Aldea, Martí
author_role author
author2 Volpe, Tom
Wang, Hongyin
Gallego, Carme
Futcher, Bruce
Aldea, Martí
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cell cycle
G1 regulation
Differentiation
RNA-binding protein
Cyclin
mRNA localization
topic Cell cycle
G1 regulation
Differentiation
RNA-binding protein
Cyclin
mRNA localization
description Eukaryotic cells commit in G1 to a new mitotic cycle or to diverse differentiation processes. Here we show that Whi3 is a negative regulator of Cln3, a G1 cyclin that promotes transcription of many genes to trigger the G1/S transition in budding yeast. Whi3 contains an RNA-recognition motif that specifically binds the CLN3 mRNA, with no obvious effects on Cln3 levels, and localizes the CLN3 mRNA into discrete cytoplasmic foci. This is the first indication that G1 events may be regulated by locally restricting the synthesis of a cyclin. Moreover, Whi3 is also required for restraining Cln3 function in meiosis, filamentation, and mating, thus playing a key role in cell fate determination in budding yeast.
publishDate 2001
dc.date.none.fl_str_mv 2001
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1101/gad.203501
http://hdl.handle.net/10459.1/65801
url https://doi.org/10.1101/gad.203501
http://hdl.handle.net/10459.1/65801
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1101/gad.203501
Genes & Development, 2001, vol. 15, núm. 21, p. 2803-8
dc.rights.none.fl_str_mv (c) Garí Marsol, Eloi et al., 2001
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Garí Marsol, Eloi et al., 2001
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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