Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus introns

Splicing of mRNA precursors is essential in the regulation of gene expression. U2AF65 recognizes the poly-pyrimidine tract and helps in the recognition of the branch point. Inactivation of fission yeast U2AF65 (Prp2) blocks splicing of most, but not all, pre-mRNAs, for reasons that are not understoo...

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Detalles Bibliográficos
Autores: Hümmer, Stefan, Borao Arriazu, Sonia, 1993-, Guerra Moreno, Ángel, Cozzuto, Luca, Hidalgo Hernando, Elena, Ayté del Olmo, José
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/49191
Acceso en línea:http://hdl.handle.net/10230/49191
http://dx.doi.org/10.1016/j.celrep.2021.109893
Access Level:acceso abierto
Palabra clave:5′ splicing site
Prp2
U2AF65
Fission yeast
Meiosis
Splicing
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oai_identifier_str oai:repositori.upf.edu:10230/49191
network_acronym_str ES
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repository_id_str
spelling Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus intronsHümmer, StefanBorao Arriazu, Sonia, 1993-Guerra Moreno, ÁngelCozzuto, LucaHidalgo Hernando, ElenaAyté del Olmo, José5′ splicing sitePrp2U2AF65Fission yeastMeiosisSplicingSplicing of mRNA precursors is essential in the regulation of gene expression. U2AF65 recognizes the poly-pyrimidine tract and helps in the recognition of the branch point. Inactivation of fission yeast U2AF65 (Prp2) blocks splicing of most, but not all, pre-mRNAs, for reasons that are not understood. Here, we have determined genome-wide the splicing efficiency of fission yeast cells as they progress into synchronous meiosis in the presence or absence of functional Prp2. Our data indicate that in addition to the splicing elements at the 3' end of any intron, the nucleotides immediately upstream the intron will determine whether Prp2 is required or dispensable for splicing. By changing those nucleotides in any given intron, we regulate its Prp2 dependency. Our results suggest a model in which Prp2 is required for the coordinated recognition of both intronic ends, placing Prp2 as a key regulatory element in the determination of the exon-intron boundaries.This work was supported by grants from the Spanish Ministerio de Economia y Competitividad (MINECO) (BFU2015-66347-P and BFU2018-PGC2018-097248-B-I00), PLAN E and Feder, MEIONet (BFU2015-71786-REDT), and Unidad de Excelencia María de Maeztu (CEX2018-000792-M). E.H. is recipient of an ICREA Academia Award (Generalitat de Catalunya).Elsevier202120212021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/49191http://dx.doi.org/10.1016/j.celrep.2021.109893reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésCell Rep. 2021;37(4):109893info:eu-repo/grantAgreement/ES/1PE/BFU2015-66347-P© 2021 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/491912026-06-12T07:21:37Z
dc.title.none.fl_str_mv Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus introns
title Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus introns
spellingShingle Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus introns
Hümmer, Stefan
5′ splicing site
Prp2
U2AF65
Fission yeast
Meiosis
Splicing
title_short Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus introns
title_full Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus introns
title_fullStr Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus introns
title_full_unstemmed Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus introns
title_sort Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus introns
dc.creator.none.fl_str_mv Hümmer, Stefan
Borao Arriazu, Sonia, 1993-
Guerra Moreno, Ángel
Cozzuto, Luca
Hidalgo Hernando, Elena
Ayté del Olmo, José
author Hümmer, Stefan
author_facet Hümmer, Stefan
Borao Arriazu, Sonia, 1993-
Guerra Moreno, Ángel
Cozzuto, Luca
Hidalgo Hernando, Elena
Ayté del Olmo, José
author_role author
author2 Borao Arriazu, Sonia, 1993-
Guerra Moreno, Ángel
Cozzuto, Luca
Hidalgo Hernando, Elena
Ayté del Olmo, José
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv 5′ splicing site
Prp2
U2AF65
Fission yeast
Meiosis
Splicing
topic 5′ splicing site
Prp2
U2AF65
Fission yeast
Meiosis
Splicing
description Splicing of mRNA precursors is essential in the regulation of gene expression. U2AF65 recognizes the poly-pyrimidine tract and helps in the recognition of the branch point. Inactivation of fission yeast U2AF65 (Prp2) blocks splicing of most, but not all, pre-mRNAs, for reasons that are not understood. Here, we have determined genome-wide the splicing efficiency of fission yeast cells as they progress into synchronous meiosis in the presence or absence of functional Prp2. Our data indicate that in addition to the splicing elements at the 3' end of any intron, the nucleotides immediately upstream the intron will determine whether Prp2 is required or dispensable for splicing. By changing those nucleotides in any given intron, we regulate its Prp2 dependency. Our results suggest a model in which Prp2 is required for the coordinated recognition of both intronic ends, placing Prp2 as a key regulatory element in the determination of the exon-intron boundaries.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/49191
http://dx.doi.org/10.1016/j.celrep.2021.109893
url http://hdl.handle.net/10230/49191
http://dx.doi.org/10.1016/j.celrep.2021.109893
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Cell Rep. 2021;37(4):109893
info:eu-repo/grantAgreement/ES/1PE/BFU2015-66347-P
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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