Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis

Hot saline extracts of Brucella ovis were composed of vesicles with outer membrane proteins (OMPs), lipopolysaccharide, and phospholipid as constituents. Extraction with petroleum ether-chloroform-phenol yielded a protein fraction free of detectable lipopolysaccharide, in which group 3 OMPs (28,500...

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Autores: Riezu-Boj, J.I. (José Ignacio)|||/items/87349e89-7360-4cdd-81be-2e8a6174b5f6, Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b, Blasco, J.M. (J. M.)|||/items/63aa30f3-7ffa-4d2e-8dbd-d070fd4e4efa, Gamazo-de la Rasilla, C.M. (Carlos Manuel)|||/items/d6019c54-7915-4611-94c1-b772366dab1d, Diaz, R. (Ramón)|||/items/5326d716-1718-4b1a-adf2-64bb72a0861c
Tipo de recurso: artículo
Fecha de publicación:1990
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/23049
Acceso en línea:https://hdl.handle.net/10171/23049
Access Level:acceso abierto
Palabra clave:Antibodies, bacterial/analysis
Bacterial outer membrane proteins/immunology
Brucella/immunology
Brucellosis/veterinary
Sheep diseases/immunology
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spelling Antibody response to Brucella ovis outer membrane proteins in ovine brucellosisRiezu-Boj, J.I. (José Ignacio)|||/items/87349e89-7360-4cdd-81be-2e8a6174b5f6Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7bBlasco, J.M. (J. M.)|||/items/63aa30f3-7ffa-4d2e-8dbd-d070fd4e4efaGamazo-de la Rasilla, C.M. (Carlos Manuel)|||/items/d6019c54-7915-4611-94c1-b772366dab1dDiaz, R. (Ramón)|||/items/5326d716-1718-4b1a-adf2-64bb72a0861cAntibodies, bacterial/analysisBacterial outer membrane proteins/immunologyBrucella/immunologyBrucellosis/veterinarySheep diseases/immunologyHot saline extracts of Brucella ovis were composed of vesicles with outer membrane proteins (OMPs), lipopolysaccharide, and phospholipid as constituents. Extraction with petroleum ether-chloroform-phenol yielded a protein fraction free of detectable lipopolysaccharide, in which group 3 OMPs (28,500 apparent molecular weight [28.5K], 27.0K, and 25.5K) represented 81% of the total. Group 1 OMPs and 67.0K, 22.5K to 21.5K, and 19.5K to 18.0K proteins were also detected. Adsorption of immune sera with whole bacteria suggested that group 3 OMPs and 67.0K, 22.5K to 21.5K, and 19.5K to 18.0K proteins had antigenic determinants exposed on the surfaces of both B. ovis and rough B. melitensis cells but not on smooth B. melitensis cells. Antibodies to group 3 OMPs and the 67.0K protein in the sera of 93 and 87%, respectively, of B. ovis-infected rams were found by immunoblotting. Antibodies to other proteins were present in 67% of these animals. Compared with B. ovis-infected rams which had not developed lesions, rams with epididymo-orchitis had antibodies to a larger variety of proteins. Although ewes infected with B. melitensis also showed antibodies to OMPs, the immunoblot reactions were less intense.American Society for MicrobiologyDadun. Depósito Académico Digital Universidad de Navarra20122012-08-1019901990-01-0119901990-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/23049reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/230492026-06-21T12:47:57Z
dc.title.none.fl_str_mv Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis
title Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis
spellingShingle Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis
Riezu-Boj, J.I. (José Ignacio)|||/items/87349e89-7360-4cdd-81be-2e8a6174b5f6
Antibodies, bacterial/analysis
Bacterial outer membrane proteins/immunology
Brucella/immunology
Brucellosis/veterinary
Sheep diseases/immunology
title_short Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis
title_full Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis
title_fullStr Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis
title_full_unstemmed Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis
title_sort Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis
dc.creator.none.fl_str_mv Riezu-Boj, J.I. (José Ignacio)|||/items/87349e89-7360-4cdd-81be-2e8a6174b5f6
Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b
Blasco, J.M. (J. M.)|||/items/63aa30f3-7ffa-4d2e-8dbd-d070fd4e4efa
Gamazo-de la Rasilla, C.M. (Carlos Manuel)|||/items/d6019c54-7915-4611-94c1-b772366dab1d
Diaz, R. (Ramón)|||/items/5326d716-1718-4b1a-adf2-64bb72a0861c
author Riezu-Boj, J.I. (José Ignacio)|||/items/87349e89-7360-4cdd-81be-2e8a6174b5f6
author_facet Riezu-Boj, J.I. (José Ignacio)|||/items/87349e89-7360-4cdd-81be-2e8a6174b5f6
Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b
Blasco, J.M. (J. M.)|||/items/63aa30f3-7ffa-4d2e-8dbd-d070fd4e4efa
Gamazo-de la Rasilla, C.M. (Carlos Manuel)|||/items/d6019c54-7915-4611-94c1-b772366dab1d
Diaz, R. (Ramón)|||/items/5326d716-1718-4b1a-adf2-64bb72a0861c
author_role author
author2 Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b
Blasco, J.M. (J. M.)|||/items/63aa30f3-7ffa-4d2e-8dbd-d070fd4e4efa
Gamazo-de la Rasilla, C.M. (Carlos Manuel)|||/items/d6019c54-7915-4611-94c1-b772366dab1d
Diaz, R. (Ramón)|||/items/5326d716-1718-4b1a-adf2-64bb72a0861c
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Antibodies, bacterial/analysis
Bacterial outer membrane proteins/immunology
Brucella/immunology
Brucellosis/veterinary
Sheep diseases/immunology
topic Antibodies, bacterial/analysis
Bacterial outer membrane proteins/immunology
Brucella/immunology
Brucellosis/veterinary
Sheep diseases/immunology
description Hot saline extracts of Brucella ovis were composed of vesicles with outer membrane proteins (OMPs), lipopolysaccharide, and phospholipid as constituents. Extraction with petroleum ether-chloroform-phenol yielded a protein fraction free of detectable lipopolysaccharide, in which group 3 OMPs (28,500 apparent molecular weight [28.5K], 27.0K, and 25.5K) represented 81% of the total. Group 1 OMPs and 67.0K, 22.5K to 21.5K, and 19.5K to 18.0K proteins were also detected. Adsorption of immune sera with whole bacteria suggested that group 3 OMPs and 67.0K, 22.5K to 21.5K, and 19.5K to 18.0K proteins had antigenic determinants exposed on the surfaces of both B. ovis and rough B. melitensis cells but not on smooth B. melitensis cells. Antibodies to group 3 OMPs and the 67.0K protein in the sera of 93 and 87%, respectively, of B. ovis-infected rams were found by immunoblotting. Antibodies to other proteins were present in 67% of these animals. Compared with B. ovis-infected rams which had not developed lesions, rams with epididymo-orchitis had antibodies to a larger variety of proteins. Although ewes infected with B. melitensis also showed antibodies to OMPs, the immunoblot reactions were less intense.
publishDate 1990
dc.date.none.fl_str_mv 1990
1990-01-01
1990
1990-01-01
2012
2012-08-10
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/23049
url https://hdl.handle.net/10171/23049
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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