The secreted autotransporter toxin (Sat) does not act as a virulence factor in the probiotic Escherichia coli strain Nissle 1917

BACKGROUND: Escherichia coli Nissle 1917 (EcN) is a probiotic used in the treatment of intestinal diseases. Although it is considered safe, EcN is closely related to the uropathogenic E. coli strain CFT073 and contains many of its predicted virulence elements. Thus, it is relevant to assess whether...

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Autores: Toloza Maturana, Lorena, Giménez Claudio, Rosa, Fábrega Fernández, María José, Alvarez Villagomez, Carina Shianya, Aguilera Gil, Maria Laura, Cañas Pacheco, María Alexandra, Martín Venegas, Raquel, Badía Palacín, Josefa, Baldomà Llavinés, Laura
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/68591
Acceso en línea:https://hdl.handle.net/2445/68591
Access Level:acceso abierto
Palabra clave:Escheríchia coli
Intestins
Probiòtics
Escherichia coli
Intestines
Probiotics
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spelling The secreted autotransporter toxin (Sat) does not act as a virulence factor in the probiotic Escherichia coli strain Nissle 1917Toloza Maturana, LorenaGiménez Claudio, RosaFábrega Fernández, María JoséAlvarez Villagomez, Carina ShianyaAguilera Gil, Maria LauraCañas Pacheco, María AlexandraMartín Venegas, RaquelBadía Palacín, JosefaBaldomà Llavinés, LauraEscheríchia coliIntestinsProbiòticsEscherichia coliIntestinesProbioticsBACKGROUND: Escherichia coli Nissle 1917 (EcN) is a probiotic used in the treatment of intestinal diseases. Although it is considered safe, EcN is closely related to the uropathogenic E. coli strain CFT073 and contains many of its predicted virulence elements. Thus, it is relevant to assess whether virulence-associated genes are functional in EcN. One of these genes encodes the secreted autotransporter toxin (Sat), a member of the serine protease autotransporters of Enterobacteriaceae (SPATEs) that are secreted following the type V autotransporter pathway. Sat is highly prevalent in certain E. coli pathogenic groups responsible for urinary and intestinal infections. In these pathogens Sat promotes cytotoxic effects in several lines of undifferentiated epithelial cells, but not in differentiated Caco-2 cells. RESULTS: Here we provide evidence that sat is expressed by EcN during the colonization of mouse intestine. The EcN protein is secreted as an active serine protease, with its 107 kDa-passenger domain released into the medium as a soluble protein. Expression of recombinant EcN Sat protein in strain HB101 increases paracellular permeability to mannitol in polarized Caco-2 monolayers. This effect, also reported for the Sat protein of diffusely adherent E. coli, is not observed when this protein is expressed in the EcN background. In addition, we show that EcN supernatants confer protection against Sat-mediated effects on paracellular permeability, thus indicating that other secreted EcN factors are able to prevent barrier disruption caused by pathogen-related factors. Sat is not required for intestinal colonization, but the EcNsat::cat mutant outcompetes wild-type EcN in the streptomycin-treated mouse model. Analysis of the presence of sat in 29 strains of the ECOR collection isolated from stools of healthy humans shows 34.8 % positives, with high prevalence of strains of the phylogenetic groups D and B2, related with extra-intestinal infections. CONCLUSIONS: Sat does not act as a virulence factor in EcN. The role of Sat in intestinal pathogenesis relies on other genetic determinants responsible for the bacterial pathotype.BioMed Central2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/68591Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1186/s12866-015-0591-5BMC Microbiology, 2015, vol. 15, p. 250http://dx.doi.org/10.1186/s12866-015-0591-5cc-by (c) Toloza Maturana, Lorena et al., 2015http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/685912025-07-25T16:43:42Z
dc.title.none.fl_str_mv The secreted autotransporter toxin (Sat) does not act as a virulence factor in the probiotic Escherichia coli strain Nissle 1917
title The secreted autotransporter toxin (Sat) does not act as a virulence factor in the probiotic Escherichia coli strain Nissle 1917
spellingShingle The secreted autotransporter toxin (Sat) does not act as a virulence factor in the probiotic Escherichia coli strain Nissle 1917
Toloza Maturana, Lorena
Escheríchia coli
Intestins
Probiòtics
Escherichia coli
Intestines
Probiotics
title_short The secreted autotransporter toxin (Sat) does not act as a virulence factor in the probiotic Escherichia coli strain Nissle 1917
title_full The secreted autotransporter toxin (Sat) does not act as a virulence factor in the probiotic Escherichia coli strain Nissle 1917
title_fullStr The secreted autotransporter toxin (Sat) does not act as a virulence factor in the probiotic Escherichia coli strain Nissle 1917
title_full_unstemmed The secreted autotransporter toxin (Sat) does not act as a virulence factor in the probiotic Escherichia coli strain Nissle 1917
title_sort The secreted autotransporter toxin (Sat) does not act as a virulence factor in the probiotic Escherichia coli strain Nissle 1917
dc.creator.none.fl_str_mv Toloza Maturana, Lorena
Giménez Claudio, Rosa
Fábrega Fernández, María José
Alvarez Villagomez, Carina Shianya
Aguilera Gil, Maria Laura
Cañas Pacheco, María Alexandra
Martín Venegas, Raquel
Badía Palacín, Josefa
Baldomà Llavinés, Laura
author Toloza Maturana, Lorena
author_facet Toloza Maturana, Lorena
Giménez Claudio, Rosa
Fábrega Fernández, María José
Alvarez Villagomez, Carina Shianya
Aguilera Gil, Maria Laura
Cañas Pacheco, María Alexandra
Martín Venegas, Raquel
Badía Palacín, Josefa
Baldomà Llavinés, Laura
author_role author
author2 Giménez Claudio, Rosa
Fábrega Fernández, María José
Alvarez Villagomez, Carina Shianya
Aguilera Gil, Maria Laura
Cañas Pacheco, María Alexandra
Martín Venegas, Raquel
Badía Palacín, Josefa
Baldomà Llavinés, Laura
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Escheríchia coli
Intestins
Probiòtics
Escherichia coli
Intestines
Probiotics
topic Escheríchia coli
Intestins
Probiòtics
Escherichia coli
Intestines
Probiotics
description BACKGROUND: Escherichia coli Nissle 1917 (EcN) is a probiotic used in the treatment of intestinal diseases. Although it is considered safe, EcN is closely related to the uropathogenic E. coli strain CFT073 and contains many of its predicted virulence elements. Thus, it is relevant to assess whether virulence-associated genes are functional in EcN. One of these genes encodes the secreted autotransporter toxin (Sat), a member of the serine protease autotransporters of Enterobacteriaceae (SPATEs) that are secreted following the type V autotransporter pathway. Sat is highly prevalent in certain E. coli pathogenic groups responsible for urinary and intestinal infections. In these pathogens Sat promotes cytotoxic effects in several lines of undifferentiated epithelial cells, but not in differentiated Caco-2 cells. RESULTS: Here we provide evidence that sat is expressed by EcN during the colonization of mouse intestine. The EcN protein is secreted as an active serine protease, with its 107 kDa-passenger domain released into the medium as a soluble protein. Expression of recombinant EcN Sat protein in strain HB101 increases paracellular permeability to mannitol in polarized Caco-2 monolayers. This effect, also reported for the Sat protein of diffusely adherent E. coli, is not observed when this protein is expressed in the EcN background. In addition, we show that EcN supernatants confer protection against Sat-mediated effects on paracellular permeability, thus indicating that other secreted EcN factors are able to prevent barrier disruption caused by pathogen-related factors. Sat is not required for intestinal colonization, but the EcNsat::cat mutant outcompetes wild-type EcN in the streptomycin-treated mouse model. Analysis of the presence of sat in 29 strains of the ECOR collection isolated from stools of healthy humans shows 34.8 % positives, with high prevalence of strains of the phylogenetic groups D and B2, related with extra-intestinal infections. CONCLUSIONS: Sat does not act as a virulence factor in EcN. The role of Sat in intestinal pathogenesis relies on other genetic determinants responsible for the bacterial pathotype.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/68591
url https://hdl.handle.net/2445/68591
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1186/s12866-015-0591-5
BMC Microbiology, 2015, vol. 15, p. 250
http://dx.doi.org/10.1186/s12866-015-0591-5
dc.rights.none.fl_str_mv cc-by (c) Toloza Maturana, Lorena et al., 2015
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Toloza Maturana, Lorena et al., 2015
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,647109

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