Cold Plasma-Treated Ringer's Saline: A Weapon to Target Osteosarcoma.

Osteosarcoma (OS) is the main primary bone cancer, presenting poor prognosis and difficult treatment. An innovative therapy may be found in cold plasmas, which show anti-cancer effects related to the generation of reactive oxygen and nitrogen species in liquids. In vitro models are based on the effe...

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Autores: Mateu-Sanz M, Tornín J, Brulin B, Khlyustova A, Ginebra MP, Layrolle P, Canal C
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Fundació Sant Joan de Déu
Repositorio:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
OAI Identifier:oai:fsjd.fundanetsuite.com:p17319
Acceso en línea:https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=17319
Access Level:acceso abierto
Palabra clave:Ringer’s saline
bone cancer
cold atmospheric plasma
organotypic model
osteosarcoma
plasma-activated liquid
reactive species
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spelling Cold Plasma-Treated Ringer's Saline: A Weapon to Target Osteosarcoma.Mateu-Sanz MTornín JBrulin BKhlyustova AGinebra MPLayrolle PCanal CRinger’s salinebone cancercold atmospheric plasmaorganotypic modelosteosarcomaplasma-activated liquidreactive speciesOsteosarcoma (OS) is the main primary bone cancer, presenting poor prognosis and difficult treatment. An innovative therapy may be found in cold plasmas, which show anti-cancer effects related to the generation of reactive oxygen and nitrogen species in liquids. In vitro models are based on the effects of plasma-treated culture media on cell cultures. However, effects of plasma-activated saline solutions with clinical application have not yet been explored in OS. The aim of this study is to obtain mechanistic insights on the action of plasma-activated Ringer's saline (PAR) for OS therapy in cell and organotypic cultures. To that aim, cold atmospheric plasma jets were used to obtain PAR, which produced cytotoxic effects in human OS cells (SaOS-2, MG-63, and U2-OS), related to the increasing concentration of reactive oxygen and nitrogen species generated. Proof of selectivity was found in the sustained viability of hBM-MSCs with the same treatments. Organotypic cultures of murine OS confirmed the time-dependent cytotoxicity observed in 2D. Histological analysis showed a decrease in proliferating cells (lower Ki-67 expression). It is shown that the selectivity of PAR is highly dependent on the concentrations of reactive species, being the differential intracellular reactive oxygen species increase and DNA damage between OS cells and hBM-MSCs key mediators for cell apoptosis.MDPI2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=17319CancersISSN: 20726694reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déuinstname:Fundació Sant Joan de DéuInglésinfo:eu-repo/semantics/openAccessoai:fsjd.fundanetsuite.com:p173192026-05-27T12:37:41Z
dc.title.none.fl_str_mv Cold Plasma-Treated Ringer's Saline: A Weapon to Target Osteosarcoma.
title Cold Plasma-Treated Ringer's Saline: A Weapon to Target Osteosarcoma.
spellingShingle Cold Plasma-Treated Ringer's Saline: A Weapon to Target Osteosarcoma.
Mateu-Sanz M
Ringer’s saline
bone cancer
cold atmospheric plasma
organotypic model
osteosarcoma
plasma-activated liquid
reactive species
title_short Cold Plasma-Treated Ringer's Saline: A Weapon to Target Osteosarcoma.
title_full Cold Plasma-Treated Ringer's Saline: A Weapon to Target Osteosarcoma.
title_fullStr Cold Plasma-Treated Ringer's Saline: A Weapon to Target Osteosarcoma.
title_full_unstemmed Cold Plasma-Treated Ringer's Saline: A Weapon to Target Osteosarcoma.
title_sort Cold Plasma-Treated Ringer's Saline: A Weapon to Target Osteosarcoma.
dc.creator.none.fl_str_mv Mateu-Sanz M
Tornín J
Brulin B
Khlyustova A
Ginebra MP
Layrolle P
Canal C
author Mateu-Sanz M
author_facet Mateu-Sanz M
Tornín J
Brulin B
Khlyustova A
Ginebra MP
Layrolle P
Canal C
author_role author
author2 Tornín J
Brulin B
Khlyustova A
Ginebra MP
Layrolle P
Canal C
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ringer’s saline
bone cancer
cold atmospheric plasma
organotypic model
osteosarcoma
plasma-activated liquid
reactive species
topic Ringer’s saline
bone cancer
cold atmospheric plasma
organotypic model
osteosarcoma
plasma-activated liquid
reactive species
description Osteosarcoma (OS) is the main primary bone cancer, presenting poor prognosis and difficult treatment. An innovative therapy may be found in cold plasmas, which show anti-cancer effects related to the generation of reactive oxygen and nitrogen species in liquids. In vitro models are based on the effects of plasma-treated culture media on cell cultures. However, effects of plasma-activated saline solutions with clinical application have not yet been explored in OS. The aim of this study is to obtain mechanistic insights on the action of plasma-activated Ringer's saline (PAR) for OS therapy in cell and organotypic cultures. To that aim, cold atmospheric plasma jets were used to obtain PAR, which produced cytotoxic effects in human OS cells (SaOS-2, MG-63, and U2-OS), related to the increasing concentration of reactive oxygen and nitrogen species generated. Proof of selectivity was found in the sustained viability of hBM-MSCs with the same treatments. Organotypic cultures of murine OS confirmed the time-dependent cytotoxicity observed in 2D. Histological analysis showed a decrease in proliferating cells (lower Ki-67 expression). It is shown that the selectivity of PAR is highly dependent on the concentrations of reactive species, being the differential intracellular reactive oxygen species increase and DNA damage between OS cells and hBM-MSCs key mediators for cell apoptosis.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=17319
url https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=17319
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Cancers
ISSN: 20726694
reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
instname:Fundació Sant Joan de Déu
instname_str Fundació Sant Joan de Déu
reponame_str r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
collection r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
repository.name.fl_str_mv
repository.mail.fl_str_mv
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