CfrA, a Novel Carbon Flow Regulator, Adapts Carbon Metabolism to Nitrogen Deficiency in Cyanobacteria

Cyanobacteria unable to fix atmospheric nitrogen have evolved sophisticated adaptations to survive to long periods of nitrogen starvation. These genetic programs are still largely unknown¿as evidenced by the many proteins whose expression is regulated in response to nitrogen availability, but which...

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Autores: Muro-Pastor, M. Isabel, Cutillas-Farray, Áureo, Pérez-Rodríguez, Laura, Pérez-Saavedra, Julia, Vega-de Armas, Ana, Paredes, Ana, Robles-Rengel, Rocío, Florencio, Francisco J.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/228534
Acceso en línea:http://hdl.handle.net/10261/228534
Access Level:acceso abierto
Palabra clave:Carbon metabolism
CfrA
Chlorosis
Glycogen
Nitrogen starvation
Synechocystis
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spelling CfrA, a Novel Carbon Flow Regulator, Adapts Carbon Metabolism to Nitrogen Deficiency in CyanobacteriaMuro-Pastor, M. IsabelCutillas-Farray, ÁureoPérez-Rodríguez, LauraPérez-Saavedra, JuliaVega-de Armas, AnaParedes, AnaRobles-Rengel, RocíoFlorencio, Francisco J.Carbon metabolismCfrAChlorosisGlycogenNitrogen starvationSynechocystisCyanobacteria unable to fix atmospheric nitrogen have evolved sophisticated adaptations to survive to long periods of nitrogen starvation. These genetic programs are still largely unknown¿as evidenced by the many proteins whose expression is regulated in response to nitrogen availability, but which belong to unknown or hypothetical categories. In Synechocystis sp. PCC 6803, the global nitrogen regulator NtcA activates the expression of the sll0944 gene upon nitrogen deprivation. This gene encodes a protein that is highly conserved in cyanobacteria, but of unknown function. Based on the results described herein, we named the product of sll0944 carbon flow regulator A (CfrA). We analyzed the phenotypes of strains containing different levels of CfrA, including a knock-out strain (DcfrA), and two strains overexpressing CfrA from either the constitutive Ptrc promoter (Ptrc-cfrA) or the arsenite-inducible promoter ParsB (Pars-cfrA). Our results show that the amount of CfrA determines the accumulation of glycogen, and affects the synthesis of protein and photosynthetic pigments as well as amino acid pools. Strains with high levels of CfrA present high levels of glycogen and a decrease in photosynthetic pigments and protein content when nitrogen is available. Possible interactions between CfrA and the pyruvate dehydrogenase complex or PII protein have been revealed. The phenotype associated with CfrA overexpression is also observed in PII-deficient strains; however, it is lethal in this genetic background. Taken together, our results indicate a role for CfrA in the adaptation of carbon flux during acclimation to nitrogen deficiency.American Society of Plant BiologistsMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/228534reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2013-41712http://dx.doi.org/10.1104/pp.20.00802Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2285342026-05-22T06:33:51Z
dc.title.none.fl_str_mv CfrA, a Novel Carbon Flow Regulator, Adapts Carbon Metabolism to Nitrogen Deficiency in Cyanobacteria
title CfrA, a Novel Carbon Flow Regulator, Adapts Carbon Metabolism to Nitrogen Deficiency in Cyanobacteria
spellingShingle CfrA, a Novel Carbon Flow Regulator, Adapts Carbon Metabolism to Nitrogen Deficiency in Cyanobacteria
Muro-Pastor, M. Isabel
Carbon metabolism
CfrA
Chlorosis
Glycogen
Nitrogen starvation
Synechocystis
title_short CfrA, a Novel Carbon Flow Regulator, Adapts Carbon Metabolism to Nitrogen Deficiency in Cyanobacteria
title_full CfrA, a Novel Carbon Flow Regulator, Adapts Carbon Metabolism to Nitrogen Deficiency in Cyanobacteria
title_fullStr CfrA, a Novel Carbon Flow Regulator, Adapts Carbon Metabolism to Nitrogen Deficiency in Cyanobacteria
title_full_unstemmed CfrA, a Novel Carbon Flow Regulator, Adapts Carbon Metabolism to Nitrogen Deficiency in Cyanobacteria
title_sort CfrA, a Novel Carbon Flow Regulator, Adapts Carbon Metabolism to Nitrogen Deficiency in Cyanobacteria
dc.creator.none.fl_str_mv Muro-Pastor, M. Isabel
Cutillas-Farray, Áureo
Pérez-Rodríguez, Laura
Pérez-Saavedra, Julia
Vega-de Armas, Ana
Paredes, Ana
Robles-Rengel, Rocío
Florencio, Francisco J.
author Muro-Pastor, M. Isabel
author_facet Muro-Pastor, M. Isabel
Cutillas-Farray, Áureo
Pérez-Rodríguez, Laura
Pérez-Saavedra, Julia
Vega-de Armas, Ana
Paredes, Ana
Robles-Rengel, Rocío
Florencio, Francisco J.
author_role author
author2 Cutillas-Farray, Áureo
Pérez-Rodríguez, Laura
Pérez-Saavedra, Julia
Vega-de Armas, Ana
Paredes, Ana
Robles-Rengel, Rocío
Florencio, Francisco J.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Carbon metabolism
CfrA
Chlorosis
Glycogen
Nitrogen starvation
Synechocystis
topic Carbon metabolism
CfrA
Chlorosis
Glycogen
Nitrogen starvation
Synechocystis
description Cyanobacteria unable to fix atmospheric nitrogen have evolved sophisticated adaptations to survive to long periods of nitrogen starvation. These genetic programs are still largely unknown¿as evidenced by the many proteins whose expression is regulated in response to nitrogen availability, but which belong to unknown or hypothetical categories. In Synechocystis sp. PCC 6803, the global nitrogen regulator NtcA activates the expression of the sll0944 gene upon nitrogen deprivation. This gene encodes a protein that is highly conserved in cyanobacteria, but of unknown function. Based on the results described herein, we named the product of sll0944 carbon flow regulator A (CfrA). We analyzed the phenotypes of strains containing different levels of CfrA, including a knock-out strain (DcfrA), and two strains overexpressing CfrA from either the constitutive Ptrc promoter (Ptrc-cfrA) or the arsenite-inducible promoter ParsB (Pars-cfrA). Our results show that the amount of CfrA determines the accumulation of glycogen, and affects the synthesis of protein and photosynthetic pigments as well as amino acid pools. Strains with high levels of CfrA present high levels of glycogen and a decrease in photosynthetic pigments and protein content when nitrogen is available. Possible interactions between CfrA and the pyruvate dehydrogenase complex or PII protein have been revealed. The phenotype associated with CfrA overexpression is also observed in PII-deficient strains; however, it is lethal in this genetic background. Taken together, our results indicate a role for CfrA in the adaptation of carbon flux during acclimation to nitrogen deficiency.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/228534
url http://hdl.handle.net/10261/228534
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2013-41712
http://dx.doi.org/10.1104/pp.20.00802
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society of Plant Biologists
publisher.none.fl_str_mv American Society of Plant Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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