Globotriaosylceramide-related biomarkers of fabry disease identified in plasma by high-performance thin-layer chromatography - densitometry - mass spectrometry

6 figures, 2 tables.-- Supplementary information available.-- © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/

Detalles Bibliográficos
Autores: Jarne, Carmen, Membrado, Luis, Savirón, María, Vela, Jesús, Orduna, Jesús, Garriga, Rosa, Galbán, Javier, Cebolla, Vicente L.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/227405
Acceso en línea:http://hdl.handle.net/10261/227405
Access Level:acceso abierto
Palabra clave:HPTLC
HPTLC-ESI-MS
HPTLC-APCI-MS
AMD
Orcinol derivatization
Scanning densitometry
Sphingolipids
Globotriaosylceramide
Fabry's disease
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spelling Globotriaosylceramide-related biomarkers of fabry disease identified in plasma by high-performance thin-layer chromatography - densitometry - mass spectrometryJarne, CarmenMembrado, LuisSavirón, MaríaVela, JesúsOrduna, JesúsGarriga, RosaGalbán, JavierCebolla, Vicente L.HPTLCHPTLC-ESI-MSHPTLC-APCI-MSAMDOrcinol derivatizationScanning densitometrySphingolipidsGlobotriaosylceramideFabry's disease6 figures, 2 tables.-- Supplementary information available.-- © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/Identification of 19 molecular species of globotriaosylceramides (Gb3) in extracts from a Fabry's plasma patient and a healthy control was performed by High-Performance Thin-Layer Chromatography (HPTLC)-densitometry and online coupling to Mass Spectrometry (MS). Separation was carried out on LiChrospher plates using Automated Multiple Development (AMD). Densitometry was performed on twin plates by combining detection in the visible at 550 nm, through previous on-plate orcinol derivatization, and by Ultraviolet 190 nm, using a non-impregnated plate. The latter was directly coupled to an ion-trap mass spectrometer through an automated elution-based interface. Gb3 molecular species, which were identified by HPTLC- Electrospray Mass Spectrometry (+)-MS and confirmed by MS/MS or HPTLC-Atmospheric Pressure Chemical Ionization Mass Spectrometry (+)-MS, are: five isoforms of saturated Gb3; seven isoforms of methylated Gb3; and seven species with two additional double bonds. Twelve of these species were previously reported as biomarkers of Fabry's lysosomal disorder using a Liquid Chromatography-MS-based method, and the other seven are structurally similar, closely related to them. Saturated Gb3 isoforms migrated on LiChrospher plate in one of the separated peaks corresponding to the migration zone of ceramide trihexosides standard. Instead, methylated and unsaturated Gb3 species co-migrated with sphingomyelin species. Ion intensity ESI-MS profiles show that saturated Gb3 species in Fabry's plasma were in higher concentration than in control sample. Before applying the Thin-Layer Chromatography (TLC)-MS interface on HPTLC separated peaks, its positioning precision was first studied using ceramide tri-hexosides as model compound. This provided information on Gb3 peak broadening and splitting during its migration.This work was supported by DGA-FEDER (E25_17R, N&SB). One of us (J.G.) thanks to Spanish Plan Nacional I+D+i (CTQ2016-76846-R project).Peer reviewedElsevier BVGobierno de AragónEuropean CommissionMinisterio de Economía, Industria y Competitividad (España)Jarne, Carmen [0000-0002-9047-7069]Membrado, Luis [0000-0001-6435-7525]Savirón, María [0000-0001-7299-0187]Vela, Jesús [0000-0002-4887-1652]Orduna, Jesús [0000-0003-3514-2570]Garriga, Rosa [0000-0003-2607-7834]Galbán, Javier [0000-0002-8973-5104]Cebolla, Vicente L. [0000-0002-9786-9217]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/227405reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2016-76846-Rhttp://dx.doi.org/10.1016/j.chroma.2021.461895Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2274052026-05-22T06:33:51Z
dc.title.none.fl_str_mv Globotriaosylceramide-related biomarkers of fabry disease identified in plasma by high-performance thin-layer chromatography - densitometry - mass spectrometry
title Globotriaosylceramide-related biomarkers of fabry disease identified in plasma by high-performance thin-layer chromatography - densitometry - mass spectrometry
spellingShingle Globotriaosylceramide-related biomarkers of fabry disease identified in plasma by high-performance thin-layer chromatography - densitometry - mass spectrometry
Jarne, Carmen
HPTLC
HPTLC-ESI-MS
HPTLC-APCI-MS
AMD
Orcinol derivatization
Scanning densitometry
Sphingolipids
Globotriaosylceramide
Fabry's disease
title_short Globotriaosylceramide-related biomarkers of fabry disease identified in plasma by high-performance thin-layer chromatography - densitometry - mass spectrometry
title_full Globotriaosylceramide-related biomarkers of fabry disease identified in plasma by high-performance thin-layer chromatography - densitometry - mass spectrometry
title_fullStr Globotriaosylceramide-related biomarkers of fabry disease identified in plasma by high-performance thin-layer chromatography - densitometry - mass spectrometry
title_full_unstemmed Globotriaosylceramide-related biomarkers of fabry disease identified in plasma by high-performance thin-layer chromatography - densitometry - mass spectrometry
title_sort Globotriaosylceramide-related biomarkers of fabry disease identified in plasma by high-performance thin-layer chromatography - densitometry - mass spectrometry
dc.creator.none.fl_str_mv Jarne, Carmen
Membrado, Luis
Savirón, María
Vela, Jesús
Orduna, Jesús
Garriga, Rosa
Galbán, Javier
Cebolla, Vicente L.
author Jarne, Carmen
author_facet Jarne, Carmen
Membrado, Luis
Savirón, María
Vela, Jesús
Orduna, Jesús
Garriga, Rosa
Galbán, Javier
Cebolla, Vicente L.
author_role author
author2 Membrado, Luis
Savirón, María
Vela, Jesús
Orduna, Jesús
Garriga, Rosa
Galbán, Javier
Cebolla, Vicente L.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Gobierno de Aragón
European Commission
Ministerio de Economía, Industria y Competitividad (España)
Jarne, Carmen [0000-0002-9047-7069]
Membrado, Luis [0000-0001-6435-7525]
Savirón, María [0000-0001-7299-0187]
Vela, Jesús [0000-0002-4887-1652]
Orduna, Jesús [0000-0003-3514-2570]
Garriga, Rosa [0000-0003-2607-7834]
Galbán, Javier [0000-0002-8973-5104]
Cebolla, Vicente L. [0000-0002-9786-9217]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv HPTLC
HPTLC-ESI-MS
HPTLC-APCI-MS
AMD
Orcinol derivatization
Scanning densitometry
Sphingolipids
Globotriaosylceramide
Fabry's disease
topic HPTLC
HPTLC-ESI-MS
HPTLC-APCI-MS
AMD
Orcinol derivatization
Scanning densitometry
Sphingolipids
Globotriaosylceramide
Fabry's disease
description 6 figures, 2 tables.-- Supplementary information available.-- © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/227405
url http://hdl.handle.net/10261/227405
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2016-76846-R
http://dx.doi.org/10.1016/j.chroma.2021.461895
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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