Polysialic and colanic acids metabolism in Escherichia coli K92 is regulated by RcsA and RcsB
[EN]We have shown previously that Escherichia coli K92 produces two different capsular polymers known as CA (colanic acid) and PA (polysialic acid) in a thermoregulated manner. The complex Rcs phosphorelay is largely related to the regulation of CA synthesis. Through deletion of rscA and rscB genes,...
| Autores: | , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | España |
| Recursos: | Universidad de León |
| Repositorio: | BULERIA. Repositorio Institucional de la Universidad de León |
| OAI Identifier: | oai:buleria.unileon.es:10612/24861 |
| Acesso em linha: | https://portlandpress.com/bioscirep/article/33/3/e00038/56015/Polysialic-and-colanic-acids-metabolism-in https://hdl.handle.net/10612/24861 |
| Access Level: | acceso abierto |
| Palavra-chave: | Biología Bioquímica Capsular polysialic acid Colanic acid qRT–PCR RcsA and RcsB regulation 2302.21 Biología Molecular 2414 Microbiología 2302 Bioquímica |
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Polysialic and colanic acids metabolism in Escherichia coli K92 is regulated by RcsA and RcsBNavasa Mayo, NicolásRodríguez Aparicio, Leandro BenitoFerrero García, Miguel ÁngelMonteagudo Mera, AndreaMartínez Blanco, HonorinaBiologíaBioquímicaCapsular polysialic acidColanic acidqRT–PCRRcsA and RcsB regulation2302.21 Biología Molecular2414 Microbiología2302 Bioquímica[EN]We have shown previously that Escherichia coli K92 produces two different capsular polymers known as CA (colanic acid) and PA (polysialic acid) in a thermoregulated manner. The complex Rcs phosphorelay is largely related to the regulation of CA synthesis. Through deletion of rscA and rscB genes, we show that the Rcs system is involved in the regulation of both CA and PA synthesis in E. coli K92. Deletion of either rcsA or rcsB genes resulted in decreased expression of cps (CA biosynthesis cluster) at 19°C and 37°C, but only CA production was reduced at 19°C. Concerning PA, both deletions enhanced its synthesis at 37°C, which does not correlate with the reduced kps (PA biosynthesis cluster) expression observed in the rcsB mutant. Under this condition, expression of the nan operon responsible for PA catabolism was greatly reduced. Although RcsA and RcsB acted as negative regulators of PA synthesis at 37°C, their absence did not reestablish PA expression at low temperatures, despite the deletion of rcsB resulting in enhanced kps expression. Finally, our results revealed that RcsB controlled the expression of several genes (dsrA, rfaH, h-ns and slyA) involved in the thermoregulation of CA and PA synthesis, indicating that RcsB is part of a complex regulatory mechanism governing the surface appearance in E. coli.SIThis work was supported the Direccion General de Investigaci´on [grant number AGL2007-62428] and Junta de Castilla y León [grant number JCyL 32A08].Portland PressBiochemical SocietyBioquimica y Biologia MolecularFacultad de Veterinaria2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://portlandpress.com/bioscirep/article/33/3/e00038/56015/Polysialic-and-colanic-acids-metabolism-inhttps://hdl.handle.net/10612/24861reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo:eu-repo/grantAgreement/MEC//AGL2007-62428http://creativecommons.org/licenses/by/3.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/248612026-06-24T12:43:27Z |
| dc.title.none.fl_str_mv |
Polysialic and colanic acids metabolism in Escherichia coli K92 is regulated by RcsA and RcsB |
| title |
Polysialic and colanic acids metabolism in Escherichia coli K92 is regulated by RcsA and RcsB |
| spellingShingle |
Polysialic and colanic acids metabolism in Escherichia coli K92 is regulated by RcsA and RcsB Navasa Mayo, Nicolás Biología Bioquímica Capsular polysialic acid Colanic acid qRT–PCR RcsA and RcsB regulation 2302.21 Biología Molecular 2414 Microbiología 2302 Bioquímica |
| title_short |
Polysialic and colanic acids metabolism in Escherichia coli K92 is regulated by RcsA and RcsB |
| title_full |
Polysialic and colanic acids metabolism in Escherichia coli K92 is regulated by RcsA and RcsB |
| title_fullStr |
Polysialic and colanic acids metabolism in Escherichia coli K92 is regulated by RcsA and RcsB |
| title_full_unstemmed |
Polysialic and colanic acids metabolism in Escherichia coli K92 is regulated by RcsA and RcsB |
| title_sort |
Polysialic and colanic acids metabolism in Escherichia coli K92 is regulated by RcsA and RcsB |
| dc.creator.none.fl_str_mv |
Navasa Mayo, Nicolás Rodríguez Aparicio, Leandro Benito Ferrero García, Miguel Ángel Monteagudo Mera, Andrea Martínez Blanco, Honorina |
| author |
Navasa Mayo, Nicolás |
| author_facet |
Navasa Mayo, Nicolás Rodríguez Aparicio, Leandro Benito Ferrero García, Miguel Ángel Monteagudo Mera, Andrea Martínez Blanco, Honorina |
| author_role |
author |
| author2 |
Rodríguez Aparicio, Leandro Benito Ferrero García, Miguel Ángel Monteagudo Mera, Andrea Martínez Blanco, Honorina |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Bioquimica y Biologia Molecular Facultad de Veterinaria |
| dc.subject.none.fl_str_mv |
Biología Bioquímica Capsular polysialic acid Colanic acid qRT–PCR RcsA and RcsB regulation 2302.21 Biología Molecular 2414 Microbiología 2302 Bioquímica |
| topic |
Biología Bioquímica Capsular polysialic acid Colanic acid qRT–PCR RcsA and RcsB regulation 2302.21 Biología Molecular 2414 Microbiología 2302 Bioquímica |
| description |
[EN]We have shown previously that Escherichia coli K92 produces two different capsular polymers known as CA (colanic acid) and PA (polysialic acid) in a thermoregulated manner. The complex Rcs phosphorelay is largely related to the regulation of CA synthesis. Through deletion of rscA and rscB genes, we show that the Rcs system is involved in the regulation of both CA and PA synthesis in E. coli K92. Deletion of either rcsA or rcsB genes resulted in decreased expression of cps (CA biosynthesis cluster) at 19°C and 37°C, but only CA production was reduced at 19°C. Concerning PA, both deletions enhanced its synthesis at 37°C, which does not correlate with the reduced kps (PA biosynthesis cluster) expression observed in the rcsB mutant. Under this condition, expression of the nan operon responsible for PA catabolism was greatly reduced. Although RcsA and RcsB acted as negative regulators of PA synthesis at 37°C, their absence did not reestablish PA expression at low temperatures, despite the deletion of rcsB resulting in enhanced kps expression. Finally, our results revealed that RcsB controlled the expression of several genes (dsrA, rfaH, h-ns and slyA) involved in the thermoregulation of CA and PA synthesis, indicating that RcsB is part of a complex regulatory mechanism governing the surface appearance in E. coli. |
| publishDate |
2013 |
| dc.date.none.fl_str_mv |
2013 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://portlandpress.com/bioscirep/article/33/3/e00038/56015/Polysialic-and-colanic-acids-metabolism-in https://hdl.handle.net/10612/24861 |
| url |
https://portlandpress.com/bioscirep/article/33/3/e00038/56015/Polysialic-and-colanic-acids-metabolism-in https://hdl.handle.net/10612/24861 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
info:eu-repo/grantAgreement/MEC//AGL2007-62428 |
| dc.rights.none.fl_str_mv |
http://creativecommons.org/licenses/by/3.0/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by/3.0/ |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Portland Press Biochemical Society |
| publisher.none.fl_str_mv |
Portland Press Biochemical Society |
| dc.source.none.fl_str_mv |
reponame:BULERIA. Repositorio Institucional de la Universidad de León instname:Universidad de León |
| instname_str |
Universidad de León |
| reponame_str |
BULERIA. Repositorio Institucional de la Universidad de León |
| collection |
BULERIA. Repositorio Institucional de la Universidad de León |
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| repository.mail.fl_str_mv |
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1869417673641689088 |
| score |
15,811543 |