Novel pH-stable glycoside hydrolase family 3 β-xylosidase from Talaromyces amestolkiae: An enzyme displaying regioselective transxylosylation

49 p.-7 fig.-3 tab.

Detalles Bibliográficos
Autores: Nieto-Domínguez, Manuel José, Eugenio, Laura I. de, Barriuso, Jorge, Prieto Orzanco, Alicia, Fernández de Toro, Beatriz, Canales, Ángeles, Martínez, María Jesús
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/127076
Acceso en línea:http://hdl.handle.net/10261/127076
Access Level:acceso abierto
Palabra clave:Lignocellulosic biomass
Xylan
Enzyme
Ascomycetes
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spelling Novel pH-stable glycoside hydrolase family 3 β-xylosidase from Talaromyces amestolkiae: An enzyme displaying regioselective transxylosylationA new fungal B-xylosidase of biotechnological interestNieto-Domínguez, Manuel JoséEugenio, Laura I. deBarriuso, JorgePrieto Orzanco, AliciaFernández de Toro, BeatrizCanales, ÁngelesMartínez, María JesúsLignocellulosic biomassXylanEnzymeAscomycetes49 p.-7 fig.-3 tab.This paper reports on a novel β-xylosidase from the hemicellulolytic fungus Talaromyces amestolkiae. The expression of this enzyme, called BxTW1, could be induced by beechwood xylan and was purified as a glycoprotein from culture supernatants. We characterized the gene encoding this enzyme as an intronless gene belonging to the glycoside hydrolase gene family 3 (GH3). BxTW1 exhibited transxylosylation activity in a regioselective way. This feature would allow the synthesis of oligosaccharides or other compounds not available from natural sources, such as alkyl glycosides displaying antimicrobial or surfactant properties. Regioselective transxylosylation, an uncommon combination, makes the synthesis reproducible, which is desirable for its potential industrial application. BxTW1 showed high pH stability and Cu2+ tolerance. The enzyme displayed a pI of 7.6, a molecular mass around 200 kDa in its active dimeric form, and Km and Vmax values of 0.17 mM and 52.0 U/mg, respectively, using commercial p-nitrophenyl-β-D-xylopyranoside as the substrate. The catalytic efficiencies for the hydrolysis of xylooligosaccharides were remarkably high, making it suitable for different applications in food and bioenergy industries.This work was carried out with funding from the MINECO projects PRI-PIBAR-2011-1402 and RTC-2014-1777-3 and the Comunidad de Madrid Program RETO-PROSOT S2013/MAE-2907.Peer reviewedAmerican Society for MicrobiologyMinisterio de Economía y Competitividad (España)Comunidad de MadridConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/127076reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTC-2014-1777-3http://dx.doi.org/10.1128/AEM.01744-15Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1270762026-05-22T06:33:51Z
dc.title.none.fl_str_mv Novel pH-stable glycoside hydrolase family 3 β-xylosidase from Talaromyces amestolkiae: An enzyme displaying regioselective transxylosylation
A new fungal B-xylosidase of biotechnological interest
title Novel pH-stable glycoside hydrolase family 3 β-xylosidase from Talaromyces amestolkiae: An enzyme displaying regioselective transxylosylation
spellingShingle Novel pH-stable glycoside hydrolase family 3 β-xylosidase from Talaromyces amestolkiae: An enzyme displaying regioselective transxylosylation
Nieto-Domínguez, Manuel José
Lignocellulosic biomass
Xylan
Enzyme
Ascomycetes
title_short Novel pH-stable glycoside hydrolase family 3 β-xylosidase from Talaromyces amestolkiae: An enzyme displaying regioselective transxylosylation
title_full Novel pH-stable glycoside hydrolase family 3 β-xylosidase from Talaromyces amestolkiae: An enzyme displaying regioselective transxylosylation
title_fullStr Novel pH-stable glycoside hydrolase family 3 β-xylosidase from Talaromyces amestolkiae: An enzyme displaying regioselective transxylosylation
title_full_unstemmed Novel pH-stable glycoside hydrolase family 3 β-xylosidase from Talaromyces amestolkiae: An enzyme displaying regioselective transxylosylation
title_sort Novel pH-stable glycoside hydrolase family 3 β-xylosidase from Talaromyces amestolkiae: An enzyme displaying regioselective transxylosylation
dc.creator.none.fl_str_mv Nieto-Domínguez, Manuel José
Eugenio, Laura I. de
Barriuso, Jorge
Prieto Orzanco, Alicia
Fernández de Toro, Beatriz
Canales, Ángeles
Martínez, María Jesús
author Nieto-Domínguez, Manuel José
author_facet Nieto-Domínguez, Manuel José
Eugenio, Laura I. de
Barriuso, Jorge
Prieto Orzanco, Alicia
Fernández de Toro, Beatriz
Canales, Ángeles
Martínez, María Jesús
author_role author
author2 Eugenio, Laura I. de
Barriuso, Jorge
Prieto Orzanco, Alicia
Fernández de Toro, Beatriz
Canales, Ángeles
Martínez, María Jesús
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Comunidad de Madrid
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Lignocellulosic biomass
Xylan
Enzyme
Ascomycetes
topic Lignocellulosic biomass
Xylan
Enzyme
Ascomycetes
description 49 p.-7 fig.-3 tab.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/127076
url http://hdl.handle.net/10261/127076
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTC-2014-1777-3
http://dx.doi.org/10.1128/AEM.01744-15
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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