Toxicity of superparamagnetic iron oxide nanoparticles to the microalga Chlamydomonas reinhardtii

Superparamagnetic iron oxide nanoparticles (SPION) have been widely studied for different biomedical and environmental applications. In this study we evaluated the toxicity and potential alterations of relevant physiological parameters caused to the microalga Chlamydomonas reinhardtii (C. reinhardti...

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Detalles Bibliográficos
Autores: Hurtado Gallego, Jara, Pulido Reyes, Gerardo, González Pleiter, Miguel, Salas, Gorka, Leganés Nieto, Francisco, Rosal, Roberto, Fernández Piñas, Francisca
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/695379
Acceso en línea:http://hdl.handle.net/10486/695379
https://dx.doi.org/10.1016/j.chemosphere.2019.124562
Access Level:acceso abierto
Palabra clave:Internalization
Microalgae
Shading effect
SPION
Toxicity mechanisms
Biología y Biomedicina / Biología
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spelling Toxicity of superparamagnetic iron oxide nanoparticles to the microalga Chlamydomonas reinhardtiiHurtado Gallego, JaraPulido Reyes, GerardoGonzález Pleiter, MiguelSalas, GorkaLeganés Nieto, FranciscoRosal, RobertoFernández Piñas, FranciscaInternalizationMicroalgaeShading effectSPIONToxicity mechanismsBiología y Biomedicina / BiologíaSuperparamagnetic iron oxide nanoparticles (SPION) have been widely studied for different biomedical and environmental applications. In this study we evaluated the toxicity and potential alterations of relevant physiological parameters caused to the microalga Chlamydomonas reinhardtii (C. reinhardtii) upon exposure to SPION. The results showed dose-dependent toxicity. A mechanistic study combining flow cytometry and physiological endpoints showed a toxic response consisting of a decrease in metabolic activity, increased oxidative stress and alterations in the mitochondrial membrane potential. Additionally, and due to the light absorption of SPION suspensions, we observed a significant shading effect, causing a marked decrease in photosynthetic activity. In this work, we demonstrated for the first time, the internalization of SPION by endocytosis in C. reinhardtii. These results demonstrated that SPION pose a potential risk for the environment if not managed properlyThis research is supported by CTM2013-45775-C2-1/2-R, CTM2016-74927-C2-1,2-R and MAT2015-71806-R grants from MINECOElsevierDepartamento de BiologíaFacultad de Ciencias20192019-08-10research articlehttp://purl.org/coar/resource_type/c_2df8fbb1AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/695379https://dx.doi.org/10.1016/j.chemosphere.2019.124562reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6953792026-06-23T12:46:27Z
dc.title.none.fl_str_mv Toxicity of superparamagnetic iron oxide nanoparticles to the microalga Chlamydomonas reinhardtii
title Toxicity of superparamagnetic iron oxide nanoparticles to the microalga Chlamydomonas reinhardtii
spellingShingle Toxicity of superparamagnetic iron oxide nanoparticles to the microalga Chlamydomonas reinhardtii
Hurtado Gallego, Jara
Internalization
Microalgae
Shading effect
SPION
Toxicity mechanisms
Biología y Biomedicina / Biología
title_short Toxicity of superparamagnetic iron oxide nanoparticles to the microalga Chlamydomonas reinhardtii
title_full Toxicity of superparamagnetic iron oxide nanoparticles to the microalga Chlamydomonas reinhardtii
title_fullStr Toxicity of superparamagnetic iron oxide nanoparticles to the microalga Chlamydomonas reinhardtii
title_full_unstemmed Toxicity of superparamagnetic iron oxide nanoparticles to the microalga Chlamydomonas reinhardtii
title_sort Toxicity of superparamagnetic iron oxide nanoparticles to the microalga Chlamydomonas reinhardtii
dc.creator.none.fl_str_mv Hurtado Gallego, Jara
Pulido Reyes, Gerardo
González Pleiter, Miguel
Salas, Gorka
Leganés Nieto, Francisco
Rosal, Roberto
Fernández Piñas, Francisca
author Hurtado Gallego, Jara
author_facet Hurtado Gallego, Jara
Pulido Reyes, Gerardo
González Pleiter, Miguel
Salas, Gorka
Leganés Nieto, Francisco
Rosal, Roberto
Fernández Piñas, Francisca
author_role author
author2 Pulido Reyes, Gerardo
González Pleiter, Miguel
Salas, Gorka
Leganés Nieto, Francisco
Rosal, Roberto
Fernández Piñas, Francisca
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Biología
Facultad de Ciencias
dc.subject.none.fl_str_mv Internalization
Microalgae
Shading effect
SPION
Toxicity mechanisms
Biología y Biomedicina / Biología
topic Internalization
Microalgae
Shading effect
SPION
Toxicity mechanisms
Biología y Biomedicina / Biología
description Superparamagnetic iron oxide nanoparticles (SPION) have been widely studied for different biomedical and environmental applications. In this study we evaluated the toxicity and potential alterations of relevant physiological parameters caused to the microalga Chlamydomonas reinhardtii (C. reinhardtii) upon exposure to SPION. The results showed dose-dependent toxicity. A mechanistic study combining flow cytometry and physiological endpoints showed a toxic response consisting of a decrease in metabolic activity, increased oxidative stress and alterations in the mitochondrial membrane potential. Additionally, and due to the light absorption of SPION suspensions, we observed a significant shading effect, causing a marked decrease in photosynthetic activity. In this work, we demonstrated for the first time, the internalization of SPION by endocytosis in C. reinhardtii. These results demonstrated that SPION pose a potential risk for the environment if not managed properly
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-08-10
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/695379
https://dx.doi.org/10.1016/j.chemosphere.2019.124562
url http://hdl.handle.net/10486/695379
https://dx.doi.org/10.1016/j.chemosphere.2019.124562
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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